Analgesic Effects of Callus Culture Extracts from Selected Species of Phyllanthus in Mice

Abstract

The aim of this study was to evaluate the analgesic effect of the methanolic extract from callus culture of Phyllanthus tenellus, P. corcovadensis and P. niruri in several models of pain in mice. The extracts (medium containing 2,4-dichlorophenoxyacetic acid) of P. corcovadensis, P. niruri and P. tenellus (3-90 mg kg-1, i.p.) caused graded inhibition of abdominal constrictions induced by acetic acid (0.6%), with ID50 (i.e. dose that reduced response of control by 50%) values of about 30, 19 and > 30 mg kg-1, respectively. The extract of callus of Phyllanthus obtained in indole-3-butyric acid and indole-3-acetic acid media (3-90 mg kg-1, i.p.) caused a similar analgesic effect. In the formalin test, the extract of P. tenellus obtained in indole butyric acid medium (3-100 mg kg-1, i.p.) inhibited only the second phase of formalin-induced pain with an ID50 value of about 100 mg kg-1. Both the indole acetic acid and indole butyric acid methanolic extracts of P. tenellus and P. corcovadensis (10-100 mg kg-1, i.p.) dose-dependently inhibited both phases of formalin-induced pain (ID50 values for the second phase were approx. 100 and 52 mg kg-1, respectively). However, the extract of callus from Phyllanthus failed to affect formalin-induced paw oedema, as well as the response to radiant heat in the tail-flick test. In addition, the analgesic effect of morphine, but not the analgesic effects caused by Phyllanthus callus extract, was fully antagonized by naloxone.(ABSTRACT TRUNCATED AT 250 WORDS)

Inhibitory Effects of Methyl Brevifolincarboxylate Isolated from Phyllanthus niruri L. on Platelet Aggregation

“…P. niruri has been used for the treatment of urinary disturbances, intestinal infections, diabetes, and hepatitis. 4) Previous studies have revealed bioactivities such as antiplasmodial and antimalarial, 5,6) urolithiasis, 7) antiinflammatory, 8) and antihepatitis effects. 9) Chemical constituents such as tannins, 9,10) lignans, 11) polysaccharides, 12) alkaloid, 13) and flavonoids 14) were previously isolated.…”

In vitro culture of Phyllanthus stipulatus (Euphorbiaceae)

“…are on callus cultures of P. emblica, P. urinaria, P. amarus, P. abnormis, P. caroliniensis, P. tenellus, and P. niruri and on transformed root cultures of P. niruri (Khanna & Nag 1973, Unander 1991, Ishimaru et al 1992, Santos et al 1994. Pharmacological studies carried out with callus extracts of P. niruri, P. tenellus and P. urinaria have shown antinociceptive properties and the main compounds identified in the extracts were flavonoids, tannins and phenols (Santos et al 1994). Additional studies on callus and root extracts of these different species have shown the presence of phyllemblin, a tannin which has antimicrobial activity, of possible hydrolyzable tannins 1.…”

Analgesic and antipyretic effects of <i>Sansevieria trifasciata</i> leaves

“…The formalin pain test is useful for evaluating the mechanism of pain and analgesia. Drugs which act centrally, such as narcotic analgesics, inhibits both phases of pain in this model peripherally acting drugs, such as acetylsalicylic acid or indomethacin, only inhibit the late phase (Santos et al, 1994). The water extract of S. trifasciata did not show any significant effect on brewer's yeast-induced fever in rat; however EtOH extract (200 mg/kg) significantly reversed yeast-induced pyrexia.…”