Analyses of regenerative bone matrix of rat tibia after laser photo-excitation by SEM and AFM

Baranauskas, Vı́tor; Garavello, Ivânia; Jingguo, Zhao; Cruz‐Höfling, Maria Alice da

doi:10.1016/j.apsusc.2005.03.097

Cited by 6 publications

(7 citation statements)

References 12 publications

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“…To the best of our knowledge, this work is the first to use AFM and micro‐CT in combination to compare the progression of the regeneration induced by BMP‐2 formulated in different scaffolds. Previous work has included examining tibiae repair of rats after laser excitation by AFM and SEM and a characterizations of the bone formed by the implantation of MSCs overexpressing the BMP‐2 gene in ectopic and orthotopic defects in mice, with analysis occurring at fixed time points.…”

Section: Discussionmentioning

confidence: 99%

“…Although the values of the nanomechanical properties measured by AFM are stronger affected by the hydration conditions of the samples poor attention has been paid to this aspect. AFM has been used to characterize the regenerative effect of osteo‐inductive stimulus in bone defects in a limited number of cases . By contrast, micro X‐ray computed tomography (micro‐CT) is frequently used to evaluate the bone regeneration induced by external factors such as BMP‐2 included in different scaffolds applied to different defects …”

Section: Introductionmentioning

confidence: 99%

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Evaluation of nanostructure and microstructure of bone regenerated by BMP‐2‐porous scaffolds

Rosario

Rodríguez-Évora

Reyes

et al. 2015

J Biomedical Materials Res

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In this study, three systems containing BMP-2 were fabricated, including two electrospun sandwich-like-systems of PLGA 75:25 and PLGA 50:50 and a microsphere system of PLGA 50:50 to be implanted in a critical size defect in rat calvaria. The in vivo BMP-2 release profiles of the three systems were similar. The total dose was released during the first two weeks. To evaluate the nano and microstructure of the regenerated bone a multi-technique analysis was used, including stereo microscope, X-Ray; AFM, micro-CT, and histological analyses. The progression of bone regeneration was followed at 4, 8, and 12 weeks after the microsphere system implantation whereas the two electrospun systems were evaluated at fixed 12 weeks. All the techniques applied showed high bone regeneration. The average values of bone volume density, bone mineral density, Young's modulus, and the percent of bone repair were ∼70% of the values of the native bone. Besides, SEM-EDX analysis indicated that the main chemical elements in the new bone were oxygen, calcium, and phosphorus in a ratio similar to that of native bone. In comparison, the micro-CT may provide an alternative to histology for the evaluation of bone formation at the defect size.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of nanostructure and microstructure of bone regenerated by BMP‐2‐porous scaffolds

Rosario

Rodríguez-Évora

Reyes

et al. 2015

J Biomedical Materials Res

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show abstract

“…These distal ends were dipped in 2.5% glutaraldehyde for 24 h, and immersed in 10% sodium hypochlorous for 6 h, then washed in ultrasonic cleaner (KQ5200DE, Kunshan Ultrasonic Instrument, Kunshan, China) for 20 min [17,18]. Bones were then cut (using a Dremil tool with a small drill bit) to produce flat, smooth cross-sectional samples.…”

Section: Scanning Electron Microscope Observation and Bone Histomorphmentioning

confidence: 99%

Protective effects of bovine colostrum acid proteins on bone loss of ovariectomized rats and the ingredients identification

et al. 2011

Molecular Nutrition Food Res

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“…Baranauskas et al used AFM to assess the spatial organization of newly formed bone following a surgically-induced injury [44] and [45]. The most commonly measured property of the collagen network in bone is fibril diameter.…”

Section: Investigating Collagen In Bonementioning

confidence: 99%

Applications of atomic force microscopy for the assessment of nanoscale morphological and mechanical properties of bone

Wallace¹

2012

Bone

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Scanning probe microscopy (SPM) has been in use for 30 years, and the form of SPM known as atomic force microscopy (AFM) has been around for 25 of those years. AFM has been used to produce high resolution images of a variety of samples ranging from DNA to carbon nanotubes.Type I collagen and many collagen-based tissues (including dentin, tendon, cartilage, skin, fascia, vocal cords, and cornea) have been studied with AFM, but comparatively few studies of bone have been undertaken. The purpose of this review is to introduce the general principles of AFM operation, demonstrate what AFM has been used for in bone research, and discuss the new directions that this technique can take the study of bone at the nanoscale.

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Analyses of regenerative bone matrix of rat tibia after laser photo-excitation by SEM and AFM

Cited by 6 publications

References 12 publications

Evaluation of nanostructure and microstructure of bone regenerated by BMP‐2‐porous scaffolds

Evaluation of nanostructure and microstructure of bone regenerated by BMP‐2‐porous scaffolds

Protective effects of bovine colostrum acid proteins on bone loss of ovariectomized rats and the ingredients identification

Applications of atomic force microscopy for the assessment of nanoscale morphological and mechanical properties of bone

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