Analysis of Flavonoids fromEugenia unifloraLeaves and Its Protective Effect against Murine Sepsis

Abstract

Eugenia uniflora, referred to as Pitanga cherry shrub, is largely distributed in tropical and subtropical America. This plant is cultivated in many countries and it is suitable for the production of juice, frozen pulp, and tea. Besides, it can be used as treatment for inflammatory diseases. We reported that a flavonoid-rich fraction (HE-Bu) obtained from leaves decreased the lethality induced by cecal ligation and puncture (CLP), a clinically relevant model of sepsis. The oral administration of HE-Bu reduced the late mortality rate by 30%, prevented neutrophil accumulation in lungs, decreased TNF-α and IL-1β serum levels, and markedly decreased iNOS and COX-2 protein expression by ileum cells. Chemical investigation showed myricetin and quercetin rhamnosides as the major components of this fraction. The results showed that HE-Bu protected mice from sepsis and indicated that this edible plant produces compounds that could be considered as potential adjuvants for sepsis treatment.

Antifungal activity of medicinal plants from Northeastern Brazil

“…Regarding the extracts from E. uniflora, the differences among antifungal assay procedures as well as the extraction parameters such as the solvent (aqueous or hydroalcoholic mixtures) and/or extraction procedures (infusion, decoction or maceration) may explain the better antifungal performance found in this study, in comparison with previous studies (Schapoval et al, 1994;Holetz et al, 2002). As well know, the extractive conditions (procedure e solvents) plays an important role on the extract chemical spectrums, in special the polyphenolic compounds from leaves of E. uniflora such as hydrolysable tannins (Lee et al, 1997(Lee et al, , 2000 and/or quercetin/ myricetin-glicosyl derivates (Rattman et al, 2012).…”

Antifungal and cytotoxicity activities of Banisteriopsis argyrophylla leaves

“…When these signals were subjected to sequential MS/MS (Figures –), they gave rise to the signal at m/z 300, attributed to homolytic cleavage between the aglycone moiety (in quercetins) and the respective glycosides, resulting in a radical aglycone that can be stabilized by resonance structures (Figure ). The signal at m/z 301 was correlated with neutral eliminations of glycosides and subsequently the signal at m/z 151 was attributed to the C ring opening through the retro RDA mechanism …”

“…The signal at m/z 301 was correlated with neutral eliminations of glycosides and subsequently the signal at m/z 151 was attributed to the C ring opening through the retro RDA mechanism. [33][34][35][36] The analysis of fraction A3 by (-)-HRESIMS ( Figure S17) showed the presence of four signals at m/z 431.0959 (compound 7), 433.0759 (compound 4 or 5), 447.0916 (6) and 599.1011 (compound 8 or 9) that were correlated with the flavonoids in the deprotonated form [M -H] À (Table 1). [37][38][39] The fragmentation pattern is similar to those discussed previously for these compounds.…”

Chemical Profiling of the Essential Oils of Syzygium aqueum, Syzygium samarangense and Eugenia uniflora and Their Discrimination Using Chemometric Analysis

“…Pitanga or Surinam cherry Eugenia uniflora L. is an evergreen tree, which is native to South America, but has been widely cultivated in the tropics . The essential oil from the leaves, which contains curzerene, γ ‐elemene, atractylone, trans ‐ β ‐elemenone, zerumbone, furanodiene, furanoelemene, trans ‐ β ‐ocimene, cis ‐ocimene, the isomeric β ‐ocimene, and β ‐pinene as major components, showed antioxidant, antimicrobial, anticancer, antinociceptive, and antiparasitic activities …”