Analysis of Tetracycline Antibiotics Using HPLC with Pulsed Amperometric Detection

Charoenraks, Thiraporn; Chuanuwatanakul, Suchada; Honda, Kensuke; Yamaguchi, Yoko; Chailapakul, Orawon

doi:10.2116/analsci.21.241

Cited by 43 publications

(25 citation statements)

References 33 publications

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“…Previous works have shown that pulsed potential cleaning, as carried out in PAD technique, is an effective method for electropolishing in analysis of tetracycline [35], doxycycline and chlortetracycline [36]. Some authors have reported use of the PAD technique for BDD and GC electrodes [35,37].…”

Section: Electropolishing By Pad and Waveform Optimizationmentioning

confidence: 99%

Pulsed Amperometry for Anti-fouling of Boron-doped Diamond in Electroanalysis of β-Agonists: Application to Flow Injection for Pharmaceutical Analysis

Karuwan

Mantim

Chaisuwan

et al. 2006

Sensors

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This work presents the construction and application of boron-doped diamond (BDD) thin film electrode as sensor for the determination of three β-agonists, viz. salbutamol, terbutaline and clenbuterol. Although well-known as a chemically inert material, BDD film however shows fouling in detection of these compounds using fixedpotential mode amperometry. A suitable waveform for pulsed amperometric detection (PAD) was developed and used to determine the agonist compounds. It was seen that the developed PAD significantly refreshed the BDD surface for long-term detection in flow injection analysis. Linear working ranges were 0.5-100 µM, 1.0-100 µM and 0.5-50 µM for salbutamol, terbutaline and clenbuterol, respectively. The developed PAD-BDD system was applied to successfully determine salbutamol and terbutaline in commercial pharmaceutical products. The methods were validated with a capillary electrophoresis method.

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Section: Electropolishing By Pad and Waveform Optimizationmentioning

confidence: 99%

Pulsed Amperometry for Anti-fouling of Boron-doped Diamond in Electroanalysis of β-Agonists: Application to Flow Injection for Pharmaceutical Analysis

Karuwan

Mantim

Chaisuwan

et al. 2006

Sensors

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“…33,34,[45][46][47][48][49][50][51][52][53][54][55][56][57] Nestes sistemas, a principal função da detecção pulsada foi promover a limpeza eletroquímica constante e ativação da superfície do eletrodo de trabalho. A amplitude, polaridade e tempos de aplicação dos pulsos de potenciais, de detecção, limpeza e ativação podem ser variados, no sentido de se garantir a maior sensibilidade e estabilidade do sinal eletroquímico para uma diversidade de analitos.…”

Section: Limpeza E Ativação Da Superfície Do Eletrodo Através Da Mpaunclassified

Análise por injeção em fluxo com detecção amperométrica de múltiplos pulsos: potencialidades e aplicações

