Analysis of electrode shape effect on single HeLa cell impedance using COMSOL simulation

Li, Jai-Hao; Chang, Wen-Hao; Wang, Min-Haw

doi:10.1109/bibm.2013.6732758

Cited by 3 publications

(11 citation statements)

References 4 publications

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“…Given the dimensions and applied voltages and ion concentration ranges considered, these effects should be minimal compared to the factors considered. Other groups have done similar simulations on IDE biosensors for detecting cells and bacteria [ 1 , 20 ], and for simulations involving other electrode configurations [ 21 , 22 ]. The uniqueness in the simulations presented here comes from the specific application using nanoparticle to reduce the measured impedance across electrodes and in using these simulations as a design tool in optimizing sensor function.…”

Section: Discussionmentioning

confidence: 99%

Simulations of Interdigitated Electrode Interactions with Gold Nanoparticles for Impedance-Based Biosensing Applications

MacKay

Hermansen

Wishart

et al. 2015

Sensors

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In this paper, we describe a point-of-care biosensor design. The uniqueness of our design is in its capability for detecting a wide variety of target biomolecules and the simplicity of nanoparticle enhanced electrical detection. The electrical properties of interdigitated electrodes (IDEs) and the mechanism for gold nanoparticle-enhanced impedance-based biosensor systems based on these electrodes are simulated using COMSOL Multiphysics software. Understanding these properties and how they can be affected is vital in designing effective biosensor devices. Simulations were used to show electrical screening develop over time for IDEs in a salt solution, as well as the electric field between individual digits of electrodes. Using these simulations, it was observed that gold nanoparticles bound closely to IDEs can lower the electric field magnitude between the digits of the electrode. The simulations are also shown to be a useful design tool in optimizing sensor function. Various different conditions, such as electrode dimensions and background ion concentrations, are shown to have a significant impact on the simulations.

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Section: Discussionmentioning

confidence: 99%

Simulations of Interdigitated Electrode Interactions with Gold Nanoparticles for Impedance-Based Biosensing Applications

MacKay

Hermansen

Wishart

et al. 2015

Sensors

View full text Add to dashboard Cite

show abstract

“…Other groups have done similar simulations on interdigitated electrode biosensors for detecting cells and bacteria [1], [15], and for simulations involving other electrode configurations [16], [17]. The uniqueness in the simulations presented here comes from the specific application using nanoparticle to reduce the measured impedance across electrodes and in using these simulations as a design tool in optimizing sensor function.…”

Section: Discussionmentioning

confidence: 99%

Simulating electrical properties of interdigitated electrode designs for impedance-based biosensing applications

MacKay

Hermansen

Wishart

et al. 2015

2015 IEEE 28th Canadian Conference on Electrical and Computer Engineering (CCECE)

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In this paper, we describe a point-of-care biosensor design. The uniqueness of our design is in its capability for detecting a wide variety of target biomolecules and the simplicity of nanoparticle enhanced electrical detection. The electrical properties of interdigitated electrodes and the mechanism for gold nanoparticle-enhanced impedance-based biosensor systems based on these electrodes were simulated using COMSOL Multiphysics software. Understanding these properties and how they can be affected by the design of the sensor, environment during detection, and target biomolecules is vital in designing effective biosensor devices. Simulations were used to show electrical screening for interdigitated electrodes in a salt solution as well as the electric field between individual digits of electrodes. Using these simulations, it was observed that gold nanoparticles bound closely to interdigitated electrodes can lower the electric field magnitude between the digits of the electrode.

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“…The electric field distribution determined by the electrode shape has large effects on the single cell impedance. 50 Thus, the uniformity of electric field distribution is quite significant in fabricating the microdevices for individual cell measurement. Assuming that the single cell is located in the center of the electrodes, the maxima of electric field strength are near the region of the cell−electrode boundary no matter how the electrodes are shaped.…”

Section: ■ Influence Factors Of Theoretical Modelmentioning

confidence: 99%

“…Uniformity of electric field distribution is very important for single cell measurement in microfluidic chips. 50 However, electrodes less than 50 μm will cause nonuniform distribution of the electric field. 49 We Figure 3.…”

mentioning

confidence: 99%

“…22 suggest that electrodes should be designed to be round, for the distribution of electric field provided by circular electrodes (see Figure 2a,b) is more uniform than that provided by electrodes with other shapes (see Figure 2c−e). 50 Interelectrode Distance. A large distance between working and counter electrodes will not influence cellular impedance measurement; 48 however, excessive interelectrode distance will degrade the detection efficiency of the ECIS device (see Figure 2a), for only cells adhered on the electrode can be detected, while insufficient interelectrode distance will cause the problem of electric current directly bypassing the cells adhered on electrodes.…”

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confidence: 99%

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How to Choose a Proper Theoretical Analysis Model Based on Cell Adhesion and Nonadhesion Impedance Measurement

Wei

Zhang

et al. 2021

ACS Sens.

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The accurate equivalent circuit model contributes to the better fitting of required cell characteristics, such as cell impedance, cell adhesion area, and cell–electrode distance. However, so many theoretical models on specific modules make it difficult for new researchers to understand the whole model of electrode system physically. Besides, the accurate theoretical model and the simplified calculations obviously contradict each other; therefore, it is confusing for many researchers to choose the proper theoretical model to calculate the specific parameters required. In this review, we first discuss the problems and suggestions of electrode system design for cell adhesion-based measurement in terms of parasitic capacitance, detection range of cell number, electric field distribution, and interelectrode distance. The design of electrode system for cell nonadhesion measurement was analyzed in terms of microchannel size and electrode position. Then, we discuss the advantages and disadvantages of various equivalent circuit models according to different requirements of researchers, and simultaneously provide a corresponding theoretical model for researchers. Various factors influencing electric impedance spectroscopy (EIS) such as the parasitic capacitance between microelectrodes, the changes of cell adhesion area and cell–electrode distance, the electrode geometry, and the surface conductivity of electrode were quantitatively analyzed to contribute to better understanding of the equivalent models. Finally, we gave advice to optimize the theoretical models further and perspectives on building uniform principles of theoretical model optimization in the future.

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Analysis of electrode shape effect on single HeLa cell impedance using COMSOL simulation

Cited by 3 publications

References 4 publications

Simulations of Interdigitated Electrode Interactions with Gold Nanoparticles for Impedance-Based Biosensing Applications

Simulations of Interdigitated Electrode Interactions with Gold Nanoparticles for Impedance-Based Biosensing Applications

Simulating electrical properties of interdigitated electrode designs for impedance-based biosensing applications

How to Choose a Proper Theoretical Analysis Model Based on Cell Adhesion and Nonadhesion Impedance Measurement

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