Analysis of Major Isoforms of Human Growth Hormone before and after Intensive Physical Exercise

Abstract

We developed a semiquantitative method for the analysis of main growth hormone isoforms. The use of immunoaffinity sorbents, two-dimensional electrophoresis, and immunoblotting allows detection of more than 90% circulating growth hormone. It was demonstrated that the proportion of growth hormone isoforms in human serum before and after strenuous exercise remained unchanged.

Relationship between Lactate Concentrations in Active Muscle Sweat and Whole Blood

“…The test with step-wise increasing power [10,11] served as the model exercise. Experiments were approved by the Ethic Committee of the Institute Physical Culture.…”

Noninvasive biochemical monitoring of physiological stress by Fourier transform infrared saliva spectroscopy

“…The GH concentration in blood serum was determined according to the method described in Ref. 38. All assays were performed three times.…”

“…The significant increase in the blood GH concentration after the ramp test indicates that the athletes have experienced a strong physiological stress. 38…”

Stability of Human Growth Hormone: Influence of Methionine Oxidation on Thermal Folding

“…hGH secretion in the body is pulsatile, it has a short life and presents peaks at intervals of about 3 to 5 h 1,6. Peak levels can vary between individuals and can be affected by several factors such as physical activity 7. The mean hGH secretion in adult males is in the range between 0.07 and 0.82 ng/mL,8 but this value can vary based on gender, obesity and age 9.…”