Santos¹,

Gimenes²,

Richter³

et al. 2011

Quím. Nova

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Recebido em 29/11/10; aceito em 15/3/11; publicado na web em 5/5/11 FLOW INJECTION ANALYSIS WITH MULTIPLE PULSE AMPEROMETRIC DETECTION: POTENTIALITIES AND APPLICATIONS. The potentialities and applications of the Multiple Pulse Amperometric detection (MPA) coupled with Flow Injection Analysis (FIA) are evaluated. Important aspects as cleaning and activation of electrode surface, indirect and simultaneous analysis of electroactive compounds and the use of the internal standard method for quantifications utilizing FIA-MPA are presented. The main parameters concerning the detection of electroactive analytes by multiple pulse amperometric detection in flowing solutions were also discussed. In addition, aspects such as flow rate, sample volume, application time of the potential pulses and instrumentation necessary for implementing of the method were also addressed.Keywords: electrochemical detection; multiple pulse amperometry; flow injection analysis. INTRODUÇÃOO desenvolvimento de métodos analíticos que combinam menor tempo de análise, maior sensibilidade e seletividade, simplicidade de manuseio, baixo custo e potencialidade adequada para automação constitui-se num campo de pesquisa amplo e em constante expansão na área de Química Analítica. Nesse contexto, a análise por injeção em fluxo, FIA -Flow Injection Analysis, tem sido explorada com sucesso na análise de grande diversidade de substâncias, proporcionando várias vantagens, tais como, uso de instrumentação versátil, baixo custo dos componentes do sistema, elevada frequência de amostragem, consumo reduzido de reagentes e amostras e, consequentemente, redução na geração de resíduos. 1-7 Além disto, o sistema FIA pode ser automatizado, minimizando os riscos de intoxicação do analista devido a uma menor exposição aos reagentes e amostras em comparação aos procedimentos manuais. Os primeiros trabalhos empregando FIA foram propostos por Rüzicka e Hansen, em 1975, como um novo conceito de análises químicas. 8,9 No primeiro artigo, os autores descrevem o sistema FIA acoplado a detectores de absorção molecular. 8 Três anos depois, os mesmos autores fazem uma revisão sobre os dez trabalhos que haviam desenvolvido anteriormente, abordando a teoria e as tendências da técnica. 9 Posteriormente, uma diversidade de técnicas foram exploradas, cabendo destacar a absorção atômica, 10,11 fluorescência, 12,13 quimiluminescência, 14,15 potenciometria, 16,17 voltametria, 18,19 amperometria, 20,21 entre outros.A detecção eletroquímica pode oferecer vantagens frente a outros sistemas de detecção, como baixo custo de instrumentação, simplicidade de aplicação e minimização de etapas de preparação de amostras. 3,[22][23][24][25] Adicionalmente, diversas espécies a serem analisadas podem ser pré-concentradas sobre eletrodos, tanto por processos de adsorção como por eletrodeposição. Os detectores eletroquímicos mais utilizados em sistemas em fluxo são os amperométricos, que foram adotados em grande parte dos trabalhos descritos na literatura. 3 Nesta técnica, o eletrodo de trabalho (ou se...

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“…Different analytical techniques have been developed for determination of terbutaline sulfate and tetracycline. HPLC, 8,9 LC-MS, 10 CE, 11 CE-MS, 12 voltammetry, 13 electrochemical method, 14,15 liquid chromatography, 16,17 capillary electrophoresis 18 and chemiluminescence 19,20 have been reported for determination of tetracycline. These methods are often time-consuming, expensive, and cumbersome.…”

Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric Determination of Tetracycline and Terbutaline Sulphate in Its Pure and Dosage Forms Using Eosin Y Reagent

Al-Sabha¹,

Dhamra²,

Al-Ghabsha³

2017

ECB

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A spectrofluorimetric method has been developed for the determination of tetracycline hydrochloride and terbutaline sulphate in different dosage forms. The method is based on the quantitative quenching effect of tetracycline hydrochloride and terbutaline sulphate on the native fluorescence of Eosin Y at the pH 6.4 and 3.5 respectively. The quenching of the fluorescence of Eosin Y was measured at 545 nm after excitation at 350 nm. The fluorescence-concentration plots are rectilinear over the range 0.5-18 and 0.05-5.0 μg mL -1 with LOD of 0.531 and 0.241 μg mL -1 and LOQ of 1.77 and 0.806 μg mL -1 for above drugs respectively. The proposed method has been successfully applied to the analysis of commercial tablets and capsules containing the drug. Statistical comparison of the results with those of the reference method revealed good agreement and proved that there were no significant differences in the accuracy and precision between the two methods.* Corresponding Authors E-Mail: dr_theiaa@yahoo.co.uk [a]

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Analysis of Tetracycline Antibiotics Using HPLC with Pulsed Amperometric Detection

Cited by 43 publications

References 33 publications

Pulsed Amperometry for Anti-fouling of Boron-doped Diamond in Electroanalysis of β-Agonists: Application to Flow Injection for Pharmaceutical Analysis

Pulsed Amperometry for Anti-fouling of Boron-doped Diamond in Electroanalysis of β-Agonists: Application to Flow Injection for Pharmaceutical Analysis

Análise por injeção em fluxo com detecção amperométrica de múltiplos pulsos: potencialidades e aplicações

Spectrofluorimetric Determination of Tetracycline and Terbutaline Sulphate in Its Pure and Dosage Forms Using Eosin Y Reagent

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