Analysis of Phosphatidylcholines Alterations in Human Glioblastomas Ex Vivo

Pekov, Stanislav I.; Sorokin, Anatoly; Kuzin, Andrey A.; Bocharov, K. V.; Bormotov, Denis S.; Shivalin, A S; Shurkhay, Vsevolod; Потапов, А. А.; Nikolaev, Eugene; Popov, Igor

doi:10.1134/s1990750821030070

Cited by 9 publications

(7 citation statements)

References 24 publications

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“…In this study, we use the Inline Cartridge Extraction (ICE) [14] ambient ionization mass spectrometry technique to rapidly obtain molecular profiles of brain tissues. Similar to many other ambient ionization methods [5,6], it precludes lipid identification (see Figures S1 and S2 in Supplementary Materials) and relies on the analysis of the sample's total molecular profile, including the lipid component [4], which is the predominant component of molecular profiles obtained by ambient ionization mass spectrometry [10,11]. Multiple techniques for intraoperative tumor tissue classification [9,10] are based on a multivariate statistical analysis of the tissue profile, rather than on the identification of the molecular nature of the detected ions.…”

Section: Resultsmentioning

confidence: 99%

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Determination of Brain Tissue Samples Storage Conditions for Reproducible Intraoperative Lipid Profiling

et al. 2022

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Ex-vivo molecular profiling has recently emerged as a promising method for intraoperative tissue identification, especially in neurosurgery. The short-term storage of resected samples at room temperature is proposed to have negligible influence on the lipid molecular profiles. However, a detailed investigation of short-term molecular profile stability is required to implement molecular profiling in a clinic. This study evaluates the effect of storage media, temperature, and washing solution to determine conditions that provide stable and reproducible molecular profiles, with the help of ambient ionization mass spectrometry using rat cerebral cortex as model brain tissue samples. Utilizing normal saline for sample storage and washing media shows a positive effect on the reproducibility of the spectra; however, the refrigeration shows a negligible effect on the spectral similarity. Thus, it was demonstrated that up to hour-long storage in normal saline, even at room temperature, ensures the acquisition of representative molecular profiles using ambient ionization mass spectrometry.

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Section: Resultsmentioning

confidence: 99%

“…The metabolism alterations that accompany malignancy allows to implement rapid molecular techniques in clinical practice [1][2][3][4]. A variety of ambient mass spectrometry methods are proposed to discriminate cancer tissues intraoperatively [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Determination of Brain Tissue Samples Storage Conditions for Reproducible Intraoperative Lipid Profiling

et al. 2022

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“…Numerous lipid species became dysregulated in various cancer types [2]. However, at this moment, only some generic trends in upregulation of mono and diunsaturated phosphatidylcholines are observed across various diagnoses, in particular, in glioblastoma multiform [2,3], which attracts interest in the investigation of lipid composition alterations occurring during malignancy.…”

Section: Discussionmentioning

confidence: 99%

“…In brain tumors, as in many other proliferating cells, anaerobic glycolysis becomes the major pathway of glucose metabolism, which is called the Warburg effect [4]. The high rate of proliferation specific to malignant tissues requires a considerable amount of biomass components to support the growth and formation of new cells, and therefore the promotion of de novo lipogenesis, especially the synthesis of phospholipids to build cell membranes and triglycerides required for energy storage and production [3,5,6]. The lipid composition of cancer cells is different from healthy ones due to many factors.…”

Section: Discussionmentioning

confidence: 99%

Lipid Profiles of Human Brain Tumors Obtained by High-Resolution Negative Mode Ambient Mass Spectrometry

Zavorotnyuk

Pekov

Sorokin

et al. 2021

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Alterations in cell metabolism, including changes in lipid composition occurring during malignancy, are well characterized for various tumor types. However, a significant part of studies that deal with brain tumors have been performed using cell cultures and animal models. Here, we present a dataset of 124 high-resolution negative ionization mode lipid profiles of human brain tumors resected during neurosurgery. The dataset is supplemented with 38 non-tumor pathological brain tissue samples resected during elective surgery. The change in lipid composition alterations of brain tumors enables the possibility of discriminating between malignant and healthy tissues with the implementation of ambient mass spectrometry. On the other hand, the collection of clinical samples allows the comparison of the metabolism alteration patterns in animal models or in vitro models with natural tumor samples ex vivo. The presented dataset is intended to be a data sample for bioinformaticians to test various data analysis techniques with ambient mass spectrometry profiles, or to be a source of clinically relevant data for lipidomic research in oncology.

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“…Клиническая липидомика является разделом метаболомики, основной аналитический инструмент которого -хромато-масс-спектрометрия. Из-за широкого аналитического охвата липидов вместе с высокой чувствительностью и молекулярной специфичностью становится возможным зафиксировать дисбаланс липидов в измененных клеточных мембранах и липидных каплях, в том числе и на этапе ранней диагностики клинически невыраженных состояний [4,5].…”

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Lipidomic markers of obesity and their dynamics after bariatric surgery

Saprina,

Bashirova,

Ivanov

et al. 2024

Bûll. sib. med.

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Obesity is considered as a chronic progressive disease, heterogeneous in its etiology and clinical manifestations, and characterized by excess in body fat mass and its deposition in the body. The term “morbid obesity” refers to excessive deposition of adipose tissue with a body mass index (BMI) ≥40 kg / m2 or with a BMI ≥ 35 kg / m2 in the presence of serious complications associated with obesity. Along with obesity, the frequency of type 2 diabetes mellitus and cardiovascular diseases closely associated with it has increased. It results from the progression of metabolic disorders, including insulin resistance, which is inextricably linked with the accumulation of visceral fat and plays a key role in the pathogenesis of obesity-related diseases.The study of lipidomic signatures in obesity and associated conditions is a promising branch of fundamental medicine, which makes it possible to significantly and at a new conceptual level stratify a cohort of obese patients into various phenotypes, including a metabolically healthy and metabolically unhealthy obesity phenotypes. Dynamic changes in the lipidome both in the context of diet, drug treatment, and after various bariatric surgeries are of great interest for developing personalized strategies for the treatment of this disease. Currently available studies and their results suggest that we are only at the very start of studying this promising biomedical field.

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Analysis of Phosphatidylcholines Alterations in Human Glioblastomas Ex Vivo

Cited by 9 publications

References 24 publications

Determination of Brain Tissue Samples Storage Conditions for Reproducible Intraoperative Lipid Profiling

Determination of Brain Tissue Samples Storage Conditions for Reproducible Intraoperative Lipid Profiling

Lipid Profiles of Human Brain Tumors Obtained by High-Resolution Negative Mode Ambient Mass Spectrometry

Lipidomic markers of obesity and their dynamics after bariatric surgery

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