Analysis of madurose and other actinomycete whole cell sugars by gas chromatography

Saddler, G. S.; Tavecchia, Paolo; Lociuro, Sergio; Zanol, Margherita; Colombo, Luigi; Selva, Enrico

doi:10.1016/0167-7012(91)90022-i

Cited by 120 publications

(50 citation statements)

References 13 publications

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“…The molar ratios of amino acids were determined by gas chromatography-mass spectrometry of N-heptafluorobutyryl amino acid isobutyl esters as described previously (14). The whole-cell sugars were determined by gas chromatography of alditol acetates as described by Saddler et al (33). The glycolate content of bacterial cells was determined by the colorimetric method of Uchida and Aida (41).…”

Section: Methodsmentioning

confidence: 99%

Janibacter limosus gen. nov., sp. nov., a New Actinomycete with meso-Diaminopimelic Acid in the Cell Wall

Martin

Schümann

Rainey

et al. 1997

International Journal of Systematic Bacteriology

118

117

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New gram-positive bacteria were isolated from 1-year-old sludge from a wastewater treatment plant. The isolates are coccoid to rod-shaped, nonmotile aerobes that form neither spores nor mycelia. They are characterized by a peptidoglycan with directly cross-linked rneso-diaminopimelic acid (type Aly), by the presence of menaquinone MK-S(H,), and by the lack of mycolic acids. The strains have complex fatty acid patterns with i-C16:o and straight-chain saturated and unsaturated fatty acids as major components. The G+C content of the DNA is 70 mol%. The results of chemotaxonomic studies and a 16s ribosomal DNA sequence comparison support our proposal to assign these bacteria to a new genus, the genus Janibacter gen. nov.; the type species is Junibucter Zirnosus sp. nov., and the type strain of J. Zirnosus is strain HKI 83 (= DSM 11140).Both spore-forming and asporogenous actinomycetes have been screened during the last few years for useful bioactive compounds (13,39). The asporogenous organisms play important roles in mineralization of environmentally hazardous chemicals, such as polycyclic aromatic hydrocarbons (22), and in biotechnological production of natural products (23,26,29).To isolate new actinomycetes with new biological activities, we investigated several samples of soil, sludge, and sewage waste. A total of 168 strains were isolated from a 1-year-old sludge sample collected from a wastewater treatment plant. Two of these isolates were found to be markedly different from the other isolates and from previously described genera in their chemotaxonomic and their physiological features. In this paper these two strains are characterized phenotypically and phylogenetically. Below we propose the creation of a new genus, the genus Janibacter gen. nov., with one species, Janibacter limosus sp. nov., for these organisms. MATERIALS AND METHODSBacterial strains and cultural conditions. Strains HKI 83T (T = type strain) and HKI 84 were isolated from a 1-year-old sludge sample from the wastewater treatment plant near Jena, Thuringia, Germany, by the dilution plate technique on plate count agar (Difco Laboratories, Detroit, Mich.). General laboratory cultivation was performed at 28°C on solid rich medium (R medium) or in liquid R medium (43), which contained 1% (wtivol) Bacto Peptone (Difco), 0.5% (wt/vol) yeast extract, 0.5% (wt/vol) Casamino Acids, 0.2% (wthol) meat extract, 0.5% (wt/vol) malt extract, 0.2% (wtivol) glycerol. 0.1% (wt/vol) MgSO, -7H,O, and 0.005% (wt/vol) Tween 80 (pH 7.2). To determine the cellular fatty acids, strains were cultivated for 24 h in liquid tryptic soy broth (Difco) at 28°C.Morphological and physiological characteristics. Colony morphology on R medium and cell morphology at different ages were determined by stereomicroscopy and phase-contrast microscopy (Olympus, Tokyo, Japan). Nitrate reductase activity, urease activity, indole production, methyl red and Voges-Proskauer reactions, hydrogen sulfide production, and hydrolysis of esculin and Tween 80 were determined as described by Lanyi (...

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Section: Methodsmentioning

confidence: 99%

Janibacter limosus gen. nov., sp. nov., a New Actinomycete with meso-Diaminopimelic Acid in the Cell Wall

Martin

Schümann

Rainey

et al. 1997

International Journal of Systematic Bacteriology

118

117

View full text Add to dashboard Cite

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“…The fatty acid methyl esters were examined using the MIDI system (Sasser, 1990), and the composition of the mycolic acid methyl esters was determined using electronimpact mass spectrometry according to Collins et al (1982). The analysis of sugars as their alditol acetates was carried out after Saddler et al (1991).…”

Section: Methodsmentioning

confidence: 99%

Nocardia flavorosea sp. nov.

Chun

Seong

Bae

et al. 1998

International Journal of Systematic Bacteriology

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An actinomycete strain, 'Nocardia flavorosea' JCM 3332, was found to have properties consistent with its classification in the genus Nocardia. An almost complete gene sequence of the 165 rDNA of the strain was determined following cloning and sequencing of the amplified gene. The sequence was aligned with those available for nocardiae and phylogenetic trees were inferred using four tree-making algorithms. The organisms consistently formed a distinct clade with the type strain of Nocardia carnea. However, DNA relatedness experiments showed that the strain and N. carnea DSM 43397T belonged to two distinct genomic species. The organism was also distinguished from representatives of all of the validly described species of Nocardia using a combination of phenotypic properties. These genotypic and phenotypic data show that the strain merits recognition as a new species of the genus Nocardia. The name proposed for the new species is Nocardia flavorosea sp. nov. The type strain is JCM 333ZT.

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“…The procedures of Becker et al (1964) and Lechevalier & Lechevalier (1980) were used for the analysis of whole-cell composition. GC\MS was used for the quantitative determination of sugar content (Saddler et al, 1991). The phospholipid analysis was carried out by the method of Lechevalier et al (1981).…”

Section: Methodsmentioning

confidence: 99%

Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae.

Cui¹,

Mao²,

Zeng³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

309

149

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Analysis of madurose and other actinomycete whole cell sugars by gas chromatography

Cited by 120 publications

References 13 publications

Janibacter limosus gen. nov., sp. nov., a New Actinomycete with meso-Diaminopimelic Acid in the Cell Wall

Janibacter limosus gen. nov., sp. nov., a New Actinomycete with meso-Diaminopimelic Acid in the Cell Wall

Nocardia flavorosea sp. nov.

Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae.

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