Analysis of polymorphisms in the <i>insulin‐like growth factor 1 receptor</i> (<i>IGF1R</i>) gene from Japanese quail selected for body weight

Moe, Hla Hla; Shimogiri, Takeshi; Kamihiraguma, W.; Isobe, H.; Kawabe, Kentaro; Okamoto, Susumu; Minvielle, Francis; Maeda, Yoshizane

doi:10.1111/j.1365-2052.2007.01653.x

Cited by 17 publications

(13 citation statements)

References 9 publications

(8 reference statements)

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“…Since by using PCR-RFLP method the presence or absence of a restriction site is detected, two alleles are expected at a particular site [24][25][26] . However, if there are two …”

Section: Discussionmentioning

confidence: 99%

Investigation on the Polymorphism of Some Loci by Using PCR-RFLP in Japanese Quails (Coturnix coturnix japonica) Raised in Different Locations of Turkey

Bozkaya¹,

Gürler²,

Yertürk³

2013

Kafkas Univ Vet Fak Derg

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The aim of this study was to investigate the restriction fragment length polymorphism (RFLP) of some loci on chromosomes 1 (CJA1) and 3 (CJA3) in Japanese quails (Coturnix coturnix japonica) raised in Turkey. With this study population genetics parameters were estimated in order to select individuals for establishing a reference population, which would be used for studies on recombination frequency. Fertilized eggs obtained from flocks raised in six different provinces of Turkey were incubated for 120 h and 191 embryos were collected. From the tissue samples of the embryos, DNA was isolated by using DNA isolation kits. Particular regions of SEMA3E, IFR1, HAL, LOC396025, UGP2, LOC396192, TLX and BMP5 loci were amplified with specifically designed primers for each locus by using PCR technique. The PCR products were cut with an appropriate restriction enzyme for each locus and analysed by using agarose gel electrophoresis. Presence of different alleles, allele and genotype frequencies, heterozygosities and genetic distances were estimated. Out of the eight loci studied, polymorphism was found for the SEMA3E and TLX loci on 1. and 3. chromosomes, respectively, while five loci were found to be monomorphic and one locus (HAL) could not be amplified by PCR. The populations studied were found to be mostly in Hardy-Weinberg equilibrium. The results indicated that the SEMA3E and TLX loci can be used for studying recombination frequencies in the populations included into the study. Keywords: Japanese quail, RFLP, Polymorphism, RecombinationTürkiye'deki Japon Bıldırcınlarında (Coturnix coturnix japonica) Bazı Lokuslardaki Polimorfizmin PCR-RFLP Yöntemi İle Araştırılması ÖzetBu çalışmanın amacı Japon bıldırcınlarında (Coturnix coturnix japonica) 1. (CJA1) ve 3. (CJA3) kromozom üzerinde bulunan bazı genlerdeki kesim bölgesi polimorfizminin (RFLP) araştırılmasıdır. Bu çalışma ile rekombinasyon oranları ile ilgili araştırma amacıyla kullanılacak bir popülasyonu oluşturacak bireylerin seçimine temel teşkil edecek olan popülasyon genetiği parametrelerinin ortaya konması amaçlanmıştır. Türkiyenin altı farklı ilindeki işletmelerden elde edilen döllü bıldırcın yumurtaları 37°C'de 120 saat süreyle inkube edildikten sonra 191 adet embriyodan doku örneği alınmıştır. Alınan doku örneklerinden özel kitler yardımıyla DNA izolasyonu yapılmıştır. SEMA3E, IFR1, HAL, LOC396025, UGP2, LOC396192, TLX ve BMP5 lokuslarının belirli bölgeleri özel olarak dizayn edilmiş olan primerler yardımıyla ve PCR işlemi ile çoğaltılmıştır. Elde edilen PCR ürünleri uygun bir restririksiyon enzimi ile kesilmiş ve agaroz jel elektroforezi yardımıyla ayrımlanmıştır. İncelenen popülasyonlarda farklı allellerin varlığı, genotip ve allel frekansları, heterozigotluk dereceleri ve genetik mesafeler hesaplanmıştır. Çalışmada kullanılan sekiz lokustan 1. ve 3. kromozomlar üzerinde bulunan SEMA3E ve TLX lokuslarında polimorfizm tespit edilmiş, beş lokus monomorfik olarak bulunmuş bir lokusta (HAL) ise PCR işlemi ile çoğaltma yapılamamıştır. Çalışılan popülasyonların büyük...

