Analysis of cashmere goat meat by label-free proteomics shows that MYL3 is a potential molecular marker of meat toughness

Xie, Yingxin; Nai, Rile; Li, Xuewu; Li, Haijun; Zhao, Meng; Che, Tianyu; Cai, Ting; Liu, Zhihong; Li, Jinquan

doi:10.17221/61/2021-cjas

Cited by 4 publications

(3 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The procedure was performed basically according to Xie et al (2022). To the samples of protein solution (see above), containing 100 µg of protein, 200 µl of 8 M urea and 10 mM dl-dithiothreitol were added and incubated at 37 °C for 1 hour.…”

Section: Tryptic Digestion Of Proteinsmentioning

confidence: 99%

“…STRING (https://cn.string-db.org/) was used to perform the protein-protein interaction (PPI) analysis. SWISS-MODEL (https://swissmodel.expasy.org/) was used to predict the three-dimensional structure of the proteins (Xie et al 2022).…”

Section: Bioinformatics Analysismentioning

confidence: 99%

“…The source proteins obtained above were boiled at 100 °C for 3 min to become denatured proteins. The procedure was referenced according to Xie et al (2022). The denatured proteins were separated using SDS-PAGE and transferred to PVDF membranes (Bio-Rad, Lincoln, NE, USA).…”

Section: Western Blottingmentioning

confidence: 99%

See 2 more Smart Citations

Label-free proteomics to identify keratins and keratin-associated proteins and their effects on the fleece traits of Inner Mongolia Cashmere Goats

Zhang¹,

Xie²,

Guo³

et al. 2023

CZECH J ANIM SCI

Self Cite

View full text Add to dashboard Cite

Inner Mongolia Cashmere Goat fleece is derived from a heterogeneous coat. The guard hair is produced by primary hair follicles, and the down hair is produced by secondary hair follicles. Reports have focused on research related to the different hair follicle types, but no related study has addressed the difference in the proteins of down hair (DH) and guard hair (GH), and whether the protein composition of these materials differs remains to be studied. The protein composition of DH and GH was studied. A total of 108 proteins were identified in DH, and 116 proteins were identified in GH; 39 proteins were differentially expressed, of which 18 proteins were upregulated and 21 proteins were downregulated in DH versus GH. In this study, through the measurement of DH and GH samples from Inner Mongolia Cashmere Goats, the diameter of DH relative to GH was downregulated (P < 0.05), a property that may be related to the KRT38 protein. Moreover, the strength of GH was significantly higher than that of DH (P < 0.05), a property that may be affected by the KRTAP8-1 protein. GO analysis showed that the different traits of DH and GH from Inner Mongolia Cashmere Goats are affected by keratin, actin, and calcium-binding proteins. This study uses a nonlabelled quantitative proteomics method to study the proteins in DH and GH, aiming to identify the keratin family in Inner Mongolia Cashmere Goats and to provide a new direction for studying DH and GH traits at the protein level.

show abstract

Section: Tryptic Digestion Of Proteinsmentioning

confidence: 99%

Section: Bioinformatics Analysismentioning

confidence: 99%