Analysis of hepatocyte growth factor immunostaining in the placenta of HIV-infected normotensive versus preeclamptic pregnant women

Cele, Sandile; Odun-Ayo, Frederick; Onyangunga, Onankoy; Moodley, J.; Naicker, Thajasvarie

doi:10.1016/j.ejogrb.2018.05.042

Cited by 5 publications

(3 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The human placenta is an hemochorial organ and is highly vascularized; yet, there are conflicting reports on the presence of lymphatic vessels in the placenta. However, Gu et al (2006) [65], Wang et al (2011) [66], and Liu et al (2015) [67], as well as our recent observations [68], do not confirm the presence of lymphatic vessels in the placenta. The aforementioned groups instead observed a stromal network immunostained with podoplanin.…”

Section: Lymphangiogenesiscontrasting

confidence: 71%

See 1 more Smart Citation

Angiogenesis, Lymphangiogenesis, and the Immune Response in South African Preeclamptic Women Receiving HAART

Naicker

Phoswa

Onyangunga

et al. 2019

IJMS

View full text Add to dashboard Cite

Purpose of the review: This review highlights the role of angiogenesis, lymphangiogenesis, and immune markers in human immunodeficiency virus (HIV)-associated preeclamptic (PE) pregnancies in an attempt to unravel the mysteries underlying the duality of both conditions in South Africa. Recent findings: Studies demonstrate that HIV-infected pregnant women develop PE at a lower frequency than uninfected women. In contrast, women receiving highly active anti-retroviral therapy (HAART) are more inclined to develop PE, stemming from an imbalance of angiogenesis, lymphangiogenesis, and immune response. Summary: In view of the paradoxical effect of HIV infection on PE development, this study examines angiogenesis, lymphangiogenesis, and immune markers in the highly HIV endemic area of KwaZulu-Natal. We believe that HAART re-constitutes the immune response in PE, thereby predisposing women to PE development. This susceptibility is due to an imbalance in the angiogenic/lymphangiogenic/immune response as compared to normotensive pregnant women. Further large-scale studies are urgently required to investigate the effect of the duration of HAART on PE development.

show abstract

Section: Lymphangiogenesiscontrasting

confidence: 71%

“…The aforementioned groups instead observed a stromal network immunostained with podoplanin. Lymphangiogenesis was observed at the decidua [68,69,70,71,72] and the uterine wall [64,73].…”

Section: Lymphangiogenesismentioning

confidence: 99%

Angiogenesis, Lymphangiogenesis, and the Immune Response in South African Preeclamptic Women Receiving HAART

Naicker

Phoswa

Onyangunga

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…Hepatocyte growth factor (HGF) is an essential regulator of placental development. Once Met binds to its ligand HGF, it is dimerized and phosphorylated, leading to its activation and stimulation of multiple downstream signaling pathways, including the MEK/Erk, phosphatidylinositol 3-kinase (PI-3K) and signal transduction and activators of transcription 3 (STAT3) pathways, which play important roles in the progression of trophoblast functions [ 6 , 7 ]. Deficiency of HGF or its receptor Met in mice causes fetal lethality and placental dysfunction [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Hypoxic stress disrupts HGF/Met signaling in human trophoblasts: implications for the pathogenesis of preeclampsia

Wang

Cao

et al. 2022

J Biomed Sci

View full text Add to dashboard Cite

Background Preeclampsia (PE), a placenta-associated pregnancy complication, is the leading cause of maternal and perinatal morbidity and mortality. Met/Erk signaling is inhibited in the placentas of patients with early-onset preeclampsia (E-PE), but the underlying mechanisms remain elusive. In this study, the expression modes of Met and endocytic vesicles in normal and preeclamptic placentas were compared. Biotinylation internalization/recycling assays were used to measure the endocytosis of Met under hypoxia and normoxia in HTR8/SVneo cells. In addition, the expression level of Cbl, a specific E3 ligase of Met, was measured under hypoxia and normoxia, and the endocytosis of Met was studied by using confocal microscopy. Results We found considerable intracellular accumulation of Met, which was colocalized with caveolin-1 (CAV-1), in trophoblasts from E-PE placentas. Prolonged hypoxic stimulation led to the remarkable augmentation of CAV-1-mediated Met endocytosis in HTR8/SVneo cells. In addition, the expression of Cbl was substantially repressed by sustained hypoxia, disrupting ubiquitin degradation and the subsequent intracellular accumulation of Met in HTR8/SVneo cells. The abnormal degradation of Met hampered the ability of hepatocyte growth factor (HGF) to promote trophoblast cell invasion. In E-PE placentas, aberrant upregulation of CAV-1 and downregulation of Cbl were observed in parallel to the intracellular accumulation of Met. Conclusions These findings reveal that prolonged hypoxic stress induces the augmentation of endocytosis and repression of ubiquitin-mediated Met degradation, which leads to the impaired regulation of trophoblast invasion by HGF/Met signaling. These data provide novel evidence for elucidating the pathogenesis of preeclampsia, especially of the early-onset subtype.

show abstract

Placental morphology, apoptosis, angiogenesis and epithelial mechanisms in early-onset preeclampsia

Travaglino

Raffone

Saccone

et al. 2019

European Journal of Obstetrics & Gynecology and Reproductiv

View full text Add to dashboard Cite

Analysis of hepatocyte growth factor immunostaining in the placenta of HIV-infected normotensive versus preeclamptic pregnant women

Cited by 5 publications

References 32 publications

Angiogenesis, Lymphangiogenesis, and the Immune Response in South African Preeclamptic Women Receiving HAART

Angiogenesis, Lymphangiogenesis, and the Immune Response in South African Preeclamptic Women Receiving HAART

Hypoxic stress disrupts HGF/Met signaling in human trophoblasts: implications for the pathogenesis of preeclampsia

Placental morphology, apoptosis, angiogenesis and epithelial mechanisms in early-onset preeclampsia

Contact Info

Product

Resources

About