Analysis of chemical composition and its analgesic and anti-inflammatory activity of essential oil of sintoc bark (Cinnamomum sintoc bl.) using in vivo methods

Abstract

Sintoc bark (Cinnamomum sintoc Bl) belongs to Lauraceae (the laurel family). It has been used empirically for a treatment for swelling caused by insects' bites. In this study, the research examined the analgesic activity and anti-inflammation of essential oil of sintocbark using in vivo methods. The mechanism of anti-inflammation was predicted using molecular docking against COX-2. Essential oil of sintoc bark was collected by distilling through steam distillation, and then analyzed by GC-MS. Analgesic and anti-inflammatory activity was examined by in vivo, which were conducted by writhing and carrageenan-induced methods, respectively. The findings showed that the tested sintoc bark oils contained 36 components of essential oil with eugenol (38.38 %) as a major compound. In the in vivo experiments, sintoc bark oils with doses 0.005 mL, 0.010 mL, and 0.020 mL/20g body weight significantly (p<0.05) reduced the number of writhing of mice when compared to negative control group. All of doses of sintoc bark oils gave significantly affect (confidence level 99 %) compare to negative control. Sintoc oil with dose 0.2 ml/200g had the strongest inhibition compare to positive control (indometasin 10 mg/kg body weight). The molecular docking results indicated that the compounds of aryl propanoid were generally potential to inhibit COX-2.

Antinociceptive Activity of Chemical Components of Essential Oils That Involves Docking Studies: A Review

“…The most prominent plant families used in the studies identified were Asteraceae, (Garcıá et al, 2011;Radulovićet al, 2015;Leite et al, 2019); Euphorbiaceae, de Oliveira junior et al, 2018); Verbenaceae, (Siqueira-Lima et al, 2017); Lamiaceae, (Quintans-Junior et al, 2018), and Lauraceae (Sumiwi et al, 2015).…”

“…Of the species, Croton conduplicatus Kunth prevailed de Oliveira junior et al, 2018); with Vanillosmopsis arborea Baker (Leite et al, 2019); then Achillea falcata L.; (Radulovićet al, 2015); Ageratin glabrata; (Garcıá et al, 2011); Hyptis pectinata; (Quintans-Junior et al, 2018); Cinnamomum; sintoc bl. ; (Sumiwi et al, 2015); and Grateful Lippia Schauer (Siqueira-Lima et al, 2017).…”

Pharmacological properties and their medicinal uses ofCinnamomum: a review

“…[249] Terpinenes: Hydrocarbons: a-Cadinene; c-cadinene; d-cadinene. [250] Terpinenes: 1,8-Cineole; caryophyllene oxide; germacrene-D; limonene; linalool; myrcene; pcymene; terpinen-4-ol; trans-linalool oxide; a-cadinol; a-humulene; a-terpineol; b-caryophyllene; b-elemene; b-pinene; c-elemene; c-muurolene; c-terpinene [250] Analgesic, [251] anti-inflammatory, [251] antimicrobial, [252] insecticidal, [203] platelet-activating factor (PAF) receptor-binding antagonist activity [39] Abdominal pain, [253] animal bites, [254] diarrhoea, [253] dysentery, [251] fatigue, [115] flatulence, [252] epilepsy, [254] intestinal complaints, [253] insects bite, [253] inflammation, [251] mouth freshener, [253] numbness, [253] pyrexia, [115,252] rheumatism, [254] snake bite, [253] swelling, [254] syphilis, [253] tiredness, [115] ulcer [ [251] Aldehyde: Dodecanal; tetradecanal; undecanal. [250] Fatty acid: Tetradecanoic acid.…”

Cinnamomum sintoc Blume Lauraceae

“…Cinnamomum sintoc bark contains 1.1% essential which is proven to have antiinflammatory properties (Sumiwi et al 2015). The secondary metabolite compounds in C. sintok leaves were flavonoids, alkaloids, terpenoids steroids, and tannins.…”