Influence of sperm DNA fragmentation on the clinical outcome of in vitro fertilization-embryo transfer (IVF-ET)

Zhu, Chun-Hui; Chen, Fang; Zhang, Shengmin; She, Hong; Ju, Yun; Wen, Xidong; Chao, Yang; Sun, Yan; Dong, Naijun; Xue, Tongmin; Liu, Kaifeng; Li, Feng; Cui, Hengmi

doi:10.3389/fendo.2022.945242

Cited by 11 publications

(11 citation statements)

References 30 publications

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“…Previous studies have reported that PRDX4 is signi cantly upregulated in bronchoalveolar lavage uid (BALF) from patients with silicosis at different stages [23]. Moreover, the Prdx4 protein in human follicular uid can serve as an effective factor for predicting the subsequent clinical outcomes of in vitro fertilization-embryo transfer (IVF-ET) patients [24]. This suggests that PRDX4 has the potential to be a predictive biomarker.…”

Section: Discussionmentioning

confidence: 99%

PRDX4 regulates AMs cell activity through the AKT/NF-κB pathway to inhibit the progress of pulmonary fibrosis

Zhou

Liu

et al. 2023

Preprint

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Background Idiopathic pulmonary fibrosis (IPF) is a fatal disease that leads to severe lung fibrosis, and PRDX4 expressed highly in AMs may be closely related to the process of fibrosis progression. This study investigates the function and molecular mechanisms of the potential therapeutic target PRDX4 in idiopathic pulmonary fibrosis (IPF). Methods This study utilized single-cell RNA sequencing (scRNA-seq) of lung tissue, transcriptome sequencing (RNA-Seq) of bronchoalveolar lavage fluid (BALF), and clinical data retrieval to investigate key cells influencing inflammation and fibrosis in patients with idiopathic pulmonary fibrosis (IPF), and to analyze the expression and prognostic value of PRDXs. AAV-mediated knockdown of PRDX4 protein expression was performed specifically in alveolar macrophages (AMs) of IPF mice, and lung function as well as changes in inflammatory and fibrotic gene expression were evaluated. In vitro cell experiments and tissue immunofluorescence were conducted to investigate the regulatory mechanisms of PRDX4 both in vivo and in vitro. Results Alveolar macrophages (AMs) and type II lung epithelial cells (AT2) are the major contributing cells for inflammation and fibrosis genes in lung tissue of IPF patients. High expression of PRDX4 in AMs is significantly negatively correlated with patient prognosis (p < 0.05). Functionally, knockdown of PRDX4 expression in AMs effectively improves lung function (EF50, VT, and MV) in IPF mice, while inhibiting the expression of genes related to lung inflammation and fibrosis. Mechanistically, PRDX4 can regulate the secretion of inflammation-related factors in AMs through the AKT/NF-κB pathway, thereby inhibiting the progression of inflammation and fibrosis in IPF mice. Conclusions Inhibiting the expression of PRDX4 in alveolar macrophages (AMs) can modulate the activity of these cells, thereby suppressing the progression of IPF disease.

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Section: Discussionmentioning

confidence: 99%

PRDX4 regulates AMs cell activity through the AKT/NF-κB pathway to inhibit the progress of pulmonary fibrosis

Zhou

Liu

et al. 2023

Preprint

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“…Although ICSI has been proposed to be effective when any viable sperm are present [2], increasing data has suggested that male factors can have an independent effect on ART outcome. The level of sperm production/cause of azoospermia as well as sperm source [3], sperm DNA fragmentation for embryos used for transfer [4], advanced paternal age [5], male body mass index/ obesity [6] have all been reported to affect ART success. Increasing attention has been paid to the potential role of ejaculatory abstinence on semen quality and IVF success.…”

