Analysis and interpretation of mixed forensic stains using DNA STR profiling

Clayton, Tim; Whitaker, Jonathan; Sparkes, Rebecca; Gill, Peter

doi:10.1016/s0379-0738(97)00175-8

Cited by 235 publications

(141 citation statements)

References 19 publications

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“…The novel Y-STRs have more than doubled the number of known pentanucleotide markers and they include the first hexanucleotide repeat on the NRY (DYS448). These longer repeat motif STRs may be useful for improving the interpretation of sample mixtures [31,32]. Depending on the particular STR, the stutter peak heights of dinucleotide and trinucleotide repeats can be higher than 30% of their corresponding STR allele, while stutter products of tetranucleotides are approximately 15%, and pentanucleotide repeats may have stutter products of less than 1-2% [19].…”

Section: Discussionmentioning

confidence: 99%

Forensic value of 14 novel STRs on the human Y chromosome

Redd

Agellon

Kearney

et al. 2002

Forensic Science International

146

106

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We identified and characterized 14 novel short-tandem-repeats (STRs) on the Y chromosome and typed them in two samples, a globally diverse panel of 73 cell lines, and 148 individuals from a European-American population. These Y-STRs include eight tetranucleotide repeats (DYS449, DYS453, DYS454, DYS455, DYS456, DYS458, DYS459, and DYS464), five pentanucleotide repeats (DYS446, DYS447, DYS450, DYS452, and DYS463), and one hexanucleotide repeat (DYS448). Sequence data were obtained to designate a repeat number nomenclature. The gene diversities of an additional 22 Y-STRs, including the most commonly used in forensic databases, were directly compared in the cell line DNAs. Six of the 10 most polymorphic markers include the newly identified Y-STRs. Furthermore, these novel Y-STRs greatly improved the resolution of paternal lineages, above the level obtained with commonly used Y-STRs, in the European-American population. #

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Section: Discussionmentioning

confidence: 99%

Forensic value of 14 novel STRs on the human Y chromosome

Redd

Agellon

Kearney

et al. 2002

Forensic Science International

146

106

View full text Add to dashboard Cite

show abstract

“…These extra bands are caused by enzyme slippage during PCR. 37,38 In general, the artefactual bands can be easily identified, since they exhibit a weaker product signal. With highly degraded aDNA, however, the amount of stutter products may exceed the quantity of the correct product 39,34 impeding determination of the samples' allelic composition.…”

Section: Chromosomal Dna Analysismentioning

confidence: 99%

Reconstruction of a historical genealogy by means of STR analysis and Y-haplotyping of ancient DNA

Gerstenberger¹,

Hummel²,

Schultes³

et al. 1999

Eur J Hum Genet

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“…This characteristic is an additional advantage because it can result in more than one human DNA profile (Clayton et al, 1998;Gill et al, 1998;González-Andrade et al, 2006) by female mosquitoes that may have fed on the victim or criminal. Therefore, the links between the suspect, victim, crime, and/or crime scene can be achieved by analyzing mosquitoes (Curic et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Trace samples of human blood in mosquitoes as a forensic investigation tool

et al. 2015

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Investigations of any type of crime invariably starts at the crime scene by collecting evidence. Thus, the purpose of this research was to collect and analyze an entomological trace from an environment that is similar to those of indoor crime scenes. Hematophagous mosquitoes were collected from two residential units; saliva of volunteers that were residents in the units was also collected for genetic analysis as reference samples. We examined the allele frequencies of 15 short tandem repeat loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, and FGA) and amelogenin. A total of 26 female hematophagous mosquitoes were identified as Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus; we were able to obtain 11 forensically valid genetic profiles, with a minimum of 0.028203 ng/μL of human DNA. Thus, the results of this study showed that it was possible to correlate human genetic information from mosquitoes with the volunteer reference samples, which validates the use of this information as forensic evidence. Furthermore, we observed mixed genetic profiles from one mosquito. Therefore, it is clearly important to collect these insects indoors where crimes were committed, because it may be possible to find intact genetic profiles of suspects in the blood found in the digestive tract of hematophagous mosquitoes for later comparison to identify an offender and/or exclude suspects

show abstract

Analysis and interpretation of mixed forensic stains using DNA STR profiling

Cited by 235 publications

References 19 publications

Forensic value of 14 novel STRs on the human Y chromosome

Forensic value of 14 novel STRs on the human Y chromosome

Reconstruction of a historical genealogy by means of STR analysis and Y-haplotyping of ancient DNA

Trace samples of human blood in mosquitoes as a forensic investigation tool

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