Antifungal and cytotoxicity activities of Banisteriopsis argyrophylla leaves

Abstract

Objectives This work aimed to evaluate the antifungal and cytotoxic activity of the EtOH extract and fractions of Banisteriopsis argyrophylla leaves, and to perform the identification of these bioactive metabolites. Methods The EtOAc fraction (EAF) obtained from the ethanolic extract of B. argyrophylla leaves showed better antifungal potential against Candida spp. In this fraction, ten flavonoids have been identified by UHPLC‐ESI‐MSn. Then, EAF was submitted to column chromatography to give four new fractions (A1–A4). The cytotoxicity was determined against Vero cells. Key findings The EAF showed better antifungal potential against Candida spp. with minimum inhibitory concentrations (MICs) between 31.25 and 93.75 μg/ml. The (–)‐catechin (fraction A1) showed a MIC of 2.83 μg/ml against Candida glabrata. Fractions A2, A3 and A4 were rich in quercetins and kaempferols and showed good inhibitory concentrations (5.86–46.87 μg/ml) against C. albicans, C. glabrata and C. tropicalis. Conclusions The EtOH extract, fractions and the isolated (–)‐catechin showed lower toxicity to Vero cells than cisplatin, used as a positive control. Thus, the leaves of B. argyrophylla are a promising source of antifungal agents.

Dereplication by HPLC-ESI-MS and antioxidant activity of phenolic compounds from Banisteriopsis laevifolia (Malpighiaceae)

“…However, precise information on retention time would be necessary, as these dihydroxy acids have at least six isomers that present the m/z [M-H]and [M-CO 2 ] -(Belaya 2020). The family 2e presents a node identified as compound (9), which was obtained from EEL and EEF, with a quasi-molecular ion [M-H]m/z 289.073 and ions 245.082, 205.050, 203.071, 179.034, 125.023, and 109.028. Compounds (5) and (9) fragmentation profiles were also identified and reported for B. argyrophylla (Oliveira et al 2018). In addition, compounds (6) and (9) were also reported in B. laevifolia species (Nunes et al 2016).…”

Estudo químico e biológico das flores de Cassia bakeriana Craib

“…A formação deste íon ocorre através da eliminação neutra de 18 u (correspondente a H2O). A formação do íon em m/z 177 foi atribuído à clivagem do anel C através de um mecanismo retro-Diels-Alder (RDA) (DEMARQUE et al, 2016;OLIVEIRA et al, 2018).…”

“…Quanto a formação dos íons m/z 125, m/z 259 e m/z 241, a princípio ocorre clivagem heterocíclica no anel C para a formação do íon fragmento m/z 125, seguida de eliminação neutra de 178 u. Já a formação do íon 259, levou a eliminação neutra de 44 u (que corresponde ao CO2), através da descarboxilação. Os íons fragmentos m/z 241 e m/z 199 foram obtidos através da eliminação neutra de 18 u, que corresponde novamente a H2O, formando desta forma novo heterocíclo, altamente tensionado (DEMARQUE et al, 2016;OLIVEIRA et al, 2018;NASCIMENTO et al, 2020). A formação do íon fragmento m/z 217 ocorre através da contração do anel B, através da eliminação neutra de 58 u, formando assim novamente um novo heterocíclo altamente tensionado.…”