Analysis of heterocyclic amines in hair by on-line in-tube solid-phase microextraction coupled with liquid chromatography−tandem mass spectrometry

High-throughput and sensitive analysis of urinary heterocyclic aromatic amines using isotope-dilution liquid chromatography–tandem mass spectrometry and robotic sample preparation system

“…Measurement of HCAA in hair could be an alternative analysis to determine the long-term exposure to HCAA. [29, 30]…”

Bioanalytical HPLC Applications of In-Tube Solid Phase Microextraction: A Two-Decade Overview

“…This problem was overcome with the use of Carboxen 1006 PLOT column, which also showed great extraction efficiency [31]. Supel Q PLOT columns have been successfully employed for the in-tube SPME of various organic compounds, including heterocyclic amines from hair [36], endocrine disruptors from liquid medicines and intravenous injection solutions [51], melatonin from saliva [52], cortisol from human saliva [53], urinary heterocyclic amines [54], testosterone, cortisol, and dehydroepiandrosterone from saliva [55], and anabolic steroids from urine [56].…”

“…Compared to the conventional WCOT liquid-phase GC columns, PLOT columns exhibit a larger adsorption surface and thicker film layer, resulting in higher extraction efficiency of the target analyte. Various PLOT columns have been evaluated, including Carboxen 1006 PLOT capillary column (carbon molecular sieves) (Supelco) [31,32,[48][49][50], Rt-U PLOT (divinylbenzene ethylene glycol/dimethylacrylate) (Restek (Bellefonte, PA, USA) [38], CP-Pora PLOT amine (basic modified stylene divinylbenzene polymer) (Varian Inc., Lake Forest, CA, USA) [48], and Supel Q PLOT (Divinylbenzene polymer) (Supelco) [32,36,48,[51][52][53][54][55][56].…”

Online capillary solid-phase microextraction coupled liquid chromatography-mass spectrometry for analysis of chiral secondary alcohol products in yeast catalyzed stereoselective reduction cell culture

“…This column has been used by researchers for extraction compounds such as steroid hormones [27,34,39,47], ochratoxins [32] and aflatoxins [45], emerging contaminants [33,40] and phthalate esters [40][41][42], and heterocyclic amines [46] that indicates an suitable choice for our application. Since the extraction solvent for SPME was deionized distilled water, a suitable desorption and elution solvent must bear some polar property to avoid producing inhomogeneous phase of the eluent with adsorbed residue water during analytes desorption and analysis.…”

“…Nowadays, the SPME technique has been extensively applied to various samples such as environmental waters [25][26][27][28][29][30][31], wine samples [22,23], nuts and grains [32], tea products and crude drugs [33], human urine [34][35][36][37][38], human saliva [38,39], liquid medicines and intravenous injection solutions [40], and human hair [35]. Recently, in order to increase the detection sensitivity for ultra-trace sample analysis, the SPME coupled liquid chromatography has switched its detector from spectrometers such as ultraviolet (UV) detector [30,37,[40][41][42] and fluorescence (FL) detector [33] to high sensitivity mass spectrometer (MS) [26,29,32,[34][35][36]38,39,[43][44][45] and tandem mass spectrometer (MS/MS) [25,27,28,31,46,47].…”