Analysis of carbamazepine serum by differential pulse voltammetry (DPV) and comparison with fluorescence polarization immunoassay (FPIA): an animal study

Lin, Wen-Dar; Pan, Min; Wang, H. Y.; Su, Yuhlong Oliver; Huang, Pengfei

doi:10.1007/s00044-012-9981-5

Cited by 17 publications

(5 citation statements)

References 28 publications

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“…A two-wave oxidation peak has been observed accompanied by a small reduction peak, which have suggested an oxidation pathway with formation of dimmers similar to the one proposed in [13]. Lin et al have used differential pulse voltametry to determine the serum level of CBZ in rabbits and reported a detection limit of 59.2×10 -8 mol dm -3 [17]. Excellent enhancement effects on electrochemical response of CBZ have been achieved through the use of fullerene-C 60 modified GCE [18].…”

mentioning

confidence: 65%

“…Due to its low water solubility (∼72×10 -5 mol dm -3 at 25 °C), the electrochemical characterization of CBZ has been mostly performed with addition of organic solvents such as acetonitrile and dimethylformamide [14][15][16][17]. However, these solvents interact with the surface of gold electrode and easily adsorb on it [23], which makes them inconvenient for the purposes of the present research.…”

Section: Resultsmentioning

confidence: 99%

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Electrochemical characterization and determination of carbamazepine as pharmaceutical standard and tablet content on gold electrode

Trišović¹,

Božić²,

Petrović³

et al. 2014

Hem Ind

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The anodic behaviour of carbamazepine (CBZ), an anticonvulsant drug, has been studied on gold electrode in 0.1 mol dm-3 phosphate buffer of pH 7.0 by using cyclic voltammetry. It has been found that the value of the oxidative current of pure CBZ at +0.90 V is a linear function of the concentration in a range from 1.0?10-7 to 1.0?10?4 mol dm?3. The detection of CBZ in the concentration of 1.0?10-8 mol dm-3 is among the lowest that have been reported for this drug using voltammetric techniques. CBZ as a content of tablet Galepsine? has been quantitatively determined. It has also been demonstrated that the modification of gold electrode with bovine serum albumin (BSA) results in a decrease of the oxidative peak current due to the binding of the drug to BSA. [Projekat Ministarstva nauke Republike Srbije, br. 172013]

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confidence: 65%

Section: Resultsmentioning

confidence: 99%

Electrochemical characterization and determination of carbamazepine as pharmaceutical standard and tablet content on gold electrode

Trišović¹,

Božić²,

Petrović³

et al. 2014

Hem Ind

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“…These methods require time-consuming pretreatment processes and sophisticated instrumentation with high per-assay costs, relegating them to centralized laboratory-based tests. , Such methods cannot be applied for rapid, point-of-care therapeutic drug monitoring. Several attempts at portable assay formats using electrochemical techniques to rapidly detect CBZ and its metabolites have been proposed to overcome the aforementioned limitations. , Direct electrochemical methods rely on target reduction/oxidation (redox) without an affinity reagent for quantification. ,− Such direct electrochemical methods are simple, easy, and fast; however, the high oxidation (>0.7 V) and low reduction (<−2.0 V) potentials of CBZ , lead to significant false positives from other electroactive small molecules (i.e., ascorbic acid, uric acid, dopamine, oxygen, etc.) present at high concentrations in serum.…”

Section: Introductionmentioning

confidence: 99%

“…Several attempts at portable assay formats using electrochemical techniques to rapidly detect CBZ and its metabolites have been proposed to overcome the aforementioned limitations. 20 , 21 Direct electrochemical methods rely on target reduction/oxidation (redox) without an affinity reagent for quantification. 3 , 22 − 25 Such direct electrochemical methods are simple, easy, and fast; however, the high oxidation (>0.7 V) and low reduction (<−2.0 V) potentials of CBZ 24 , 25 lead to significant false positives from other electroactive small molecules (i.e., ascorbic acid, uric acid, dopamine, oxygen, etc.)…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Carbamazepine Aptasensor for Therapeutic Drug Monitoring at the Point of Care

