Analyses of transcriptome profiles and selected metabolites unravel the metabolic response to NH4+ and NO3− as signaling molecules in tea plant (Camellia sinensis L.)

Liu, Meiya; Burgos, Asdrúbal; Zhang, Qunfeng; Tang, Dandan; Shi, Yuanzhi; Ma, Lei; Yi, Xiaoyun; Ruan, Jianyun

doi:10.1016/j.scienta.2017.02.036

Cited by 36 publications

(20 citation statements)

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“…Thus, the tissue-specific response of gene expression could not be elucidated 30 . Recently, Liu et al reported that short-term (30 min) 10 mM NH 4 + -N or NO 3 − -N treatment significantly changed the expression of genes in multiple secondary metabolism pathways, and they proposed that NH 4 + and NO 3 − act as signaling agents in regulating gene expression 34 .…”

Section: Discussionmentioning

confidence: 99%

“…Basically, application of N fertilizers increases amino acid biosynthesis in tea plant. When equimolar concentrations of NO 3 − and NH 4 + were supplied, NH 4 + more efficiently promoted tea plant growth and amino acid accumulation 26,27,33,34 . In addition, intensive studies showed that shading treatment significantly increases free amino acid accumulation in tea plant 29,35,36 .…”

mentioning

confidence: 99%

“…In addition, intensive studies showed that shading treatment significantly increases free amino acid accumulation in tea plant 29,35,36 . The alteration of amino acid metabolism under various conditions was suggested to be associated with gene expression and activity of glutamine synthetase (GS), glutamate synthase (GOGAT), glutamate dehydrogenase (GDH) and other amino acid biosynthesis genes 26,34,36 . However, only a few genes involved in amino acid metabolism and genes associated with changes in amino acid accumulation have been identified in tea plants.…”

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confidence: 99%

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Transcriptional regulation of amino acid metabolism in response to nitrogen deficiency and nitrogen forms in tea plant root (Camellia sinensis L.)

Yang

Tai

et al. 2020

Sci Rep

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Free amino acids, including theanine, glutamine and glutamate, contribute greatly to the pleasant taste and multiple health benefits of tea. Amino acids in tea plants are mainly synthesized in roots and transported to new shoots, which are significantly affected by nitrogen (N) level and forms. However, the regulatory amino acid metabolism genes have not been systemically identified in tea plants. Here, we investigated the dynamic changes of free amino acid contents in response to N deficiency and forms in tea plant roots, and systemically identified the genes associated amino acid contents in individual metabolism pathways. Our results showed that glutamate-derived amino acids are the most dynamic in response to various forms of N and N deficiency. We then performed transcriptomic analyses of roots treated with N deficiency and various forms of N, and differentially expressed amino acid metabolic genes in each pathway were identified. The analyses on expression patterns and transcriptional responses of metabolic genes to N treatments provided novel insights for the molecular basis of high accumulation of theanine in tea plant root. These analyses also identified potential regulatory genes in dynamic amino acid metabolism in tea plant root. Furthermore, our findings indicated that the dynamic expression levels of CsGDH, CsAlaDC, CsAspAT, CsSDH, CsPAL, CsSHMT were highly correlated with changes of amino acid contents in their corresponding pathways. Herein, this study provides comprehensive insights into transcriptional regulation of amino acid metabolism in response to nitrogen deficiency and nitrogen forms in tea plant root.(Ser), glycine (Gly) and cysteine (Cys) synthesis. In tea plant, Thea is synthesized from Glu and ethylamine (EA) by theanine synthetase (TS) 14 . EA is likely produced from alanine under the catalysis of alanine decarboxylase 15 .Amino acid catabolism have been clearly described in animals, however, limited information of amino acid catabolism is available in plants. Hildebrandt et al. summarized the catabolic pathways of amino acids in land plants. Generally, amino acids are catabolized by oxidative deamination, oxidative decarboxylation, and transamination. These reactions are catalyzed by amino acid dehydrolases, decarboxylases and aminotransferases, respectively. Gln, Asn and Arg are also hydrolyzed by asparaginase, glutaminase, and arginase to release amide groups 16 .The accumulation of free amino acids is resultant of biosynthesis and catabolism. Previous studies showed that feedback inhibition loops control amino acid biosynthesis in plants 17 . Here, the accumulation of an amino acid inhibits the transcription or the activities of the enzymes in its biosynthesis pathway [18][19][20] . Expression of feedback-insensitive form of enzymes resulted in higher levels of the corresponding amino acids 17,21 . The central role of amino acid catabolism is to adjust the amino acid pool size, especially under stress conditions 12 . Some regulatory enzymes in amino acid metabolism have been ident...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Transcriptional regulation of amino acid metabolism in response to nitrogen deficiency and nitrogen forms in tea plant root (Camellia sinensis L.)

