Analysis of age‐associated alternation of SCSA sperm DNA fragmentation index and semen characteristics of 1790 subfertile males in China

Lu, Rui-Jing; Chen, Xian; Wang, Yu; Fan, Jun; Zhou, Xiyou; Xu, Yang; Wang, Feng

doi:10.1002/jcla.23548

Cited by 16 publications

(10 citation statements)

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“…Applying the statistical method, the only score related to aging is sperm necrosis. Sperm necrosis is characterised by morphological features as disrupted chromatin, swollen mitochondria and broken plasma membrane, and the link between sperm necrosis and aging is in line with the increase of DNA fragmentation in advanced paternal age described by other authors 39 . Many studies reported a positive correlation between increasing male age and sperm DNA damage 40,41 doubling from 25 to 55 years of age 42 .…”

Section: Discussionsupporting

confidence: 76%

Influence of age on sperm characteristics evaluated by light and electron microscopies

Collodel

Ferretti

Masini

et al. 2021

Sci Rep

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The impact of male aging on male fertility has only recently become of interest to the scientific community. This study aims to assess the relationship between age and fertility among a sample of men, considering the individual and pathological characteristics. In this retrospective study data of semen analysis and medical history of 1294 Italian male patients were considered. Semen analysis was performed by light microscopy and transmission electron microscopy mathematically elaborated. A generalized linear model was used to explore the influence of male age on semen quality, considering as confounders wine consumption, smoking habits, presence of varicocele, consanguinity and positive semen bacteriological analysis and urethral swab. The mean age of the participants was 36.41 ± 6.379. Male aging without impact of confounders was correlated with a decrease in sperm concentration and motility and an increased in sperm necrosis. Sperm concentration and progressive motility were negatively related to the presence of confounders as wine consumption (sperm motility), urogenital infection (sperm concentration and motility), varicocele (sperm concentration) and consanguinity (sperm motility). Urogenital infection, varicocele and consanguinity positively correlated with sperm necrosis. The most important finding was the observation of a negative effect of male aging on sperm parameters such as concentration, motility, and viability. It is possible to hypothesize age-dependent changes of testicular environment, probably related to reactive oxygen species production. The demonstration, in a large sample of patients, that aging influences sperm quality strongly motivates further research focused on the mechanisms involved in this phenomenon and its effects on offspring fitness.

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Section: Discussionsupporting

confidence: 76%

Influence of age on sperm characteristics evaluated by light and electron microscopies

Collodel

Ferretti

Masini

et al. 2021

Sci Rep

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“…In the current study, the analysis of 1638 IVF/ICSI cycles showed that the sperm DFI was significantly positively correlated with male age, indicating that the degree of sperm DNA fragmentation increased with age (Supplementary Table 1). This is consistent with the findings of Bellver (15), Ghanbarzadeh (16), Zhang (17), Gonzalez (18), Lu (19) and Belloc (20). The sperm DFI was significantly and positively correlated with abstinence days, semen volume, immotile sperm percentage, sperm high-staining HDS percentage and other parameters, suggesting that the increase in abstinence days may increase semen volume, but the immotile sperm percentage and sperm high-staining HDS percentage may increase at the same time.…”

Section: Discussionsupporting

confidence: 93%

Influence of sperm DNA fragmentation on the clinical outcome of in vitro fertilization-embryo transfer (IVF-ET)

