Analisis Tindak Balas Berantai Polimerase (PCR) Simpleks dan Multipleks ke atas Produk Surimi Terawat Terma bagi Pengesanan DNA Lembu dan Babi

Shahimi, Safiyyah; Ghazali, Nur Syafiqa Akmal Ahmad; Elias, Aishah; Zaini, Nurul Aqilah Mohd; Mutalib, Sahilah Abd.

doi:10.17576/jsm-2020-4908-18

Cited by 2 publications

(1 citation statement)

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“…Hence, this study serves as a reference for stakeholders in the food industry, as well as Muslim consumers. PCR is an easy, fast and reproducible technique to identify species-specific animal DNA in the event of contamination by blood sources [ 29 ]. In this study, we compared DNA detection between bovine serum and bovine blood plasma.…”

Section: Introductionmentioning

confidence: 99%

Species-Specific Deoxyribonucleic Acid (DNA) Identification of Bovine in Cultured Meat Serum for halal Status

Kashim

Haris

Hasim

et al. 2022

Foods

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Meat culturing technology goes beyond laboratory research and materialises in the market. Nonetheless, this technology has raised concerns among Muslim consumers worldwide due to its medium, especially foetal bovine serum (FBS), which originates from blood. Thus, the aim of this research was to determine the halal status of cultured meat by detecting species-specific DNA of bovine serum as one of the media used during meat production. Polymerase chain reaction (PCR) analysis was conducted by targeting mitochondrial cytochrome oxidase II (COII) gene sequences, producing a 165 bp amplicon. The sequences of the primers used were Bovine-F, 5′-CAT CAT AGC AAT TGC CAT AGT CC-3′ and Bovine-R, 5′-GTA CTA GTA GTA TTA GAG CTA GAA TTA G-3′. DNA extraction was conducted using a QIAGEN Blood and Tissue™ commercial kit. The presence study also included a literature review on the Istihalah (transformation) concept in order to determine the halal status of cultured meat. The results revealed that bovine DNA was detected in all samples tested using PCR analysis. Therefore, Istihalah tammah (perfect transformation) does not occur due to the ability of PCR analysis to detect bovine DNA in FBS and is prohibited according to Shariah law.

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Section: Introductionmentioning

confidence: 99%