Yang

Tai

et al. 2020

Sci Rep

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“…Compared with the NH 4 + supply, the contents of chlorophyll and biomass were more lower in tea plants when NO 3 − was supplied as the sole nitrogen source [3]. Compared with NO 3 − supply, the biosynthesis of free amino acids and catechins was more effective in tea plants under NH 4 + supply, resulting from expression of N transporter genes [4]. NH 4 + deficiency could lead to clearly reduce the accumulation of amino acid, while NH 4 + supply could improve the status of carbohydrate in tea plants [1, 4].…”

Section: Introductionmentioning

confidence: 99%

Ammonium triggered the response mechanism of lysine crotonylome in tea plants

et al. 2019

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Background Lysine crotonylation, as a novel evolutionarily conserved type of post-translational modifications, is ubiquitous and essential in cell biology. However, its functions in tea plants are largely unknown, and the full functions of lysine crotonylated proteins of tea plants in nitrogen absorption and assimilation remains unclear. Our study attempts to describe the global profiling of nonhistone lysine crotonylation in tea leaves and to explore how ammonium (NH 4 + ) triggers the response mechanism of lysine crotonylome in tea plants. Results Here, we performed the global analysis of crotonylome in tea leaves under NH 4 + deficiency/resupply using high-resolution LC-MS/MS coupled with highly sensitive immune-antibody. A total of 2288 lysine crotonylation sites on 971 proteins were identified, of which contained in 15 types of crotonylated motifs. Most of crotonylated proteins were located in chloroplast (37%) and cytoplasm (33%). Compared with NH 4 + deficiency, 120 and 151 crotonylated proteins were significantly changed at 3 h and 3 days of NH 4 + resupply, respectively. Bioinformatics analysis showed that differentially expressed crotonylated proteins participated in diverse biological processes such as photosynthesis (PsbO, PsbP, PsbQ, Pbs27, PsaN, PsaF, FNR and ATPase), carbon fixation (rbcs, rbcl, TK, ALDO, PGK and PRK) and amino acid metabolism (SGAT, GGAT2, SHMT4 and GDC), suggesting that lysine crotonylation played important roles in these processes. Moreover, the protein-protein interaction analysis revealed that the interactions of identified crotonylated proteins diversely involved in photosynthesis, carbon fixation and amino acid metabolism. Interestingly, a large number of enzymes were crotonylated, such as Rubisco, TK, SGAT and GGAT, and their activities and crotonylation levels changed significantly by sensing ammonium, indicating a potential function of crotonylation in the regulation of enzyme activities. Conclusions The results indicated that the crotonylated proteins had a profound influence on metabolic process of tea leaves in response to NH 4 + deficiency/resupply, which mainly involved in diverse aspects of primary metabolic processes by sensing NH 4 + , especially in photosynthesis, carbon fixation and amino acid metabolism. The data might serve as important resources for exploring the roles of lysine crotonylation in N metabolism of tea plants. Data were available via ProteomeXchange with identifier PXD011610. Electronic supplementary material The online version of this article (10.1186/s12864-019-5716-z) contains supplementary material, which is available to authorized users.

