Lipid Profiles of Human Brain Tumors Obtained by High-Resolution Negative Mode Ambient Mass Spectrometry

Abstract

Alterations in cell metabolism, including changes in lipid composition occurring during malignancy, are well characterized for various tumor types. However, a significant part of studies that deal with brain tumors have been performed using cell cultures and animal models. Here, we present a dataset of 124 high-resolution negative ionization mode lipid profiles of human brain tumors resected during neurosurgery. The dataset is supplemented with 38 non-tumor pathological brain tissue samples resected during elective surgery. The change in lipid composition alterations of brain tumors enables the possibility of discriminating between malignant and healthy tissues with the implementation of ambient mass spectrometry. On the other hand, the collection of clinical samples allows the comparison of the metabolism alteration patterns in animal models or in vitro models with natural tumor samples ex vivo. The presented dataset is intended to be a data sample for bioinformaticians to test various data analysis techniques with ambient mass spectrometry profiles, or to be a source of clinically relevant data for lipidomic research in oncology.

Determination of Brain Tissue Samples Storage Conditions for Reproducible Intraoperative Lipid Profiling

“…The intraoperative methods used for cancer tissue discrimination rely not on the specific biomarkers, but also on the complex analysis of the molecular profiles [25] of the analyzed tissue. In this study, we use the Inline Cartridge Extraction (ICE) [14] ambient ionization mass spectrometry technique to rapidly obtain molecular profiles of brain tissues.…”

Incorporation of a Disposable ESI Emitter into Inline Cartridge Extraction Mass Spectrometry Improves Throughput and Spectra Stability

“…Currently, intraoperative assessment of tumor extent is performed using frozen section histopathology, a time- and labor-intensive technique. Thus, there is a need for rapid tissue diagnosis for identifying tumor margins and controlling the volume of excised tissue. , Several research groups have successfully utilized various direct mass spectrometry (MS) methods to provide extensive molecular information on the sampled tissue in a few minutes or faster. − In most cases, MS analysis is focused on the lipid profile, which undergoes significant changes during malignancy. − Some of these methods are capable of providing information in real time but require installing a mass spectrometer in every operating room. Other methods ,− , can be directly integrated into histopathological routines for suitability screening, significantly expediting them while requiring only one mass spectrometer per hospital.…”