Analyses of G-bands in chromosomes of the Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae).

Tambasco, A. J.; Giannoni, Marcos A.; Moreira, Lília Maria de Azevedo

doi:10.1508/cytologia.44.21

Cited by 7 publications

(3 citation statements)

References 2 publications

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“…1C and Fig. 2B) was similar to the G banding pattern of the species M. quadrifasciata described by Tambasco et al (1979). A correspondence between the Hae III and G banding patterns has been detected in many animals (Miller et al .…”

Section: Resultssupporting

confidence: 84%

Longitudinal differentiation in Melipona mandacaia (Hymenoptera, Meliponini) chromosomes

et al. 2003

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Melipona mandacaia is a stingless bee endemic to northeast Brasil. We describe the M. mandacaia karyotype using C-banding technique. fluorochrome staining and treatment with restriction enzymes and discuss the position of this species in the context of the phylogeny of the genus. Melipona mandacaia has 2n = 18 (14 SM + 2 M + 2 A). Heterochromatin was detected in the pericentromeric region of pairs 1, 2 and 8 and in the form of small blocks in the remaining pairs. Staining with base-specific fluorochromes showed that this heterochromatin was rich AT (QM and DAPI), except in the region corresponding to the NOR which was rich GC (CMA3) and was cleaved by the HaeIII enzyme. Melipona mandacaia is a member of Group I Melipona. Treatment with DraI/Giemsa discloses a larger number of bands than treatment with DraI/QM. Pre-cleavage with DraI gave rise to a larger number of bands following QM staining; a circumstance evidently due to a removal of the DNA-protein complex that prevented the association of the fluorochrome with AT-rich DNA. The results highlight the complex nature of heterochromatin.

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Section: Resultssupporting

confidence: 84%

Longitudinal differentiation in Melipona mandacaia (Hymenoptera, Meliponini) chromosomes

et al. 2003

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“…The G-banding patterns on the chromo somes of M. quadricasciata anthidioides (Tambasco et al 1979) are different from those of honey bees (Hoshiba and Okada 1986). Thus, chromosome common between Melipona and Apis which suggests a common ancestor seems not to be found in the present study.…”

Section: Acceptedcontrasting

confidence: 66%

Karyological analysis of a stingless bee, Melipona favosa (Apidae, Hymenoptera).

Hoshiba

1988

CYTOLOGIA

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“…So far, sixteen species have been analyzed cytogenetically. Fifteen of them are 2n=18, and one is 2n=20 (K err 1948, 1952, 1972, K err and S ilveira 1972; T ambasco et al 1979; A lmeida 1981; H oshiba 1988; P ompolo 1992, 1994; H oshiba and I mai 1993; R ocha and P ompolo 1998).…”

Section: Introductionmentioning

confidence: 99%

DNA characterization and karyotypic evolution in the bee genus Melipona (Hymenoptera, Meliponini)

et al. 2002

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We analyzed patterns of heterochromatic bands in the Neotropical stingless bee genus Melipona (Hymenoptera, Meliponini). Group I species (Melipona bicolor bicolor, Melipona quadrifasciata, Melipona asil6ae, Melipona marginata, Melipona subnitida) were characterized by low heterochromatic content. Group II species (Melipona capixaba, Melipona compressipes, Melipona crinita, Melipona seminigra fuscopilosa e Melipona scutellaris) had high heterochromatic content. All species had 2n =18 and n = 9. In species of Group I heterochromatin was pericentromeric and located on the short arm of acrocentric chromosomes, while in Group II species heterochromatin was distributed along most of the chromosome length. The most effective sequential staining was quinacrine mustard (QM)/distamycin (DA)/chromomycin A 3 (CMA 3 )/4-6-diamidino-2-phenylindole (DAPI). Heterochromatic and euchromatic bands varied extensively within Group I. In Group II species euchromatin was restricted to the chromosome tips and it was uniformly GC + . Patterns of restriction enzymes (EcoRI, DraI, HindIII) showed that heterochromatin was heterogeneous. In all species the first pair of homologues was of unequal size and showed heteromorphism of a GC + pericentromeric heterochromatin. In M. asil6ae (Group I) this pair bore NOR and in M. compressipes (Group II) it hybridized with a rDNA FISH probe. As for Group I species the second pair was AT + in M. subnitida and neutral for AT and GC in the remaining species of this group. Outgroup comparison indicates that high levels of heterochromatin represent a derived condition within Melipona. The pattern of karyotypic evolution sets Melipona in an isolated position within the Meliponini.

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Analyses of G-bands in chromosomes of the Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae).

Cited by 7 publications

References 2 publications

Longitudinal differentiation in Melipona mandacaia (Hymenoptera, Meliponini) chromosomes

Longitudinal differentiation in Melipona mandacaia (Hymenoptera, Meliponini) chromosomes

Karyological analysis of a stingless bee, Melipona favosa (Apidae, Hymenoptera).

DNA characterization and karyotypic evolution in the bee genus Melipona (Hymenoptera, Meliponini)

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