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“…Tumbuhan menggunakan N untuk membentuk asam amino, yang selanjutnya dimasukkan ke dalam protein dan asam nukleat (DNA dan RNA). Nitrogen juga merupakan komponen integral klorofil, pigmen penyerap cahaya yang diperlukan untuk fotosintesis (McGrath et al 2014) N diserap dan dimanfaatkan oleh akar tanaman dalam bentukan organik Ammonium (NH4 + ) dan nitrat (NO3 -) (Liu et al 2017;Tang et al 2015). Input N yang berlebihan dalam ekosistem pertanian intensif telah menghasilkan penurunan efisiensi penggunaan N (EPN), serta peningkatan kehilangan N terhadap lingkungan, mencemari sistem air dan air melalui penguapan amonia (NH), penguapan permukaan, pencucian, dan denitrifikasi (Zhao, et al 2015;Li et al 2018;Uusheimo et al 2018).…”

Section: Pendahuluanunclassified

Teknik Isotop 15N untuk Mengevaluasi Pengaruh Biochar dan Bakteri Penambat Nitrogen terhadap Serapan Nitrogen Tanaman Padi Sawah

Bachtiar¹,

Nurrobifahmi²,

Citraresmini³

et al. 2020

J. Tanah dan Iklim

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Penelitian ini dilakukan untuk menguji pengaruh dari biochar yang berasal dari cangkang kelapa sawit dan juga keefektifan bakteri penambat N pada tanaman padi sawah varietas MIRA 1, dengan menggunakan teknik isotop 15 N. Rancangan penelitian yang digunakan yaitu Rancangan Acak Lengkap dengan 3 ulangan. Perlakuan yang diberikan meliputi K0 = Kontrol; K1 = NPK; B1 = Biochar 2 t ha-1 ; B2 = Biochar 4 t ha-1 ; B3 = Biochar 2 t ha-1 + NPK; B4 = Biochar 4 t ha-1 + ½ NPK; B5 = Biochar 2 t ha-1 + pupuk hayati ABC; B6 = Biochar 4 t ha-1 + pupuk hayati ABC; B7 = Biochar 2 t ha-1 + pupuk hayati ABC + ½ NPK; B8 = Biochar 4 t ha-1 + pupuk hayati ABC + ½ NPK; B9 = pupuk hayati ABC. Hasil penelitian menunjukkan bahwa pemberian perlakuan B8 (biochar 4 t ha-1 disertai dengan pupuk hayati ABC dan NPK ½ rekomendasi) mampu memberikan nilai serapan N dan hasil tanaman tertinggi. Teknik 15 N menunjukan bahwa sumbangan N tertinggi diperoleh dari perlakuan pupuk K1 (pemupukan NPK 100%). Biochar pada sawah memiliki efek pada peningkatan berat kering biji dan jerami, dan jika disertai dengan pupuk hayati, formula ABC + biochar dapat mengurangi penggunaan pupuk NPK hingga 50%.

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Analyses of transcriptome profiles and selected metabolites unravel the metabolic response to NH4+ and NO3− as signaling molecules in tea plant (Camellia sinensis L.)

Cited by 36 publications

References 42 publications

Transcriptional regulation of amino acid metabolism in response to nitrogen deficiency and nitrogen forms in tea plant root (Camellia sinensis L.)

Transcriptional regulation of amino acid metabolism in response to nitrogen deficiency and nitrogen forms in tea plant root (Camellia sinensis L.)

Ammonium triggered the response mechanism of lysine crotonylome in tea plants

Teknik Isotop 15N untuk Mengevaluasi Pengaruh Biochar dan Bakteri Penambat Nitrogen terhadap Serapan Nitrogen Tanaman Padi Sawah

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