Section: Introductionmentioning

confidence: 99%

Abstinence for sperm sample collection and ART outcome: an unsubstantiated myth

Schlegel

2024

Current Opinion in Obstetrics &Amp; Gynecology

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Purpose of review Semen analysis is a basic component of male evaluation. Reproductive centers typically instruct men to provide a semen specimen based on recommendations from WHO Standard for semen examination. Evidence that these recommendations optimize sperm reproductive capacity is lacking. Existing data to optimize sperm quality with shorter abstinence were reviewed. Recent findings Several recent studies have reviewed the effects of shorter ejaculatory abstinence of semen quality and assisted reproductive technology (ART) outcomes. Shorter abstinence was defined as 1 h–1 day in one review, and <4 h in the other systematic meta-analysis and review. Summary Prior instructions for male patients have not been designed to optimize fertility potential for semen analyses. Optimal sperm quality is obtained by instructing men to have a short abstinence (certainly <1 day, and preferably <4 h) for semen specimens used for in vitro fertilization (assisted reproduction)

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“…However, the repair capacity of oocytes largely depends on their quality and the degree of sperm DNA fragmentation, and the fraction above the repair threshold may be transferred to the embryo and have genetic significance for chromosomal aneuploidy, especially in advanced women (≥ 35 years old) ( 13 – 15 ). Accumulating evidence indicates that high sperm DNA damage directly affects male infertility, which is closely related to poor embryo quality, low pregnancy rate and high miscarriage rate ( 16 – 19 ). A high sperm DNA fragmentation (SDF) index seems to reflect high chromosome abnormalities to a certain extent, thus increasing the risk of aneuploid embryos ( 5 , 20 , 21 ).…”

Section: Introductionmentioning

confidence: 99%

Elevated sperm DNA fragmentation is correlated with an increased chromosomal aneuploidy rate of miscarried conceptus in women of advanced age undergoing fresh embryo transfer cycle

Fu,

Cui,

et al. 2024

Front. Endocrinol.

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BackgroundMale sperm DNA fragmentation (SDF) may be associated with assisted reproductive technology (ART) outcomes, but the impact of SDF on the occurrence of aneuploid-related miscarriage remains controversial.MethodsGenome-wide single-nucleotide polymorphism-based chromosomal microarray analysis was performed on 495 miscarried chorionic villus samples undergone IVF/ICSI treatment from the Reproductive Medicine Center of the First Affiliated Hospital of Zhengzhou University. SDF was assessed using sperm chromatin structure assay. Patients were divided into four groups according to embryo transfer cycle type and maternal age, and the correlation between SDF and chromosome aberration was analyzed. A receiver operating characteristic (ROC) curve was utilized to find the optimal threshold.ResultsTotal chromosomal aneuploidy rate was 54.95%, and trisomy was the most common abnormality (71.32%). The chromosomally abnormal group had higher SDF than the normal group (11.42% [6.82%, 16.54%] vs. 12.95% [9.61%, 20.58%], P = 0.032). After grouping, elevated SDF was significantly correlated with an increasing chromosome aneuploidy rate only in women of advanced age who underwent fresh embryo transfer (adjusted odds ratio:1.14 [1.00–1.29], adjusted-P = 0.045). The receiver operating characteristic curve showed that SDF can predict the occurrence of chromosomal abnormality of miscarried conceptus in this group ((area under the curve = 0.76 [0.60–0.91], P = 0.005), and 8.5% was the optimum threshold. When SDF was ≥ 8.5%, the risk of such patients increased by 5.76 times (adjusted odds ratio: 6.76 [1.20–37.99], adjusted-P = 0.030).ConclusionFor women of advanced maternal age undergoing fresh embryo transfer, older oocytes fertilized using sperm with high SDF in IVF/ICSI treatment might increase the risk of chromosomal abnormality in miscarried conceptus.

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Influence of sperm DNA fragmentation on the clinical outcome of in vitro fertilization-embryo transfer (IVF-ET)

Cited by 11 publications

References 30 publications

PRDX4 regulates AMs cell activity through the AKT/NF-κB pathway to inhibit the progress of pulmonary fibrosis

PRDX4 regulates AMs cell activity through the AKT/NF-κB pathway to inhibit the progress of pulmonary fibrosis

Abstinence for sperm sample collection and ART outcome: an unsubstantiated myth

Elevated sperm DNA fragmentation is correlated with an increased chromosomal aneuploidy rate of miscarried conceptus in women of advanced age undergoing fresh embryo transfer cycle

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