Analysis of cytoplasmic microtubules and flagellar roots in the zoospores ofAllomyces macrogynus

Aliaga, George R.; Pommerville, Jeffrey C.

doi:10.1007/bf01322631

“…The molecular machinery needed for zoospore motility and for zoospore germination, including undulopodia withdrawal, cyst-wall formation, and germ-tube initiation, is already packaged within the spore (Silva et al 1987). The flagellar apparatus is in the posterior end of the zoospore and typically consists of the kinetosome, a nonflagellated centriole (=secondary centriole) lying at an angle and anterior to the kinetosome, a multilayered striated rhizoplast (root) positioned perpendicular to the plane of flagellar beat and connected to the basal mitochondrion (Aliaga and Pommerville 1990), and microtubules typically arranged in nine groups of threes extending symmetrically from dense material around the kinetosome as a cage around the nucleus and nuclear cap. All ribosomes are aggregated into a nuclear cap and surrounded by a cisterna that is continuous with the outer membrane of the nuclear envelope (Fig.…”

Section: Zoospore Ultrastructurementioning

confidence: 99%

Blastocladiomycota

Powell¹

2016

Handbook of the Protists

2

0

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The Blastocladiomycota are posteriorly uniflagellated zoosporic fungi found as saprotrophs and parasites primarily in freshwater and soil. Once considered Chytridiomycota, phylogenetically they are a monophyletic group divergent from other zoosporic fungi, clustering among the nonzoosporic fungi. Their thalli range from monocentric, polycentric, tubular, to hyphal and are unusual among fungi in exhibiting alternation of a haploid gametophytic generation with a diploid sporophytic generation. Thick-walled resistant sporangia are the sites of meiosis and aid in the survival of the organism when environmental conditions become adverse. The hallmark of the group is the ultrastructural architecture of

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“…Microtubules have been observed in many fungi [1] such as in the aquatic moulds of the genus Allomyces in which they were found to be present in the subapical zone of the vegetative hyphae [2] and in the zoospores [3]. They are known to be involved in flagellar motility [4][5][6], chromosome segregation [7], maintenance of cellular morphology [8] and intracellular transport of organelles including mitochondria and vesicles Correspondence to: I.…”

Section: Introductionmentioning

confidence: 99%

Evolution of tubulin isoforms during the sporophytic development of Allomyces arbuscula

SCHONENBERGER

¹

1993

FEMS Microbiology Letters

0

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Tubulins extracted from the sporophytic developmental stages of Allomyces arbuscula have been characterised by one‐ and two‐dimensional SDS‐PAGE gels immunoblotted with monoclonal antibodies as α‐, acetylated α‐ (Mr 57 kDa both) and β‐ (Mr 55 kDa) isoforms. The zoosporangial isoforms could be characterised only when precautions were taken to inhibit the strong tubulin proteolytic activity at this stage. The zoospores and zoosporangia contained greater amounts of the acetylated α‐ and β‐isoforms than the mycelium, while the non acetylated α‐isoform was present in greater quantity in the mycelium than in the zoospores or zoosporangia.

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“…Microtubules have been observed in many fungi [1] such as in the aquatic moulds of the genus Allomyces in which they were found to be present in the subapical zone of the vegetative hyphae [2] and in the zoospores [3]. They are known to be involved in flagellar motility [4][5][6], chromosome segregation [7], maintenance of cellular morphology [8] and intracellular transport of organelles including mitochondria and vesicles [9].…”

Section: Introductionmentioning

confidence: 99%

Evolution of tubulin isoforms during the sporophytic development of Allomyces arbuscula

SCHONENBERGER

¹

,

Turian

²

1993

FEMS Microbiology Letters

0

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Tubulins extracted from the sporophytic developmental stages of Allomyces arbuscula have been characterised by one‐ and two‐dimensional SDS‐PAGE gels immunoblotted with monoclonal antibodies as α‐, acetylated α‐ (Mr 57 kDa both) and β‐ (Mr 55 kDa) isoforms. The zoosporangial isoforms could be characterised only when precautions were taken to inhibit the strong tubulin proteolytic activity at this stage. The zoospores and zoosporangia contained greater amounts of the acetylated α‐ and β‐isoforms than the mycelium, while the non acetylated α‐isoform was present in greater quantity in the mycelium than in the zoospores or zoosporangia.

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Analysis of cytoplasmic microtubules and flagellar roots in the zoospores ofAllomyces macrogynus

Cited by 6 publications

References 52 publications

Blastocladiomycota

Blastocladiomycota

Evolution of tubulin isoforms during the sporophytic development of Allomyces arbuscula

Evolution of tubulin isoforms during the sporophytic development of Allomyces arbuscula

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