Temperature control algorithm for polymerase chain reaction (PCR) instrumentation based upon improved hybrid fuzzy proportional integral derivative (PID) control

Liu, Haoran; Fang, Yile; Su, Xiangyi; Wang, Yue; Ji, Minjie; Xing, Hongbing; Gao, Yue; Zhang, Yuanying; He, Nongyue

doi:10.1080/10739149.2022.2105866

Cited by 7 publications

(4 citation statements)

References 69 publications

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“…Cycle" in 1987 [150], the first automated thermal cycler that heated and cooled using a metal block, and the use of a thermostable DNA polymerase (Thermus aquaticus) in 1988 [5], are the two features that pushed PCR into a new era. As significant advancements in technology were made, the PCR process became faster, with mineral oil being replaced by heated lids (preventing sample evaporation and condensation) and bulky plumbing compressors traded for Peltier blocks with temperature control algorithms that can heat and cool rapidly [150,151]. The significant increase in machine ramp rates and heat dispersal rates within the PCR process in recent years has allowed the speed and throughput of PCR to increase substantially.…”

Section: Evolution Of Pcr Cycling Conditions In Other Disciplinesmentioning

confidence: 99%

PCR in Forensic Science: A Critical Review

McDonald,

Taylor,

Linacre

2024

Genes

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The polymerase chain reaction (PCR) has played a fundamental role in our understanding of the world, and has applications across a broad range of disciplines. The introduction of PCR into forensic science marked the beginning of a new era of DNA profiling. This era has pushed PCR to its limits and allowed genetic data to be generated from trace DNA. Trace samples contain very small amounts of degraded DNA associated with inhibitory compounds and ions. Despite significant development in the PCR process since it was first introduced, the challenges of profiling inhibited and degraded samples remain. This review examines the evolution of the PCR from its inception in the 1980s, through to its current application in forensic science. The driving factors behind PCR evolution for DNA profiling are discussed along with a critical comparison of cycling conditions used in commercial PCR kits. Newer PCR methods that are currently used in forensic practice and beyond are examined, and possible future directions of PCR for DNA profiling are evaluated.

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Section: Evolution Of Pcr Cycling Conditions In Other Disciplinesmentioning

confidence: 99%

PCR in Forensic Science: A Critical Review

McDonald,

Taylor,

Linacre

2024

Genes

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“…Haoran et al (Liu et al 2023) proposed the F-PID controller for the chemical industry's polymerase chain reaction (PCR). The relay self-tuning algorithm has been proposed for the initial generation of fuzzy parameters.…”

Section: Fuzzy-pid Controller For Chemical Industry Applicationmentioning

confidence: 99%

Review on PID, fuzzy and hybrid fuzzy PID controllers for controlling non-linear dynamic behaviour of chemical plants

Mohindru

2024

Artif Intell Rev

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The chemical production process is tedious due to the integration of different types of equipment and variables. Designing the controller is crucial in the chemical industry due to the interactive and non-linear system behaviour. An intelligent autonomous controller can improve the operating efficiency of the industry. Although several controllers have been developed, different system failures are frequently reported. Hence, controllers such as proportional integral derivative (PID), fuzzy logic controller (FLC), and hybrid fuzzy PID (F-PID) applied in the chemical industries are critically reviewed in the paper. Initially, the PID controller-based approaches are reviewed for different purposes in the chemical industry. After that, the FLC-based controllers-based papers are reviewed. In order to satisfy the issues in both controllers, the H-PID controllers have been reviewed. This review paper will provide an effective solution for operation control in the chemical industry under different operating conditions.

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“…Such defects can lead to differences in the optical path lengths between edge wells and central wells of qPCR instrument Block resulting in variations in the excitation light energy received by fluorescent groups in different wells, and ultimately affecting the reliability of experimental results, namely Ct values. Similarly, temperature differences between wells within the Block can also lead to such edge effects with even small optical or temperature differences between wells may be exponentially amplified during the amplification process, ultimately affecting experimental results [14,15].…”

Section: Of 22mentioning

confidence: 99%

Physical Simulation-Based Calibration for Quantitative Real-Time PCR

Zhu,

Liu,

Xiao

2024

Applied Sciences

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The fluorescence quantitative polymerase chain reaction (qPCR) instrument has been widely used in molecular biology applications, where the reliability of the qPCR performance directly affects the accuracy of its detection results. In this paper, an integrated, physics-based calibration device was developed to improve the accuracy and reliability of qPCR, realizing the calibration of qPCR instruments’ standard curve through physical simulations. With this calibration device, the collected temperature was used as the control signal to alter the fluorescence output, which allowed different probes to simulate the Ct values corresponding to samples with varying initial concentrations. The temperature and optical performance of this calibration device were tested, followed by a comparative analysis comparing the on-machine test results with standard substances to assess the linearity and uniformity of the Ct values of the measured qPCR instrument. It has been proven that this physical calibration device can effectively replace the biochemical standard substance to carry out comprehensive calibration of the temperature and optical parameters of the qPCR instrument and provide a more reliable method for the periodic calibration and quality control of the qPCR instrument. This contributes to the accuracy and reliability of fluorescence qPCR instruments in the field of molecular biology.

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Temperature control algorithm for polymerase chain reaction (PCR) instrumentation based upon improved hybrid fuzzy proportional integral derivative (PID) control

Cited by 7 publications

References 69 publications

PCR in Forensic Science: A Critical Review

PCR in Forensic Science: A Critical Review

Review on PID, fuzzy and hybrid fuzzy PID controllers for controlling non-linear dynamic behaviour of chemical plants

Physical Simulation-Based Calibration for Quantitative Real-Time PCR

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