Analysis of cis–trans isomers and enantiomers of sertraline by cyclodextrin-modified micellar electrokinetic chromatography

Lucangioli, Silvia; Hermida, Laura G.; Trípodi, Valeria; Rodríguez, V.; López, E.E.; Rouge, Pablo D.; Carducci, C. N.

doi:10.1016/s0021-9673(99)00881-x

Cited by 42 publications

(17 citation statements)

References 25 publications

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“…However, while no electrodriven methods are available for the enantioseparation of paroxetine, some have been reported for SRT. The first paper on this subject [37] reports that the separation of SRTand isomers can be obtained by MEKC using a mixture of charged (sulfated) and neutral (hydroxypropyl) b-CD, dissolved in a BGE composed of a pH 9.0 borate buffer and sodium cholate (as the surfactant).…”

Section: Sertralinementioning

confidence: 99%

Advances in the enantioseparation of second‐generation antidepressant drugs by electrodriven methods

Mandrioli

Raggi

2006

Electrophoresis

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Stereochemistry is steadily increasing in importance in the development of new drugs, and the availability of pure enantiomer drugs can make therapy safer and more efficacious. In particular, almost all second-generation antidepressant drugs possess one or more chiral centres; however, only some of them are administered as single enantiomers. A fundamental part of the quality control of pharmaceutical formulations is the determination of enantiomeric excess and enantiomeric purity; this is also important for the therapeutic drug monitoring of depressed patients. For this purpose, efficient and reliable analytical methods are needed and electrodriven techniques (most of all CE, CEC and MEKC) are very efficient and inexpensive candidates for the role. In this review, the enantioselective electrodriven methods available for the analysis of second-generation antidepressant are presented and discussed. In particular, the following pharmacological classes of antidepressants will be considered: selective serotonin reuptake inhibitors (fluoxetine, citalopram, paroxetine, sertraline); norepinephrine reuptake inhibitors (reboxetine); serotonin and norepinephrine reuptake inhibitors (venlafaxine, milnacipran, duloxetine); and noradrenergic and specific serotonergic antidepressants (mirtazapine).

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Section: Sertralinementioning

confidence: 99%

Advances in the enantioseparation of second‐generation antidepressant drugs by electrodriven methods

Mandrioli

Raggi

2006

Electrophoresis

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“…We also report the optimization of experimental conditions for the enantiomeric separation of all the compounds using negatively charged highly sulfated-CDs which have been shown to be particularly effective as single system [17][18][19][20][21][22] or as dual system [23] for the enantiomeric separation of cationic compounds. To the best of our knowledge, no article dealing with chiral separation of such b-carboline derivatives neither in HPLC nor in CE was reported.…”

Section: Introductionmentioning

confidence: 99%

Analytical and Preparative Chiral Separation of β-Carboline Derivatives, LDL Oxidation Inhibitors, Using HPLC and CE Methodologies: Determination of Enantiomeric Purity

et al. 2012

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In the present study, baseline separation of the enantiomers of 16 b-carboline derivatives was successfully achieved using both capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) techniques in short run times (\15 min) and thus permit the determination of enantiomeric excess. In HPLC methodology, cellulose chiral stationary phase (Chiralcel OD-H) was used with a binary mobile phase constituted of n-hexane/ethanol 85/15 leading to a resolution factor of 12.6 in 15 min. Preparative HPLC allowed to obtain pure enantiomers of two compounds. In CE, chiral selectivity was developed with an in-capillary stacking strategy using anionic (highly sulfated-c) cyclodextrins 5% (w/v) as chiral selectors and a 60 mM phosphate buffer (pH 2.5) resulting in a resolution of 10.26 in 14 min of analysis. The analytical characteristics of the two developed methods were studied in terms of repeatability, limits of detection and limits of quantification showing their suitability to be extended to all the other molecules.

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“…CE is efficient, versatile and economical, but it suffers from a lack of sensitivity with regards to therapeutic drug monitoring [14].…”

Section: Introductionmentioning

confidence: 99%

LC–MS Analysis of Sertraline and Its Active Metabolite in Human Serum Using a Silica Column with a Non-Aqueous Polar Mobile Phase

et al. 2011

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A sensitive liquid chromatography-mass spectrometry method for the simultaneous determination of sertraline (SER) and its major metabolite norsertraline (NOR) from serum was developed and validated in the context of a pharmacokinetic study in pregnant women. The separations were achieved on a silica column with a non-aqueous polar mobile phase consisting of acetonitrile, methanol and ammonium acetate at a flow rate of 0.5 mL min -1 . The concentrations were measured using a single quadruple mass spectroscopic detector supplied with atmospheric pressure ionization electrospray. Sample preparation consisted of a simple liquid-liquid procedure. The detector was set in selective ion mode for each compound of interest, 306 m/z for SER and 275 m/z for NOR. Calibration curves were generated by least square linear regression for concentration of 5-160 ng mL -1 for SER and from 10 to 320 ng mL -1 for NOR. The curves for both compounds of interest were linear, with correlation coefficients r 2 C 0.999.

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Analysis of cis–trans isomers and enantiomers of sertraline by cyclodextrin-modified micellar electrokinetic chromatography

Cited by 42 publications

References 25 publications

Advances in the enantioseparation of second‐generation antidepressant drugs by electrodriven methods

Advances in the enantioseparation of second‐generation antidepressant drugs by electrodriven methods

Analytical and Preparative Chiral Separation of β-Carboline Derivatives, LDL Oxidation Inhibitors, Using HPLC and CE Methodologies: Determination of Enantiomeric Purity

LC–MS Analysis of Sertraline and Its Active Metabolite in Human Serum Using a Silica Column with a Non-Aqueous Polar Mobile Phase

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