Analyses of the results of different test systems in the 2005 global proficiency testing schemes for infectious bursal disease virus and Newcastle disease virus antibody detection in chicken serum

Abstract

The results of global proficiency testing schemes (PTS) for serological tests to detect antibodies against infectious bursal disease virus (IBDV) and Newcastle disease virus (NDV) in chicken serum, in which 125 and 120 laboratories, respectively, participated from Africa, Asia, Europe, Central and South America, were used to analyse the performances of different antibody test systems such as virus neutralization tests, haemagglutination inhibition tests, enzyme-linked immunosorbent assays and agar gel precipitation tests. All laboratories were asked to carry out their routine diagnostic tests for the detection of IBDV and NDV antibodies as usual. This global ring trial provided a large amount of data on variation within and between laboratories and test systems used worldwide. The data showed that the variation between the quantitative test results of different laboratories (R between ) using the IBDV virus neutralization tests and the NDV haemagglutination inhibition test was higher (about double) compared with the variation within commercial enzyme-linked immunosorbent assay systems. Although both tests are often referred to and used as the ''gold standard'' in experimental and scientific studies, official procedures and for the validation of tests, this study shows that there is an urgent need for a global implementation of recommended test procedures and/or the inclusion of international reference sera in these studies.

Serological response and protection level evaluation in chickens exposed to grains coated with I2 Newcastle disease virus for effective oral vaccination of village chickens

“…The level of ND virus antibodies in serum samples were determined using the HI test as described by OIE [ 5 ]. The HI test has 98 % specificity and 69–98 % sensitivity [ 19 ]. HI titration was made to determine the right HI concentration via 2-fold serial dilution of 25 μl sera in 25 μl PBS followed by 25 μl loading of viral antigen per well.…”

Evaluation of enzyme-linked immunosorbent assays and a haemagglutination inhibition tests for the detection of antibodies to Newcastle disease virus in village chickens using a Bayesian approach

“…Since HI is commonly used in NDV antibody diagnosis its performance has rarely been questioned. However, studies have shown that the variation between the quantitative test results of different laboratories using the NDV HI test was higher (about double) compared with the variation within commercial ELISA systems (de Wit et al, 2007).…”

“…Therefore, serological tests such as ELISA and HI are often relied upon for the laboratory diagnosis of ND or surveys to estimate serological prevalence, indirectly through the detection of antibodies (target condition) produced following infection by ND virus (NDV). However, these tests have limitations due to uncertainty regarding their diagnostic accuracy, as well as, lack of reproducibility under different laboratory conditions (Beard and Wilkes, 1985;Schelling et al, 1999;de Wit et al, 2007). For instance, NDV has been reported to show some degree of cross-reactivity in HI tests with several of the other avian paramyxovirus serotypes, especially APMV-3 psittacine isolates, using polyclonal antisera (Alexander et al, 1983;Lipkind and Shihmanter, 1986;Adair et al, 1989).…”

Evaluation of Serum Conversion Induced by Infectious Bursal Disease Vaccines Submitted to Official Quality Control in the Period 2013 to April 2014

“…The result of antibody titer obtained in monovalent vaccines Winterfield strain for us is concordant of the find by Nishizawa M, et al [9] the vaccine strain induced high IBD antibody titer. De Witt JJ, et al [10] showing the results of global proficiency testing schemes (PTS) for serological tests to detect antibodies against IBDV in chicken serum, in which 125 laboratories participated from Africa, Asia, Europe, Central and South America using: virus neutralization (VN) enzyme-linked immunosorbent assays (ELISA) and agar gel precipitation (AGP). All laboratories were asked to carry out their routine diagnostic tests for the detection of IBDV antibodies as usual.…”