Chung

Singh

Gribkoff³

et al. 2022

ACS Omega

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Monitoring the anti-epileptic drug carbamazepine (CBZ) is crucial for proper dosing, optimizing a patient’s clinical outcome, and managing their medication regimen. Due to its narrow therapeutic window and concentration-related toxicity, CBZ is prescribed and monitored in a highly personalized manner. We report an electrochemical conformation-changing aptasensor with two assay formats: a 30 min assay for routine monitoring and a 5 min assay for rapid emergency testing. To enable “sample-to-answer” testing, a de novo CBZ aptamer (K d < 12 nM) with conformational switching due to a G-quadruplex motif was labeled with methylene blue and immobilized on a gold electrode. The electrode fabrication and detection conditions were optimized using electrochemical techniques and visualized by atomic force microscopy (AFM). The aptasensor performance, including reproducibility, stability, and interference, was characterized using electrochemical impedance spectroscopy and voltammetry techniques. The aptasensor exhibited a wide dynamic range in buffer (10 nM to 100 μM) with limits of detection of 1.25 and 1.82 nM for the 5 and 30 min assays, respectively. The clinical applicability is demonstrated by detecting CBZ in finger prick blood samples (<50 μL). The proposed assays provide a promising method to enable point-of-care monitoring for timely personalized CBZ dosing.

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“…Several analytical techniques have been developed for CBZ sensing including fluorescence probing (Ma et al, 2018), liquid chromatography (Yan and Row, 2006), and electrochemical methods such as cyclic voltammetry (Veiga et al, 2010), differential pulse voltammetry (DPV) (Lin et al, 2012), amperometric techniques (De Carlo et al, 2015), enzyme modified immunoassay, and so on (Contin et al, 1985). However, most of these analytical techniques involve complicated sample preparation steps, resulting in unspecified impurities, and the sample disregard limit is high, thus resulting in CBZ waste generation (Trišović et al, 2014) in the test solutions.…”

Section: Introductionmentioning

confidence: 99%

Two-Dimensional Metallic NiSe2 Nanoclusters–Based Low-Cost, Flexible, Amperometric Sensor for Detection of Neurological Drug Carbamazepine in Human Sweat Samples

Veeralingam

Badhulika

2020

Front. Chem.

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Here we report a low-cost, flexible amperometric sensing platform for highly selective and sensitive detection of carbamazepine (CBZ) in human sweat samples. Detailed morphological characterization of the two-dimensional transition metal dichalcogenide NiSe 2 , synthesized using one-step hydrothermal method, confirms the formation of dense NiSe 2 nanoclusters in the range of 500-650 nm, whereas X-ray diffraction and X-ray photoelectron spectroscopy studies reveal a stable and pure cubic crystalline phase of NiSe 2. The sensor device is fabricated by uniformly depositing an optimized weight percentage of as-synthesized NiSe 2 onto flexible and biocompatible polyimide substrate using spin coating, and metal contacts are established using thermal evaporation technique. The sensor exhibits a remarkable sensitivity of 65.65 µA/nM over a wide linear range of 50 nM to 10 µM CBZ concentrations and a low limit of detection of 18.2 nM. The sensing mechanism and excellent response of NiSe 2 toward CBZ can be attributed to the highly conductive metallic NiSe 2 , large electroactive surface area of its nanoclusters, and highly interactive Ni 2+ /Ni 3+ oxidation states. Furthermore, the presence of 10-fold excess of capable interferents, such as lactic acid, glucose, uric acid, and ascorbic acid, does not affect the accurate determination of CBZ, thus demonstrating excellent selectivity. The real-time detection of CBZ is evaluated in human sweat samples using standard addition method, which yields reliable results. Furthermore, the sensor shows excellent robustness when subject to bending cycles and fast response time of 2 s. The strategy outlined here is useful in developing sensing platforms at low potential without the use of enzymes or redox binders for applications in healthcare.

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Analysis of carbamazepine serum by differential pulse voltammetry (DPV) and comparison with fluorescence polarization immunoassay (FPIA): an animal study

Cited by 17 publications

References 28 publications

Electrochemical characterization and determination of carbamazepine as pharmaceutical standard and tablet content on gold electrode

Electrochemical characterization and determination of carbamazepine as pharmaceutical standard and tablet content on gold electrode

Electrochemical Carbamazepine Aptasensor for Therapeutic Drug Monitoring at the Point of Care

Two-Dimensional Metallic NiSe2 Nanoclusters–Based Low-Cost, Flexible, Amperometric Sensor for Detection of Neurological Drug Carbamazepine in Human Sweat Samples

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