Analysis of Simvastatin using a Simple and Fast High Performance Liquid Chromatography-Ultra Violet Method: Development, Validation and Application in Solubility Studies

Munir, Abubakar; Ahmad, Mahmood; Malik, Muhammad Zubair; Minhas, Muhammad Usman

doi:10.4314/tjpr.v13i1.19

Cited by 9 publications

(8 citation statements)

References 14 publications

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“…2) Method development Different ratios 30:70 to 70:30 of Acetonitrile and pH6.5 phosphate buffer was used as mobile phase and 70:30 of Acetonitrile:pH 6.5 phosphate buffer selected as an appropriate mobile phase which gave a peak with better retention time and acceptable system suitability parameters was chosen for validation and solubility studies (Munir et al, 2014),(D. Gowri sankar et al, 2011) (Sanjay & Kumar, 2012), (Mohd. et al, 2011).…”

Section: B Methods 1) Chromatographic System and Conditionsmentioning

confidence: 99%

“…The working standard of 100µg/ml obtained by diluting the stock solution by acetonitrile. The working standard is diluted to obtain the concentrations ranging from 2-14µg/ml (2, 4, 6, 8, 10, 12 and 14µg/ml) were subjected to analysis by the proposed method (Gorain et al, 2013), (Munir et al, 2014), (Madishetty& Bontha, 2015). The optimized mobile phase ratio is used for calibration curve construction with concentration of Gefitinib on x-axis and peak area on y-axis.…”

Section: ) Methods Validation A) Selectivity and Specificitymentioning

confidence: 99%

“…LOD is the lowest concentration can be estimated but not necessarily quantified under the stated experimental conditions. LOQ is lowest concentration of an analyte that can estimated with acceptable precision and accuracy (Munir et al, 2014).…”

Section: E) Limit Of Detection and Limit Of Quantificationmentioning

confidence: 99%

“…To determine robustness of the present developed method, the flow rate was studied at 0.8ml/min and 1.2ml/min, effect of the change in wavelength was analysed at 247nm and 251nm and effect of mobile phase composition was assessed at 75:25 and 65:35 of Acetonitrile: pH 6.5 phosphate buffer. The percent RSD of robustness trial under these conditions are calculated (Munir et al, 2014).…”

Section: F) Robustnessmentioning

confidence: 99%

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Solubility Studies of Gefitinib by Validated High Pressure Liquid Chromatographic Method

Vijetha¹,

Reddy²

2020

JSR

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Section: B Methods 1) Chromatographic System and Conditionsmentioning

confidence: 99%

Section: ) Methods Validation A) Selectivity and Specificitymentioning

confidence: 99%

Section: E) Limit Of Detection and Limit Of Quantificationmentioning

confidence: 99%

Section: F) Robustnessmentioning

confidence: 99%

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Solubility Studies of Gefitinib by Validated High Pressure Liquid Chromatographic Method

Vijetha¹,

Reddy²

2020

JSR

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“…It belongs to the well‐known class of statins; hypolipidemics (cholesterol lowering) [8]. This semi‐synthetic prodrug is transformed to its β‐hydroxyacid form that inhibits 3‐hydroxy‐ 3‐methylglutaryl coenzyme‐A reductase (HMGCoA) and thus decreases low‐density lipoprotein, triglycerides, and increases high‐density lipoproteins [9,10].…”

Section: Introductionmentioning

confidence: 99%

Validated green micellar electrokinetic capillary chromatography for simultaneous determination of simvastatin and sitagliptin phosphate binary mixture in pharmaceutical formulation

Kamal

Morshedy

Saad

et al. 2021

Separation Science Plus

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Diabetics are liable to fatal atherosclerotic cardiovascular diseases. It is clinically attributed to their imbalanced plasma lipoproteins. Fixed‐dose combination “polypill” of simvastatin (10 mg) and sitagliptin phosphate (100 mg) is mainly indicated for the treatment of diabetic dyslipidemia. Novel green micellar electrokinetic capillary chromatographic method has been developed for simultaneous determination of simvastatin and sitagliptin phosphate in binary synthetic mixtures and their pharmaceutical formulation. Separation was achieved using a deactivated fused silica capillary (50 cm effective length × 50 μm id) through a background running electrolyte of 100 mM borate buffer (pH 9.1) and 100 mM sodium dodecyl sulfate. Diode array detection was set at 238 and 210 nm for simvastatin and sitagliptin phosphate, respectively. All experimental parameters were closely varied and optimized. Both drugs declared good linearities over the range of 10–100 μg/mL. Proposed study was validated according to International Council for Harmonization guidelines. It was applied to the assay of laboratory‐made binary mixtures and marketed tablets. Electrophoretic assay was compared with a reported spectrophotometric one. Satisfactory t‐test and F‐ratio indicated insignificant differences between both methods. Moreover, it is the first capillary electrophoretic simultaneous assay for simvastatin and sitagliptin phosphate, and fits a linear correlation with the green analytical attributes.

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Green analytical method development for statin analysis

Assassi¹,

Roy²,

Perovitch³

et al. 2015

Journal of Chromatography A

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Analysis of Simvastatin using a Simple and Fast High Performance Liquid Chromatography-Ultra Violet Method: Development, Validation and Application in Solubility Studies

Cited by 9 publications

References 14 publications

Solubility Studies of Gefitinib by Validated High Pressure Liquid Chromatographic Method

Solubility Studies of Gefitinib by Validated High Pressure Liquid Chromatographic Method

Validated green micellar electrokinetic capillary chromatography for simultaneous determination of simvastatin and sitagliptin phosphate binary mixture in pharmaceutical formulation

Green analytical method development for statin analysis

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