Evaluation of nanostructure and microstructure of bone regenerated by BMP‐2‐porous scaffolds

Rosario, Carlos del; Rodríguez-Évora, María; Reyes, Ricardo; Orive, Alejandro González; Creus, Alberto Hernández; Shakesheff, Kevin M.; White, Lisa J.; Delgado, Araceli; Évora, Carmen

doi:10.1002/jbm.a.35436

Cited by 11 publications

(5 citation statements)

References 35 publications

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“…There are several types of bone growth factors, including bone morphogenetic growth factors (BMP), fibroblast growth factor (FGF), platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), and insulin-like growth factor (IGF). Among all these growth factors, BMP is considered to be the most effective way to format bone tissue during embryonic development, growth, and healing and throughout adulthood [18,19]. An increasing number of reports tested the in vivo application and biological foundation when BMP is used in bone defects [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Dual-Drug-Loaded Silk Fibroin/PLGA Scaffolds for Potential Bone Regeneration Applications

Yao

Wang

et al. 2019

Journal of Nanomaterials

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Developing scaffold materials with excellent biocompatibility, mechanical properties, and controlled drug release properties is vital to tissue engineering. In this study, we fabricated silk fibroin (SF)/poly(lactide-co-glycolide) (PLGA) nanofiber scaffolds containing recombinant human bone morphogenetic protein 2 (rhBMP2) and dexamethasone (DXM) via coaxial electrospinning, which were used in in vitro bone formation with rat bone marrow mesenchymal stem cells (rBMSCs). An in vitro drug release study was adopted to evaluate the sustained release potential of the core-shell structured nanofibers. Furthermore, we detected the potential of the SF/PLGA nanofiber membrane in vitro. In vitro studies showed that rhBMP2 still remained active on the nanofiber membrane. In addition, the dual-drug-loaded nanofiber membrane showed an early burst release of DXM and late sustained release of rhBMP2. rhBMP2 and DXM exhibited strong osteogenic differentiation potential when they acted on rBMSCs. Therefore, the SF/PLGA nanofiber membrane loaded with rhBMP2 and DXM has great potential for the enhancement of bone regeneration.

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Section: Introductionmentioning

confidence: 99%

Dual-Drug-Loaded Silk Fibroin/PLGA Scaffolds for Potential Bone Regeneration Applications

Yao

Wang

et al. 2019

Journal of Nanomaterials

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“…Since the past decade, PLGA has been shown to be a potential scaffold for bone tissue engineering, ,, cartilage tissue engineering, , ligament/tendon tissue engineering, and liver tissue engineering . Furthermore, the kinetics of release profile from PLGA scaffolds including SDF-1 has been investigated extensively − in order to aid their clinical translation. However, their use in clinics as biodegradable devices still warrants more understanding regarding their mechanical properties and degradation behavior.…”

Section: Discussionmentioning

confidence: 99%

Chemokine SDF1 Mediated Bone Regeneration Using Biodegradable Poly(D,L-lactide-co-glycolide) 3D Scaffolds and Bone Marrow-Derived Mesenchymal Stem Cells: Implication for the Development of an “Off-the-Shelf” Pharmacologically Active Construct

et al. 2020

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There is an increasing need for bone substitutes for reconstructive orthopedic surgery following removal of bone tumors. Despite the advances in bone regeneration, the use of autologous mesenchymal stem cells (MSC) presents a significant challenge, particularly for the treatment of large bone defects in cancer patients. This study aims at developing new chemokine-based technology to generate biodegradable scaffolds that bind pharmacologically active proteins for regeneration/repair of target injured tissues in patients. Primary MSC were cultured from the uninvolved bone marrow (BM) of cancer patients and further characterized for “stemness”. Their ability to differentiate into an osteogenic lineage was studied in 2D cultures as well as on 3D macroporous PLGA scaffolds incorporated with biomacromolecules bFGF and homing factor chemokine stromal-cell derived factor-1 (SDF1). MSC from the uninvolved BM of cancer patients exhibited properties similar to that reported for MSC from BM of healthy individuals. Macroporous PLGA discs were prepared and characterized for pore size, architecture, functional groups, thermostability, and cytocompatibility by ESEM, FTIR, DSC, and CCK-8 dye proliferation assay, respectively. It was observed that the MSC+PLGA+bFGF+SDF1 construct cultured for 14 days supported significant cell growth, osteo-lineage differentiation with increased osteocalcin expression, alkaline phosphatase secretion, calcium mineralization, bone volume, and soluble IL6 compared to unseeded PLGA and PLGA+MSC, as analyzed by confocal microscopy, biochemistry, ESEM, microCT imaging, flow cytometry, and EDS. Thus, chemotactic biomacromolecule SDF1-guided tissue repair/regeneration ability of MSC from cancer patients opens up the avenues for development of “off-the-shelf” pharmacologically active construct for optimal repair of the target injured tissue in postsurgery cancer patients, bone defects, damaged bladder tissue, and radiation-induced skin/mucosal lesions.

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“…The forcedisplacement of samples was measured in map scanning mode with an area of 300 nm × 300 nm under the PeakForce Tapping Model. Data were analyzed by nanoscope analysis software to get the compressive property of fibers according to the Sneddon model [27].…”

Section: Micro-compressive Propertymentioning

confidence: 99%

Improvement of mechanical properties of zein porous scaffold by quenching/electrospun fiber reinforcement

Liu¹,

Yang²,

Shen³

et al. 2021

Biomed. Mater.

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As a novel bone substitute material, zein-based scaffolds (ZS) should have suitable mechanical properties and porosity. ZS has shown good compressive properties matching cancellous bone, but there is still a demand to improve its mechanical properties, especially tensile and bending properties without adding plasticizers. The present study explored two simple and environment-friendly factors for this purpose: fiber reinforcement and quenching. Addition of electrospun zein fibers enhanced all mechanical properties significantly including compressive, tensile, and bending moduli; compressive and bending strengths of ZS with both higher (70–80%) and lower (50–60%) porosities, no matter whether heating treated or not treated. Especially, all these parameters were further enhanced significantly by addition of heating treated fibers. AFM provided evidence that high temperature modification could significantly alter the micro-elastic properties of zein electrospun fibers, i.e., increased stiffness of fibers. Quenching treatment also enhanced compressive, tensile, and bending strengths significantly. Finally, quenching treated ZS were implanted into critical-sized bone defects (15 mm) of the rabbit model to compare the repair efficacy with a commercial β-tricalcium phosphate product. The results demonstrated that there were no remarkable differences in bone reconstructions between these two materials.

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Evaluation of nanostructure and microstructure of bone regenerated by BMP‐2‐porous scaffolds

Cited by 11 publications

References 35 publications

Dual-Drug-Loaded Silk Fibroin/PLGA Scaffolds for Potential Bone Regeneration Applications

Dual-Drug-Loaded Silk Fibroin/PLGA Scaffolds for Potential Bone Regeneration Applications

Chemokine SDF1 Mediated Bone Regeneration Using Biodegradable Poly(D,L-lactide-co-glycolide) 3D Scaffolds and Bone Marrow-Derived Mesenchymal Stem Cells: Implication for the Development of an “Off-the-Shelf” Pharmacologically Active Construct

Improvement of mechanical properties of zein porous scaffold by quenching/electrospun fiber reinforcement

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