Analysis of tetracycline residues in royal jelly by liquid chromatography–tandem mass spectrometry

Xu, Jinzhong; Ding, Tao; Wu, Bin; Yang, Wenquan; Zhang, Xiaoyan; Liu, Yan; Shen, Chongyu; Jiang, Yuan

doi:10.1016/j.jchromb.2008.04.027

Cited by 44 publications

(24 citation statements)

References 15 publications

(24 reference statements)

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“…This phenomenon was also found in the literature . Compared with other studies (30–50 , 2–45% ), the matrix effect obtained by our work is relatively small. It proved the selectivity of MOF–MIP is satisfactory.…”

Section: Resultssupporting

confidence: 80%

Analysis of tetracyclines from milk powder by molecularly imprinted solid‐phase dispersion based on a metal–organic framework followed by ultra high performance liquid chromatography with tandem mass spectrometry

Wang

Zhang

et al. 2018

J of Separation Science

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A novel molecularly imprinted polymer that could selectively recognize tetracyclines in milk powder was synthesized using a metal-organic framework as a support material, tetracycline as template molecule, and 3-aminophenylboronic acid as a functional monomer and a cross-linking agent. The novel molecularly imprinted polymer was characterized by Fourier transform infrared spectrometry, transmission electron microscopy, X-ray diffractometry, thermogravimetric analysis, and N adsorption/desorption measurements. The adsorption isotherms, adsorption kinetics, adsorption thermodynamics, and selective adsorption experiments of the novel molecularly imprinted polymer to tetracycline were also studied. The novel molecularly imprinted polymer was used as dispersant of matrix solid-phase dispersion to extraction tetracyclines. After that, the tetracyclines extracted from milk powder were determined by ultra high performance liquid chromatography with tandem mass spectrometry. Under the optimal conditions, the detection limits of tetracyclines were 0.217-0.318 ng/g. The relative standard deviations of intra- and interday precision ranged from 3.8 to 6.9% and from 2.8 to 7.4%, respectively. In all three concentration levels (1.0, 10, 50 ng/g), the recoveries of tetracyclines ranged from 84.7 to 93.9%. The method was successfully applied to the determination of tetracyclines in milk powder.

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Section: Resultssupporting

confidence: 80%

Analysis of tetracyclines from milk powder by molecularly imprinted solid‐phase dispersion based on a metal–organic framework followed by ultra high performance liquid chromatography with tandem mass spectrometry

Wang

Zhang

et al. 2018

J of Separation Science

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“…At the end of the separation, the mobile phase returned to its initial composition (5% acetonitrile) for 3 min and the analytical column was equilibrated. In this way, a complete cycle of the online MISPE extraction and HPLC separation lasted for 18 min, which was shorter than that of traditional offline techniques (about 40 min) [7]. The peak areas were calculated at 355 nm and were used for data evaluation.…”

Section: Online Mispe-hplc-dad Proceduresmentioning

confidence: 99%

Online coupling of molecularly imprinted solid‐phase extraction to HPLC for determination of trace tetracycline antibiotic residues in egg samples

Niu

Xia

et al. 2011

J of Separation Science

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An automated system has been developed for the determination of trace tetracycline antibiotics (TCs) in egg samples, based on online molecularly imprinted solid-phase extraction (MISPE) coupling with high-performance liquid chromatography (HPLC). Oxytetracycline and chlortetracycline were chosen as mixed templates to synthesize highly selective molecularly imprinted polymers for online extraction. Under the optimal online MISPE-HPLC condition, 10 mL egg samples were injected into the MISPE column and then the matrix was washed out. By rotating the switching valve, TCs were transferred to the analytical column and then separated by HPLC. Because sample pretreatment and chromatographic separation were carried out simultaneously, the whole analytical time (18 min) was significantly shortened compared with conventional offline techniques. The detection limits ranged from 0.8 to 1.3 ng/g. The enhancement factors were in the range of 159-410. The spiked recoveries of TCs in real egg samples ranged from 91.6 to 107.6% and the relative standard deviations (RSDs) were not higher than 4.0%.

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“…Warm water was employed to disperse the royal jelly sample, because organic solvents such as methanol and acetonitrile are not miscible with royal jelly (Jin et al, ). Royal jelly is rich in proteins (Xu et al, ), which are different from those in other types of honey products. Various extraction solutions, including 1% TFA, 1% formic acid and the commonly used 0.2 m citric acid (Bohm, Stachel, & Gowik, ) were tested, and 1% TFA with 0.1 m Na 2 EDTA was found to be the best candidate for extraction of tested antibiotics from royal jelly.…”

Section: Resultsmentioning

confidence: 99%

Development and validation of a simple solid‐phase extraction method coupled with liquid chromatography–triple quadrupole tandem mass spectrometry for simultaneous determination of lincomycin, tylosin A and tylosin B in royal jelly

Zheng

Park

El‐Aty

et al. 2017

Biomedical Chromatography

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We have developed an analytical method for the determination of lincomycin, tylosin A and tylosin B residues in royal jelly using liquid chromatography-triple quadrupole tandem mass spectrometry analysis. For extraction and purification, we employed 1% trifluoroacetic acid and 0.1 m Na EDTA solutions along with an Oasis HLB cartridge. The target antibiotics were well separated in a Kinetex EVO C reversed-phase analytical column using a combination of 0.1% formate acid in ultrapure water (A) and acetonitrile (B) as the mobile phase. Good linearity was achieved over the tested concentration range (5-50 μg/kg) in matrix-matched standard calibration. The coefficients of determination (R ) were 0.9933, 0.9933 and 0.996, for tylosin A, tylosin B and lincomycin, respectively. Fortified royal jelly spiked with three different concentrations of the tested antibiotics (5, 10 and 20 μg/kg) yielded recoveries in the range 80.94-109.26% with relative standard deviations ≤4%. The proposed method was applied to monitor 11 brand of royal jelly collected from domestic markets and an imported brand from New Zealand; all the samples tested negative for lincomycin, tylosin A and tylosin B residues. In conclusion, 1% trifluoroacetic acid and 0.1 m Na EDTA aqueous solvents combined with solid-phase extraction could effectively complete the sample preparation process for royal jelly before analysis. The developed approach can be applied for a routine analysis of lincomycin, tylosin A and tylosin B residues in royal jelly.

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Analysis of tetracycline residues in royal jelly by liquid chromatography–tandem mass spectrometry

Cited by 44 publications

References 15 publications

Analysis of tetracyclines from milk powder by molecularly imprinted solid‐phase dispersion based on a metal–organic framework followed by ultra high performance liquid chromatography with tandem mass spectrometry

Analysis of tetracyclines from milk powder by molecularly imprinted solid‐phase dispersion based on a metal–organic framework followed by ultra high performance liquid chromatography with tandem mass spectrometry

Online coupling of molecularly imprinted solid‐phase extraction to HPLC for determination of trace tetracycline antibiotic residues in egg samples

Development and validation of a simple solid‐phase extraction method coupled with liquid chromatography–triple quadrupole tandem mass spectrometry for simultaneous determination of lincomycin, tylosin A and tylosin B in royal jelly

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