Association of vancomycin with lipid vesicles

Hu, Xiaohui; Tam, Kin Yip

doi:10.5599/admet.5.3.400

Cited by 3 publications

(5 citation statements)

References 26 publications

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“…This is possibly due to combined electrostatic and intermolecular hydrogen bond interaction between the hydroxyl head group of PG and vancomycin which is the main driving force for vancomycin association. [57] Although, we did not observe a large increase in resistance upon vancomycin titration, the relative change in capacitance (ΔQ) on the other hand increases (Figure 2b), suggesting some membrane thinning. This result is further supported by a decrease in membrane homogeneity (Figure 4) values (Dm).…”

Section: Discussionmentioning

confidence: 70%

“…This is consistent with the reported mode of action of the drug, and is also consistent with a related report on vancomycin interaction with liposome carried out using fluorescence. [57] Note that the binding curve did not follow a typical Langmuir-Freundlich isotherm curve as reflected from a larger index of heterogeneity, n value (n~2.5). This behavior might be due to unfavorable adsorption of vancomycin at the exofacial (distal leaflet).…”

Section: Discussionmentioning

confidence: 97%

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The Impact of Membrane Composition and Co‐Drug Synergistic Effects on Vancomycin Association with Model Membranes from Electrochemical Impedance Spectroscopy

2020

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The interaction of the antibacterial drug, vancomycin with microcavity suspended lipid bilayers (MSLB) was investigated using non‐Faradaic electrochemical impedance spectroscopy (EIS). Five MSLB compositions were prepared with increasing complexity at gold substrates: DOPC, DOPC:Chol, DOPC:SM:Chol, mammalian plasma membrane mimetic (MPM), and E. coli polar lipid extract. The latter two, are intended to mimic eukaryotic and bacterial inner membrane compositions respectively. The extent of vancomycin association and the impact drug association has on the electrochemical properties of the membrane depended on biomembrane composition. Trends were similar across all membranes but the E. coli membrane composition. Vancomycin increased membrane resistance and decreased capacitance in a saturable, concentration dependent manner, but for E. coli membrane resistance change was negligible and capacitance increased. Membrane resistance data was fit to the Langmuir‐Freundlich model to give quantitative insight into the relative extent of association of drug with membrane as a function of membrane composition. Overall, electrochemical data indicates that vancomycin associates at the interface of lipid membranes rather than penetrates the layer and this is promoted by the presence of anionic phospholipid. Interestingly, co‐incubation of the E. coli extract bilayer with both rifampicin and vancomycin, known to act synergistically, significantly promotes vancomycin association to E. coli membrane.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 97%

The Impact of Membrane Composition and Co‐Drug Synergistic Effects on Vancomycin Association with Model Membranes from Electrochemical Impedance Spectroscopy

2020

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“…We observed that vancomycin strongly co-localised with the mitochondria and nucleus, as well as the plasma membrane ( Fig 5A ). Vancomycin has previously been shown to have an affinity for the lipid phosphatidylglycerol [PG] (Hu and Tam, 2017), a lipid species found within the inner mitochondrial membrane (Schenkel and Bakovic, 2014). PG is the precursor to the mitochondrial-specific lipid cardiolipin, which plays crucial roles in stabilising the electron transport chain and cristae formation within mitochondria (Falabella et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

“…Using fluorescently labelled vancomycin as a probe, we found that vancomycin bound to multiple structures within macrophages including mitochondria and the plasma membrane. Previous work that examined vancomycin binding to liposomes showed that the lipid PG had the greatest affinity for vancomycin binding, but that binding capacity was highly dependent on the head groups of the lipids and the curvature of membrane (Hu and Tam, 2017). PG is transported to the inner mitochondrial membrane and acts as a precursor for cardiolipin biosynthesis, a lipid found exclusively within mitochondria.…”

Section: Discussionmentioning

confidence: 99%

Vancomycin impairs macrophage fungal killing by disrupting mitochondrial morphology and function

Bojang,

Sheriff,

et al. 2024

Preprint

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Vancomycin is a widely prescribed antibiotic used in the treatment of Gram-positive bacterial infections. We recently showed that this antibiotic disrupted protective anti-fungal immune responses via microbiome dysbiosis, enhancing susceptibility to invasive candidiasis. Antibiotics are an independent risk factor for developing this life-threatening fungal infection, but whether microbiota-independent mechanisms also drive this association is not clear. Here, we show that vancomycin directly impairs macrophage responses toCandida albicans, the main causative agent of invasive candidiasis. Vancomycin-treated macrophages were less able to killC. albicansdespite normal phagocytosis rates and were hyper-inflammatory and more likely to die during infection. We found that vancomycin bound to macrophage mitochondria, leading to depolarisation, reduced respiratory capacity and a hyper-fragmented morphology associated with increased ROS production. Taken together, this work demonstrates direct effects of vancomycin on mammalian immune cells, helping us to understand pro-inflammatory effects of this drug and how it promotes susceptibility to life-threatening fungal infection.

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“…VANKA associates with liposome through its interaction with the head group of the lipids. The previous studies state that it is unlikely for the VANKA to penetrate deep into the lipids bilayer this means that VANKA does not interact and bind to lipid tails [37,38].…”

Section: Particle Size Distribution and Morphologymentioning

confidence: 99%

Development of Vancomycin Delivery Systems Based on Autologous 3D Platelet-Rich Fibrin Matrices for Bone Tissue Engineering

et al. 2021

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Autologous platelet-rich fibrin (PRF) is derived from the blood and its use in the bone tissue engineering has emerged as an effective strategy for novel drug and growth factor delivery systems. Studies have approved that combined therapy with PRF ensures higher biological outcomes, but patients still undergo additional treatment with antibiotic drugs before, during, and even after the implantation of biomaterials with PRF. These systematically used drugs spread throughout the blood and lead not only to positive effects but may also induce adverse side effects on healthy tissues. Vancomycin hydrochloride (VANKA) is used to treat severe Staphylococcal infections but its absorption in the target tissue after oral administration is low; therefore, in this study, we have developed and analyzed two kinds of VANKA carriers—liposomes and microparticles in 3D PRF matrices. The adjustment, characterization, and analysis of VANKA carriers in 3D PRF scaffolds is carried out in terms of encapsulation efficiency, drug release kinetics and antibacterial activity; furthermore, we have studied the micro- and macrostructure of the scaffolds with microtomography.

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Association of vancomycin with lipid vesicles

Abstract: Antibiotics play a pivotal role in modern medicine for the treatment of bacterial infection in patients.

Cited by 3 publications

References 26 publications

The Impact of Membrane Composition and Co‐Drug Synergistic Effects on Vancomycin Association with Model Membranes from Electrochemical Impedance Spectroscopy

The Impact of Membrane Composition and Co‐Drug Synergistic Effects on Vancomycin Association with Model Membranes from Electrochemical Impedance Spectroscopy

Vancomycin impairs macrophage fungal killing by disrupting mitochondrial morphology and function

Development of Vancomycin Delivery Systems Based on Autologous 3D Platelet-Rich Fibrin Matrices for Bone Tissue Engineering

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