LC–MS Analysis of Sertraline and Its Active Metabolite in Human Serum Using a Silica Column with a Non-Aqueous Polar Mobile Phase

Fried, Karen; Nolan, Paul E.; Anthony, Marietta; Woosley, Raymond L.; Freeman, Marlene P.

doi:10.1007/s10337-011-1919-4

Cited by 7 publications

(2 citation statements)

References 24 publications

(39 reference statements)

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“…Soukup and Jandera obtained increased retention of phenolic acids with a MeOH–ACN compared to a W–ACN MP but on a silica hydride SP where up to 95% of the silica surface OH groups have been replaced by H atoms. With very few applications published, − empirical data on retention in NA-HILIC are sparse. From a theoretical viewpoint, a retentive principle is difficult to envision for NA-HILIC considering that a W-rich layer cannot form under the experimental conditions.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Aqueous and Nonaqueous Binary Solvent Mixtures as Mobile Phase Alternatives to Water–Acetonitrile Mixtures for Hydrophilic Interaction Liquid Chromatography by Molecular Dynamics Simulations

et al. 2014

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The retentive principle of hydrophilic interaction liquid chromatography (HILIC) with a water (W)−acetonitrile (ACN) mobile phase (MP) and a hydrophilic stationary phase is the formation of a W-rich layer with a rigid and diffuse part in the immediate and extended surface region, respectively. Through molecular dynamics simulations of the adsorption of W−acetone (Ace), W−methanol (MeOH), and MeOH−ACN mixtures to a hydrophilic silica surface, we evaluate their MP potential for aqueous and nonaqueous HILIC. We analyze solvent and hydrogen-bond density profiles, solvent−surface coordination, as well as local solvent orientation, mobility, and composition. Our data show that W−Ace mixtures closely mimic the behavior of W− ACN mixtures, whereas W−MeOH mixtures fail as HILIC MP because the similar affinity of the silica surface for W and MeOH prevents preferential adsorption of W. MeOH−ACN mixtures form a rigid MeOH layer that reverses the surface polarity and prohibits formation of a diffuse MeOH layer in the extended surface region. Generally, a rigid layer at a hydrophilic surface is formed by binary mixtures whose solvents have sufficiently different hydrogen-bonding abilities, and a diffuse layer is formed when the rigid layer maintains hydrophilic properties.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Aqueous and Nonaqueous Binary Solvent Mixtures as Mobile Phase Alternatives to Water–Acetonitrile Mixtures for Hydrophilic Interaction Liquid Chromatography by Molecular Dynamics Simulations

et al. 2014

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“…Chromatographic techniques, such as gas chromatography (GC), [1][2][3] high-performance liquid chromatography (HPLC), [4][5][6][7][8][9][10] and capillary electrophoresis (CE) [11][12][13] have been widely employed for the analysis of SSRIs in environmental and biological samples. Among them, CE offers powerful separation efficiency, rapid analysis and minimal reagent consumption.…”

Section: Introductionmentioning

confidence: 99%

Hollow fiber based liquid–liquid–liquid microextraction combined with sweeping micellar electrokinetic chromatography for the sensitive determination of second-generation antidepressants in human fluids

Zhou

Chen

et al. 2015

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An effective dual preconcentration method involving off-line hollow fiber liquid-liquid-liquid microextraction (HF-LLLME) and on-line sweeping micellar electrokinetic chromatography (sweeping-MEKC) was proposed for the determination of five second-generation antidepressants, including fluoxetine, sertraline, paroxetine, fluvoxamine and citalopram. In HF-LLLME, the analytes were extracted from the sample solution into phenetole impregnated in the pores of the hollow fiber and then back-extracted into 10 μL 0.1 mol L(-1) HAc inside the hollow fiber. Then, the acceptor phase was spiked with 2.8 μL isopropanol (IPA) and introduced into CE for sweeping. In the sweeping-MEKC process, five target analytes were separated in less than 15 min with a background electrolyte consisting of 76% (v/v) 50 mmol L(-1) citric acid (pH 2.2) containing 100 mmol L(-1) sodium dodecyl sulfate (SDS) and 24% (v/v) IPA. The hydrodynamic injection was performed at 50 mbar for 140 s. Under optimized conditions, the limits of detection were in the range of 0.40-1.55 μg L(-1) with enrichment factors of 1897- to 5952-fold for target analytes, with a dynamic linear range of 0.6/5.0-200 μg L(-1). The developed method demonstrated excellent clean-up ability and high enrichment factors and was successfully applied to the analysis of target analytes in human urine and plasma samples.

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A simple and sensitive LC-MS/MS method for the quantitation of sertraline and N-desmethylsertraline in human plasma

Phogole,

Hastie,

Kellermann

2023

Journal of Chromatography B

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LC–MS Analysis of Sertraline and Its Active Metabolite in Human Serum Using a Silica Column with a Non-Aqueous Polar Mobile Phase

Cited by 7 publications

References 24 publications

Evaluation of Aqueous and Nonaqueous Binary Solvent Mixtures as Mobile Phase Alternatives to Water–Acetonitrile Mixtures for Hydrophilic Interaction Liquid Chromatography by Molecular Dynamics Simulations

Evaluation of Aqueous and Nonaqueous Binary Solvent Mixtures as Mobile Phase Alternatives to Water–Acetonitrile Mixtures for Hydrophilic Interaction Liquid Chromatography by Molecular Dynamics Simulations

Hollow fiber based liquid–liquid–liquid microextraction combined with sweeping micellar electrokinetic chromatography for the sensitive determination of second-generation antidepressants in human fluids

A simple and sensitive LC-MS/MS method for the quantitation of sertraline and N-desmethylsertraline in human plasma

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