Analysis of quetiapine in human plasma using fluorescence spectroscopy

Mostafa, Islam M.; Omar, Mahmoud A.; Nagy, Dalia M.; Derayea, Sayed M.

doi:10.1016/j.saa.2018.02.019

Cited by 18 publications

(11 citation statements)

References 19 publications

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“…Direct protein precipitation with acetonitrile, methanol, and (35% w /w) perchloric acid gave low recoveries but was unable to eliminate protein interference from plasma. Although liquid–liquid extraction (LLE) and solid phase extraction (SPE) techniques had been used in previous studies reports, they were not given priority because they were time consuming and needed expensive materials. In the current study, SALLE was tested using different salts (sodium chloride, sodium hydroxide, sodium carbonate and potassium carbonate) at different amounts and different ratios.…”

Section: Resultsmentioning

confidence: 99%

“…Accordingly, it needs to be derivatized first with a fluorogenic reagent to enhance the sensitivity required for its bioanalysis. 4-Chloro-7-nitrobenzofurazan (NBD-Cl) is a well known, stable, nonfluorescent reagent that is utilized frequently as a derivatizing agent for quantification of several drugs containing the amino group [9][10][11][12] . In the current study, NBD-Cl has been selected as it is very reactive under mild reaction conditions, has a long shelf life, is available in any analytical laboratory, and is cheap and highly photostable compared with other existing derivatizing reagent such as fluorescamine and dansyl chloride.…”

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confidence: 99%

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Novel spectrofluorimetric quantification of alogliptin benzoate in biofluids exploiting its interaction with 4‐chloro‐7‐nitrobenzofurazan

Aref¹,

Hammad

Darwish

et al. 2019

Luminescence

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The direct determination of alogliptin benzoate (ALO) using fluorescence has not yet been accomplished because ALO cannot fluoresce naturally. Accordingly, it should be derivatized first with a fluorogenic reagent to enhance the sensitivity required for its bioanalysis. This method is the first spectrofluorimetric assay for ALO quantification exploiting the nucleophilic nature of its amino group to react with 4‐chloro‐7‐nitrobenzofurazan (NBD‐Cl) in borate buffer at pH 8.5 to produce a strong fluorescent compound that is excited at and emits at wavelengths 470 and 527 nm, respectively. Experimental variables concerning the conditions of reaction and fluorogenic intensity were carefully investigated and optimized. Linearity was from 1–250 ng ml−1 with a lower detection limit of 0.29 ng ml−1 and a lower quantification limit of 0.88 ng ml−1. Validation of the current study was accomplished with mean per cent recovery of 100.62 ± 1.59 in tablets and 99.86 ± 0.82 in human plasma. Furthermore, the current method has been utilized in the bioanalysis of ALO in real rat plasma after oral administration with a simple specimen preparation. The developed method has proven to be a promising alternative method for ALO analysis in bioequivalence studies.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Novel spectrofluorimetric quantification of alogliptin benzoate in biofluids exploiting its interaction with 4‐chloro‐7‐nitrobenzofurazan

Aref¹,

Hammad

Darwish

et al. 2019

Luminescence

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“…Many pharmaceutical compounds [17][18][19] were determined spectrophotometrically and/or spectrouorimetri-cally by utilizing chromogenic and uorogenic properties of NBD-Cl. The interaction between NBD-Cl with compounds bearing basic primary, secondary amino group or hydroxyl group resulted in the formation of colored and/or uorescent product.…”

Section: Resultsmentioning

confidence: 99%

Utility of the chromogenic and fluorogenic properties of benzofurazan for the assay of milnacipran in human urine and plasma

et al. 2018

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Our article presents the development and validation of two simple, very sensitive, and low-cost spectroscopic methods for the assay of milnacipran hydrochloride in bulk form, pharmaceutical tablets and spiked human urine and plasma. Spectroscopic methods (spectrophotometric and spectrofluorimetric techniques) were dependent on the chromogenic and fluorogenic properties of the 4-chloro-7 nitrobenzofurazan (NBD-Cl) reagent. The reaction product, resulting from the interaction between NBD-Cl and milnacipran in the presence of borate buffer pH 8.5, was measured spectrophotometrically at 465 nm and spectrofluorimetrically at 510 nm after excitation at 465 nm. The absorbance-concentration plot was rectilinear over the range of 1.5-12 mg mL À1 with a limit of quantitation 1.09 mg mL À1, while the fluorescence-concentration plot was rectilinear over the range of 0.03-0.5 mg mL À1 with a limit of quantitation 0.02 mg mL À1. Influential parameters affecting the development and stability of the reaction product were studied and optimized. Assurance of the cited drug in its tablets by our proposed methods was successfully completed without obstruction from the presence of the basic excipients with average percentage recoveries of 99.27 AE 1.18 and 99.44 AE 0.69for the spectrophotometric and spectrofluorimetric methods, respectively. The spectrofluorimetric method was additionally adopted as a preliminary in vitro study for the assay of the cited drug in spiked human urine and plasma with average percentage recoveries of 101.52 AE 1.01 and 100.38 AE 1.57 for spiked urine and plasma, respectively.

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“…NBD‐Cl has been used for the spectrofluorimetric determination of quetiapine fumarate which has a similar structure and pharmacological effect as our studied drug, also NBD‐Cl has been used for the assay of several pharmaceutical compounds with different structures and pharmacological effects . The free electron lone pair of nitrogen of the amino group present in these compounds attacked the chloride of NBD‐Cl.…”

Section: Resultsmentioning

confidence: 99%

Benzofurazan‐based fluorophore for the selective determination of flupentixol dihydrochloride: Application to content uniformity testing

et al. 2018

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One of the most commonly used drugs in treatment of schizophrenia is flupentixol dihydrochloride, therefore it is important to develop a simple, low cost and sensitive spectrofluorimetric method for the estimation of flupentixol dihydrochloride. The yellow fluorescent product that is generated from the nucleophilic substitution reaction of the free lone pair of the alcoholic hydroxyl group of the drug and 4-chloro-7-nitrobenzofurazan (NBD-Cl) in Mcllvaine buffer pH 7.0 was estimated at 510 nm (λ 460 nm). The variables that affect the development of the reaction product were explored and optimized. The linear range of this method was 0.5-2.5 μg ml with a limit of quantitation equal to 0.29 μg ml . Our method was successfully applied for the assurance of flupentixol in tablet form with average percentage recovery of 99.08 ± 1.01% without obstruction from the basic excipients exhibits. Furthermore, our strategy was extended to study the content uniformity testing of flupentixol in Fluaxnol tablets.

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Analysis of quetiapine in human plasma using fluorescence spectroscopy

Cited by 18 publications

References 19 publications

Novel spectrofluorimetric quantification of alogliptin benzoate in biofluids exploiting its interaction with 4‐chloro‐7‐nitrobenzofurazan

Novel spectrofluorimetric quantification of alogliptin benzoate in biofluids exploiting its interaction with 4‐chloro‐7‐nitrobenzofurazan

Utility of the chromogenic and fluorogenic properties of benzofurazan for the assay of milnacipran in human urine and plasma

Benzofurazan‐based fluorophore for the selective determination of flupentixol dihydrochloride: Application to content uniformity testing

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