et al. 2022

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PurposeTo evaluate the effect of elevated sperm DNA fragmentation index (DFI) on fresh and frozen embryo transfer cycles.MethodsA retrospective study was performed with 549 fresh embryo transfer cycles and 1340 frozen embryo transfer cycles after in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) from 2016 to 2021.ResultsThe statistical results of 549 fresh embryo transfer cycles showed that the delivery rate in the normal sperm DFI group (43.9% vs. 27.1%, P = 0.014) was significantly higher than that in the abnormal sperm DFI group, and there were no significant differences in the biochemical pregnancy rate (59.0% vs. 50.8%, P = 0.232), clinical pregnancy rate (53.1% vs. 40.7%, P = 0.072), or miscarriage rate (17.3% vs. 33.3%, P = 0.098) between the two groups. The results of 1340 frozen embryo transfer cycles showed that the biochemical pregnancy rate (57.9% vs. 45.6%, P = 0.006) and clinical pregnancy rate (50.3% vs. 40.7%, P = 0.027) in the normal sperm DFI group were significantly higher than those in the abnormal sperm DFI group. The delivery rate (40.9% vs. 33.3%, P = 0.074) and miscarriage rate (18.6% vs. 18.0%, P = 0.919) were not significantly different between the two groups.ConclusionThe increase of sperm DFI significantly reduced the delivery rate of fresh embryo transfer cycles and the biochemical pregnancy rate and clinical pregnancy rate of frozen embryo transfer cycles.

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“…In comparison, the results from a French Study applying the Tunel test which undertook a prospective analysis of 1633 patients [ 31 ] and a study from Shenzhen in China on another large cohort of 1790 patients’ applying a retrospective analysis of the SCSA Test for correlation between DNA damage and sperm parameters [ 32 ]. The majority of their findings were consistent with our study, i.e., both progressive motility and normal morphology were inversely correlated to the rate of DNA damage, with no correlation being found between the DFI and sperm concentration; age, tail defects, and abstinence time were positively correlated with DFI.…”

Section: Discussionmentioning

confidence: 99%

How Well Do Semen Analysis Parameters Correlate with Sperm DNA Fragmentation? A Retrospective Study from 2567 Semen Samples Analyzed by the Halosperm Test

Chua

Yovich

Hinchliffe

et al. 2023

JPM

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Sperm DNA fragmentation (SDF) levels have been measured in the workup for in vitro fertilization (IVF) at PIVET since 2007, with the Halosperm test having replaced the previous sperm chromatin structure assay (SCSA) since 2013. Of 2624 semen samples analyzed for the Halosperm test, 57 were excluded as the sperm concentration was <5 million/mL, a level too low for accurate testing, leaving 2567 samples for assessment within this study. The SDF rates were categorized in 5 sperm DNA fragmentation indices (DFI), ranging from <5% to levels >30%, and these categories were correlated with the respective semen analysis profiles and two clinical parameters, namely the age of the male and the ejaculatory abstinence period prior to the sample. The results showed a significant correlation with male age (r = 0.088; p < 0.0001), the abstinence period (r = 0.076; p = 0.0001), and the semen volume (r 0.063; p = 0.001), meaning an adversely high SDF was associated with advanced age, prolonged abstinence, and raised semen volume parameters. There was a significant negative correlation with sperm morphology (r = −0.074; p = 0.0001), progressive motility (r = −0.257; p < 0.0001), and semen pH (r = −0.066; p < 0.001), meaning these semen anomalies were associated with high SDF values. With respect to abnormal morphology, sperm tail defects had a positive correlation (r = 0.096; p < 0.0001) while midpiece defects showed a negative correlation (r = −0.057; p = 0.004), meaning that tail defects are most likely to associate with adverse DFI values. With respect to motility patterns, the poorer patterns showed a positive correlation with increased DFI, namely C pattern (r = 0.055; p = 0.005) and D pattern (r = 0.253; p < 0.0001). These results imply that raised DFI reflects poor sperm quality and should be investigated in clinical trials involving IVF and the consideration of intracytoplasmic sperm injection (ICSI).

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Analysis of age‐associated alternation of SCSA sperm DNA fragmentation index and semen characteristics of 1790 subfertile males in China

Abstract: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Cited by 16 publications

References 43 publications

Influence of age on sperm characteristics evaluated by light and electron microscopies

Influence of age on sperm characteristics evaluated by light and electron microscopies

Influence of sperm DNA fragmentation on the clinical outcome of in vitro fertilization-embryo transfer (IVF-ET)

How Well Do Semen Analysis Parameters Correlate with Sperm DNA Fragmentation? A Retrospective Study from 2567 Semen Samples Analyzed by the Halosperm Test

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