Analysis and measurement of serotonin

Szeitz, András; Bandiera, Stelvio M.

doi:10.1002/bmc.4135

Cited by 44 publications

(25 citation statements)

References 143 publications

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“…Clinical translation of these in vitro results to in vivo studies is not straightforward due to the low concentration of in vivo serotonin. Serotonin concentrations in human biological fluids have been measured as high as 283 ng/mL in whole blood 46 and in rat brain tissue as high as 29.5 ng/mg in the dorsal raphe nucleus, 47 which is approximately equivalent to 1.6 µM and 0.17 mM, respectively. However, the techniques used to measure serotonin concentration are uncertain, because they rely on extensive ex vivo tissue preparations that may significantly alter biochemical composition and do not accurately represent in vivo concentrations.…”

Section: Discussionmentioning

confidence: 99%

In vitro characterization of the serotonin biosynthesis pathway by CEST MRI

Oglesby

Lam

Stanisz

2020

Magnetic Resonance in Med

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Purpose The diagnosis of monoamine‐related psychiatric disorders is based on the phenomenological evaluation of symptoms and behavior by trained clinicians. The CEST technique can be sensitive to monoamines such as serotonin. This study quantifies the CEST properties of the compounds in the serotonin biosynthesis pathway with the goal of developing noninvasive techniques aimed at advancing the diagnostic assessment of serotonin dysfunction. Methods Saturation transfer‐weighted images of L‐tryptophan, 5‐hydroxytryptophan, serotonin, 5‐hydroxyindoleacetic acid, and melatonin phantoms were acquired over a range of saturation amplitudes and frequency offsets along with observed T1, T2, and B1 efficiency maps at physiological temperature and pH of 5.5, 6.7, and 7.4. The CEST and MT data were fitted to a three‐pool Bloch–McConnell model of exchange to estimate the model parameters. Results At a pH of 5.5, tryptophan, 5‐hydroxytryptophan and serotonin exhibited significant CEST contrast at resonance frequency offset, Δω between 2.64 ppm and 2.71 ppm, and magnetization transfer ratio asymmetry amplitudes up to 20% per 30 mM. At a pH of 7.4, all molecules exhibited significant CEST contrast between 5.11 ppm and 5.47 ppm, and magnetization transfer ratio asymmetry amplitudes up to 9.5% per 30 mM. At a pH of 6.7, all studied compounds except melatonin exhibited a CEST peak from each of the preceding two pHs. Conclusion At a pH of 5.5, tryptophan, 5‐hydroxytryptophan, and serotonin CEST contrast originates from the NH3+ side chain, whereas at a pH of 7.4, CEST contrast is due to the chemical exchange between water and the NH proton on the indole ring. The data in this study could be used to inform future investigations aimed at detecting and measuring in vivo serotonin.

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Section: Discussionmentioning

confidence: 99%

In vitro characterization of the serotonin biosynthesis pathway by CEST MRI

Oglesby

Lam

Stanisz

2020

Magnetic Resonance in Med

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“…Our findings suggest that 5-HT plays a significant role in determining the characteristics of CSC. For example, reduced 5-HT concentrations have been observed in patients with depression and panic attacks [26]. With respect to CSC, Sakai et al reported a mean blood 5-HT concentration of 98.2 ± 27.5 ng/mL in 34 patients with chronic CSC, which was significantly lower than those in 31 normal controls (128.5 ± 35.8 ng/mL) and 15 patients with acute CSC (122.5 ± 23.9 ng/mL) [19].…”

Section: Discussionmentioning

confidence: 99%

Central Serous Chorioretinopathy and Blood Serotonin Concentrations

Kondo

Araki

Komuku

et al. 2021

JCM

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Background: To investigate blood serotonin (5-hydroxytryptamine (5-HT)) concentrations and their relationships with selected characteristics in patients with central serous chorioretinopathy (CSC). Methods: This was a prospective study including 93 patients with active CSC. Blood concentrations of 5-HT, adrenocorticotropic hormone, and cortisol were measured in patients with CSC. Selected patient characteristics, including disease history (acute or chronic), medication use, smoking history, mood status, best-corrected visual acuity (BCVA), subfoveal choroidal thickness (SCT), findings on fluorescein and indocyanine green angiography, and anatomical changes were evaluated during follow-up. Results: Eleven of the 93 patients had low 5-HT concentrations (<57 ng/mL) (12%, eight men and three women; mean age 55 years); we identified no significant relationship with acute/chronic disease status. The patients with low 5-HT were significantly more likely to have five or more fluorescein leakage sites (p = 0.0275), recurrence of subretinal fluids (p < 0.0001), and failure to achieve significant improvement in BCVA during follow-up (p = 0.862) than patients with 5-HT within the normal range. Conclusions: Blood serotonin concentrations may influence the pathophysiology and prognosis of CSC.

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“…The open field test showed discrete differences between motor locomotion and social behavior expressed through anxiety/fear when comparing male and female control groups and groups treated with diacetyl. Females are less vulnerable to stress than males [ 55 ], but they are more affected by repeated stress than males [ 56 ]. Such behavioral changes may be related to changes in metabolic profile.…”

Section: Discussionmentioning

confidence: 99%

Effects of Diacetyl Flavoring Exposure in Mice Metabolism

Jedlicka

Silva

Balbino

et al. 2018

BioMed Research International

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Diacetyl is a flavoring that imparts a buttery flavor to foods, but the use or exposure to diacetyl has been related to some diseases. We investigated the effect of oral intake of diacetyl in male and female C57/Bl mice. We performed a target metabolomics assay using ultraperformance liquid chromatography paired with triple quadrupole mass spectrometry (UPLC-MS/MS) for the determination and quantification of plasmatic metabolites. We observed alterations in metabolites present in the urea and tricarboxylic acid (TCA) cycles. Peroxynitrite plasmatic levels were evaluated by a colorimetric method, final activity of superoxide dismutase (SOD) was evaluated by an enzymatic method, and mouse behavior was evaluated. Majority of the assay showed differences between control and treatment groups, as well as between genders. This may indicate the involvement of sex hormones in the regulation of a normal metabolic profile, and the implication of sex differences in metabolite disease response.

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Analysis and measurement of serotonin

Cited by 44 publications

References 143 publications

In vitro characterization of the serotonin biosynthesis pathway by CEST MRI

In vitro characterization of the serotonin biosynthesis pathway by CEST MRI

Central Serous Chorioretinopathy and Blood Serotonin Concentrations

Effects of Diacetyl Flavoring Exposure in Mice Metabolism

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