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“…Since by using PCR-RFLP method the presence or absence of a restriction site is detected, two alleles are expected at a particular site [24][25][26] . However, if there are two …”

Section: Discussionmentioning

confidence: 99%

Investigation on the Polymorphism of Some Loci by Using PCR-RFLP in Japanese Quails (Coturnix coturnix japonica) Raised in Different Locations of Turkey

Bozkaya¹,

Gürler²,

Yertürk³

2013

Kafkas Univ Vet Fak Derg

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“…A total of 11 novel polymorphisms of the IGF-1R gene were detected in the Egyptian water buffalo by El-Magd et al (2013). Moe et al (2007) specified the existence of 21 SNPs by sequence comparison of the IGF1R coding region between LL and SS birds. Three SNPs (G26333A, G263336A, and C26639T) were detected in exon 2 of IGF-1R in Jinghai Yellow chickens by Yang et al (2012).…”

Section: Polymorphisms Of the Igf-1r Genementioning

confidence: 99%

Polymorphisms of AluI and Hin1I loci of the IGF-1R gene and their genetic effects on growth traits in Bian chickens

Peng¹,

Wang

Jin³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Growth traits are important economic traits in broiler chicken production. AluI and Hin1I loci are two restriction sites, which are respectively located in exons 2 and 3 of the IGF-1R gene. These two loci are significantly related to the growth traits in Jinghai Yellow chickens. In this study, a correlation analysis was performed between these two loci and the growth traits of Bian chickens. The results showed a G376A mutation at the AluI site and a C919A mutation at the Hin1I site, which respectively resulted in three genotypes AA, AB, and BB in exon 2 and three genotypes CC, CD, and DD in exon 3. Correlation analysis showed that the female Bian chickens with the AA genotype of the AluI locus had higher body weights than those with the 2 P.F. Wu et al. Genetics and Molecular Research 16 (2): gmr16029619AB genotype (P < 0.05) at 8, 14, 16, and 18 weeks; individuals with CD genotype of Hin1I locus had higher body weights at 6, 8, 10, 12, and 14 weeks compared to the CC genotype (P < 0.05 or P < 0.01). Combined genotypes analysis showed that at the age of 8, 14, 16, and 18 weeks, the body weight of AACC genotype combination was higher than that of the ABCC genotype combination (P < 0.05); at the age of 6, 8, 12, 14, 16, and 18 weeks, the AACD genotype combination had higher (P < 0.05 or P < 0.01) body weight than that of the ABCC genotype.

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“…To detect loci that affect growth traits in the Japanese quail, we used an F 2 population from an intercross between the LL and SS lines that was constructed previously by Moe et al (2007). The F 2 generation, which had 75 males and 74 females, was generated by mating a single F 1 male with two F 1 females.…”

Section: Detection Of Loci That Affect Growth Traits In the Japanese mentioning

confidence: 99%

“…Recently, we reported that a nonsynonymous single nucleotide polymorphism (SNP) in the gene for insulin-like growth factor 1 receptor (IGF1R) was associated with body weight in the LL and SS lines (Moe et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Detection of a Polymorphism Associated with Shank Length and Body Weight in Japanese Quail (Coturnix japonica) by AFLP

et al. 2012

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In this study, we attempted to use amplified fragment length polymorphism (AFLP) in combination with selective genotyping to detect loci that affected shank length and body weight at 10 weeks of age in Japanese quail (Coturnix japonica). A total of 88 primer pairs were used in AFLP analyses of 10 and 13 males with the longest and shortest shank lengths, respectively, from an F 2 intercross population derived from lines differentiated by large and small body size (LL and SS, respectively). Ten AFLP bands differed strikingly in frequency between the two groups (P＜0.005). Genotyping of the 10 bands in 10 females with the longest shank lengths and 10 with the shortest revealed that one band differed significantly in frequency between the two groups for both males and females (P＜ 0.05). This fragment shared 86.4% identity with a 361-bp sequence of chicken chromosome 1 located from position 51,672,791 to 51,673,152, as determined by chicken BLAT search. Polymerase chain reaction and sequence analysis revealed that a 190-bp insertion/deletion was the causal polymorphism. Genotypes of this indel marker were associated significantly with shank length and body weight in the F 2 intercross population (P＜0.005). The insertion allele had a positive effect on both traits. To confirm the association of this marker with the two traits, we conducted association analysis in a population derived from heterozygous quails of another line. In this analysis, the indel genotypes showed no significant association with shank length or male body weight (P＞0.05), but did show a significant association with female body weight (P＜0.05).

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Analysis of polymorphisms in the insulin‐like growth factor 1 receptor (IGF1R) gene from Japanese quail selected for body weight

Cited by 17 publications

References 9 publications

Investigation on the Polymorphism of Some Loci by Using PCR-RFLP in Japanese Quails (Coturnix coturnix japonica) Raised in Different Locations of Turkey

Investigation on the Polymorphism of Some Loci by Using PCR-RFLP in Japanese Quails (Coturnix coturnix japonica) Raised in Different Locations of Turkey

Polymorphisms of AluI and Hin1I loci of the IGF-1R gene and their genetic effects on growth traits in Bian chickens

Detection of a Polymorphism Associated with Shank Length and Body Weight in Japanese Quail (Coturnix japonica) by AFLP

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