Analysis of membrane interactions of antibiotic peptides using ITC and biosensor measurements

Abstract

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Association of vancomycin with lipid vesicles

“…Fitting of the data into one-site model gave rise to millimolar level affinity (dissociate constant, K d ), suggesting that vancomycin weakly, but definitely interact with liposome. The observation is in line with previous studies, where weak binding of vancomycin to model membrane were assessed by ITC [18] and surface plasmon resonance [19]. …”

“…We have shown that glycopeptide antibiotics vancomycin associate with biomimetic membrane, a fact have been largely ignored in the field because of its weak interaction [18,19]. Furthermore, we showed for the first time that it binds to membrane vesicle derived from gram-positive bacteria Staphylococcus aureus in a similar fashion.…”

Biosensors in Antimicrobial Drug Discovery: Since Biology until Screening Platforms

“…Between them Surface Plasmon Resonance (SPR) biosensor is an optical system where plasmons are excited and transmitted across a coating with ligand that interacts with an analyte in a fluid [ [54]. For the study of interactions between biomolecules and microbial cell membrane, that can predict antimicrobial activity and action mechanisms, SAW biosensors show the ability of measure mass and viscoelastic properties of microbial monolayer on sensor surface, by determining of impedance changes determined by microbial metabolism [55], in that way interactions on bacterial membranes of gallidermin and vancomycin was studied with SAW biosensors for identify the binding of antimicrobial peptides in bacterial cells [56,57]. Also, SAW biosensors have achieved to measure the growth in 7 hours of E. coli, providing the ability to perform monitoring of microbial growth in real time and can be adapted to a remote query wireless for use in dangerous environments [58].…”

Biosensor Applications in the Field of Antibiotic Research—A Review of Recent Developments

“…However, the mass sensitive sensors alone were not sufficient enough to fully elucidate the target independent interaction with model membranes. Therefore, SAW was combined with isothermal titration calorimetry (ITC) to finally reveal that the insertion of gallidermin into the membrane and the resultant influence on the dynamic membrane properties is a serious contribution to the gallidermin activity [58]. The above mentioned studies illustrate that biosensors are useful tools to elucidate the antibiotic mode of action of different compounds.…”