Analysis of Mixtures of Sugars and Mixtures of Amino Acids by Dialysis Based on Differential Kinetics.

Spectrophotometric analyses of ribose and 2-deoxy-d-ribose alone and in mixtures

“…Differences in color development after reaction with specific reagents have been another approach (6). Dialysis based on differential kinetics (7) and combinations of different methods (8) have been proposed. However, some of these techniques are nonselective and are insensitive to small sugar concentrations.…”

Dialysis culture of microorganisms: design, theory, and results.

“…If a mixture of compounds is dialyzed, the individual components will diffuse through the membrane at different rates, even with the same concentration driving force across the membrane. Craig (27) developed this principle for the separation of small protein fragments, and Siggia et al (149) used it to measure the relative amounts of sugars or amino acids in a mixture.…”

Nature of hafnium-chloranilic acid metallochrome by matrix rank, contour mapping, and iterative analysis of absorption spectra

“…Chloranilic acid (l,4-dichloro-2,5-dihydroxyquinone) as a colorimetric reagent for hafnium was of interest because it formed the colored complexes at high acidities where the hydrolysis of hafnium did not interfere. Previous studies on hafnium (3,4) had shown that hydrolysis was negligible in 3M perchloric acid and this medium at 25°C was used here.…”

“…The first series of 12 solutions containing only chloranilic acid in perchloric acid was prepared using a 25-ml precision buret to introduce 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, and 24 ml of the 1.00 X 10™ 4 567M chloranilic acid into 25-ml volumetric flasks and then filling to the mark with 3M perchloric acid. Thus solutions were prepared containing 0.08 to 0.96 X 10-4M chloranilic acid in steps of 0.08 X 10-4M.…”