Análise de fatores preditivos de ressecção visceral no tratamento operatório de doentes portadores de hérnia incisional gigante com perda de domicílio submetidos a pneumoperitônio progressivo pré-operatório

Tanaka, Eduardo

doi:10.11606/t.5.2009.tde-31082009-151511

Cited by 2 publications

(3 citation statements)

References 41 publications

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“…Kountouras et al 11 , using rats, evaluated the changes in spleen weight, relating tenths of a gram of organ weight per each hundred grams of body weight. The authors of this paper made the option for the use of a mathematical formula which calculates the volume of an elliptical form of a geometric figure, however tridimensional (ellipsoid) as recommended by Tanaka 21 , for the reason that the method can be carried out in the research laboratory. The spleen volume increased slightly with the growth of the animal in the control group (sham-operated), but showed a higher increase when were assessed the animals that underwent partial portal vein ligation, both after two and four weeks of study.…”

Section: Discussionmentioning

confidence: 99%

“…The spleens volume were calculated from specific mathematical formula: V=4/3 x πx(r1 x r2 x r3), wherein the variables V, r1, r2 , r3 represent the volume and the radius length, width and the thickness, since the body may be compared to a three-dimensional elliptical geometric figure (ellipsoid) 21 , shown in Figure 6. Groups 1, 2 and 3 were compared and the results found in operative procedures were divided into two subgroups, designated A, the result of the first operation, and B, results of reoperation.…”

Section: Modelos Animaismentioning

confidence: 99%

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Aumento do volume do baço em modelo experimental de hipertensão portal pré-hepática em ratos

Silva

Kriguer-Júnior

Serigiolle

et al. 2013

ABCD, arq. bras. cir. dig.

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Section: Discussionmentioning

confidence: 99%

Section: Modelos Animaismentioning

confidence: 99%

Aumento do volume do baço em modelo experimental de hipertensão portal pré-hepática em ratos

Silva

Kriguer-Júnior

Serigiolle

et al. 2013

ABCD, arq. bras. cir. dig.

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“…Then samples were examined and weighed individually, and the length, width, and thickness were measured using a Digital Vernier caliper (LOUISWARE, China). At the same time, the volume was calculated using the mathematical formula V = 4/3 * π * A * B * C where V , A , B , C represent the volume, length, width, and thickness since the body of spleen typically 3- dimensional elliptical Figure ( Tanaka, 2009 ). Then samples were immersed into 10% buffered formalin and kept for 48 hours.…”

Section: Methodsmentioning

confidence: 99%

Morphological, histological, and histochemical study of the adult golden hamster (Mesocricetus auratus) spleen

Hasan¹,

Altaey²,

Sultan³

2023

Open Vet J

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Background: The golden hamster is a choice model for investigating many visceral and splenic infections and neoplastic and retrospective lesions. Aim: To study hamsters' spleen's morphological, histological, and histochemical structure. Methods: Samples were collected from eight healthy adult golden hamsters and then fixed with 10% buffered formalin. Later, samples were processed, sectioned, and stained with Hematoxylin and Eosin as well as Masson’s Trichrome stain. Other slides were further stained with Periodic Acid Schiff and Alcian blue 2.5 stain (PAS) for histochemical evolution; the gross measurement was performed for the splenic length, width, and thickness, while the histological measures included the splenic capsular and trabecula thickness, diameter of white pulp follicles, splenic sinusoids and central arteries and proportion of white and red pulps. Results: The macroscopic findings revealed that the spleen was red-brown lanciform on the left side of the dorsolateral abdominal wall. The morphological measurements for splenic length, width, and thickness were 26.6 ± 7.67, 4.17 ± 1.65, and 1.70 ± 0.01 mm, respectively. The histological observations showed that the splenic capsule was composed of two layers (serosal and subserosal). The inner layer sends trabeculae dividing the splenic parenchyma irregularly, and the splenic parenchyma comprises the white and red pulp. The white pulp follicles included the mantle, marginal zones, and the PALS (periarterial lymphatic sheath), while the red pulp constituted splenic cords and sinuses. The histomorphological findings showed that white pulp follicles and the central artery mean diameter were 252.62 ± 8.07 µm and 54.45 ± 0.36 µm respectively, the proportion of white to a red pulp was 0.49 ± 0.01, the splenic capsule, trabecula and the wall of splenic arteries showed an intense positive activity to PAS stain and negative or weak in other splenic structures. Conclusion: The similarities and differences in the spleen between the laboratory animals and hamsters were apparent in this article, so understanding the morphological and histological structure of the spleen presents significant assistance with species identification to select the appropriate experimental animal model in future medical research

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Análise de fatores preditivos de ressecção visceral no tratamento operatório de doentes portadores de hérnia incisional gigante com perda de domicílio submetidos a pneumoperitônio progressivo pré-operatório

Cited by 2 publications

References 41 publications

Aumento do volume do baço em modelo experimental de hipertensão portal pré-hepática em ratos

Aumento do volume do baço em modelo experimental de hipertensão portal pré-hepática em ratos

Morphological, histological, and histochemical study of the adult golden hamster (Mesocricetus auratus) spleen

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