Analysis of chitosan by gel permeation chromatography

Zielińska, Katarzyna; Шостенко, А. Г.; Truszkowski, S.

doi:10.1134/s0018143914020143

Cited by 12 publications

(10 citation statements)

References 8 publications

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“…Molecular weight [21,22] Gel permeation chromatography [23][24][25] From a chemical viewpoint, chitin is a poly(β-(1-4)-N-acetyl-d-glucosamine) with β(1→4) linkages [1], and it is considered to have a cellulose-like structure, where the hydroxyl group in the C2 position has been replaced by an acetamido group (Figure 1). Depending on the orientation of polysaccharide chains, chitin displays three polymorphs, denoted as α, β and γ [3].…”

Section: Viscosimetrymentioning

confidence: 99%

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Chitosan: A Natural Biopolymer with a Wide and Varied Range of Applications

2020

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Although chitin is of the most available biopolymers on Earth its uses and applications are limited due to its low solubility. The deacetylation of chitin leads to chitosan. This biopolymer, composed of randomly distributed β-(1-4)-linked D-units, has better physicochemical properties due to the facts that it is possible to dissolve this biopolymer under acidic conditions, it can adopt several conformations or structures and it can be functionalized with a wide range of functional groups to modulate its superficial composition to a specific application. Chitosan is considered a highly biocompatible biopolymer due to its biodegradability, bioadhesivity and bioactivity in such a way this biopolymer displays a wide range of applications. Thus, chitosan is a promising biopolymer for numerous applications in the biomedical field (skin, bone, tissue engineering, artificial kidneys, nerves, livers, wound healing). This biopolymer is also employed to trap both organic compounds and dyes or for the selective separation of binary mixtures. In addition, chitosan can also be used as catalyst or can be used as starting molecule to obtain high added value products. Considering these premises, this review is focused on the structure and modification of chitosan as well as its uses and applications.

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Section: Viscosimetrymentioning

confidence: 99%

“…Deacetylation degree [12,13] Elemental analysis [14] Fourier transform infrared (FTIR) [15][16][17][18] Nuclear magnetic resonance (NMR) [19,20] Viscosimetry Molecular weight [21,22] Gel permeation chromatography [23][24][25] Molecules 2020, 25…”

Section: Potenciometric Titrationmentioning

confidence: 99%

Chitosan: A Natural Biopolymer with a Wide and Varied Range of Applications

2020

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“…Previously, M n determination of chitosan compounds has been reported using PL aquagel−OH mixed (300 mm) column, aqueous acetic acid/sodium acetate eluent, and noncharged poly(ethylene oxide)/poly(ethylene glycol) standards of 1970−1345000 g/mol. 36 In the present work, similar type of column and standards were used, and aqueous sodium sulfate eluent was employed to minimize the interaction between the charged polymers and the column. The use of noncharged standards and vinyl-based stationary phase to characterize charged polymers imposed a limitation in the M n determination.…”

Section: T H I S C O N T E N T Imentioning

confidence: 99%

Chitosan-Based Peptidopolysaccharides as Cationic Antimicrobial Agents and Antibacterial Coatings

et al. 2018

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The rapid spread of multidrug-resistant bacteria has called for effective antimicrobial agents which work on a more direct mechanism of killing. Cationic peptidopolysaccharides are developed in the present work to mimic the peptidoglycan structure of bacteria and to enhance the membrane-compromising bactericidal efficacy. Antimicrobial CysHHC10 peptide was grafted to the C-2 (amino) or C-6 (hydroxyl) position of chitosan backbone via thiol-maleimide "click" conjugation, utilizing the maleimidohexanoic linkers. The peptidopolysaccharide with primary amino backbone intact (CSOHHC) exhibited higher bactericidal activity toward Gram-positive and Gram-negative bacteria, in comparison to that with amino backbone grafted with the peptide (CSNHHC). Both peptidopolysaccharides also exhibited lower hemolytic activity and cytotoxicity than free CysHHC10 peptide due to the moderation effect contributed by the chitosan backbone. For targeting the Gram-positive bacteria in particular, the CSOHHC expressed 4- and 2-fold increases in hemo- and cytoselectivity, respectively, as compared to the CysHHC10 peptide. In an extended application, peptidopolysaccharide antibacterial coatings were formed via layer-by-layer assembly with tannic acid. The peptidopolysaccharide coatings readily killed the adhered bacteria upon contact while being cytocompatible by maintaining more than 60% viability for the adhered fibroblasts. Therefore, the peptidoglycan-mimetic peptidopolysaccharides are potential candidates for anti-infective drugs in biomedical applications.

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“…Deacetylation degree (DD) Potentiometric titration [27,28] Elemental analysis [29] Fourier transform infrared (FTIR) [30][31][32][33] Nuclear magnetic resonance (NMR) [34][35][36] Molecular weight (MW) Viscometry [37,38] Gel permeation chromatography (GPC) [37,39,40] Biological Activities and Application of Marine Polysaccharidesglutaric acid. The cross-linking of chitosan with glutaraldehyde and sulfosuccinic acid has been used to generate membranes with a good proton conductivity, which makes them useful in fuel cells [44].…”

Section: Chitosan Property Characterization Methodsmentioning

confidence: 99%

Chitosan, Chitosan Derivatives and their Biomedical Applications

Muñoz¹,

Corrales²

2017

Biological Activities and Application of Marine Polysaccharides

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Chitosan is one of the most studied polysaccharides nowadays. Because of its biocompatibility, biodegradability and abundance in nature, it has had a wide number of applications. In this chapter, an overview of chitosan including its physicochemical properties and characterization methods is presented. Subsequently, the main chitosan chemical modiications via the hydroxyl and amino groups are discussed. These chemical modiications improve chitosan physical properties and expand its range of applications especially in the biomedical ield which will also be studied.

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Analysis of chitosan by gel permeation chromatography

Cited by 12 publications

References 8 publications

Chitosan: A Natural Biopolymer with a Wide and Varied Range of Applications

Chitosan: A Natural Biopolymer with a Wide and Varied Range of Applications

Chitosan-Based Peptidopolysaccharides as Cationic Antimicrobial Agents and Antibacterial Coatings

Chitosan, Chitosan Derivatives and their Biomedical Applications

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