Nocardia flavorosea sp. nov.

Abstract

An actinomycete strain, 'Nocardia flavorosea' JCM 3332, was found to have properties consistent with its classification in the genus Nocardia. An almost complete gene sequence of the 165 rDNA of the strain was determined following cloning and sequencing of the amplified gene. The sequence was aligned with those available for nocardiae and phylogenetic trees were inferred using four tree-making algorithms. The organisms consistently formed a distinct clade with the type strain of Nocardia carnea. However, DNA relatedness experiments showed that the strain and N. carnea DSM 43397T belonged to two distinct genomic species. The organism was also distinguished from representatives of all of the validly described species of Nocardia using a combination of phenotypic properties. These genotypic and phenotypic data show that the strain merits recognition as a new species of the genus Nocardia. The name proposed for the new species is Nocardia flavorosea sp. nov. The type strain is JCM 333ZT.

Leuconostoc kimchii sp. nov., a new species from kimchi.

“…DNA-DNA relatedness studies. DNA relatedness among strains was determined in duplicate experiments by using the membrane filter technique modified after Chun et al (1998). Genomic DNA (200 ng) was denatured by using the alkaline method and immobilized on nylon membrane (Hybond-N + ; Amersham) by applying a low vacuum.…”

Novel Bioactive Metabolites from a Marine Derived Bacterium Nocardia sp. ALAA 2000

“…16S rRNA gene amplification and sequencing from the test strain were carried out using a procedure of Chun et al [29], and Kim et al [30].…”

Nocardia puris sp. nov.

“…More critical examples are Nocardia veterana and N. africana, the 16S rDNA of which are 99?3 % similar, whereas the total labelled genomic DNA of the two species exhibits only 28?8 % homology. Similar situations were found for Nocardia brevicatena and Nocardia paucivorans, which have a 16S rDNA similarity value of 99?6 % but 61?9 % DNA-DNA relatedness (Yassin et al, 2000a), and for Nocardia carnea and Nocardia flavorosea, which have a 16S rRNA similarity value of 99?2 % but 5?0 % DNA-DNA relatedness (Chun et al, 1998). The 97 % limit is thus not always fulfilled in the genus Nocardia and additional distinctive characteristics must be determined to allow the definition of a new species.…”