In vitro culture of Phyllanthus stipulatus (Euphorbiaceae)

Catapan, Elizabete; Otuki, Michel Fleith; Viana, Ana Maria

doi:10.1590/s0100-84042001000100003

Cited by 16 publications

(15 citation statements)

References 26 publications

(20 reference statements)

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“…Similar trend of callus formation was also recorded in nodal explants on equimolar concentrations of 2, 4-D, NAA and IAA (Table 3) with significant differences. Concurrent results were reported for establishment of callus in P. urinaria, P. abnormis and P. caroliniensis (Catapan et al 2000(Catapan et al , 2002. Therefore, 2, 4-D seems to be superior over other auxins when used singly for callus formation in Phyllanthus sp.…”

Section: Callus Induction and Growth Of Callussupporting

confidence: 52%

“…BA appears to be superior compared to Kin and TDZ for callus induction in P. amarus. The use of MS medium containing BA was also shown to be effective for callus induction from nodal explant in P. stipulatus (Catapan et al 2001).…”

Section: Callus Induction and Growth Of Callusmentioning

confidence: 97%

“…In vitro propagation techniques have been reported for P. caroliniensis (Catapan et al 2000), P. urinaria (Catapan et al 2002), and P. stipulatus (Catapan et al 2001). Synthetic seeds of alginate encapsulated shoot tips were reported in P. amarus (Singh et al 2006).…”

Section: Introductionmentioning

confidence: 99%

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Thidiazuron-induced shoot organogenesis and production of hepatoprotective lignan phyllanthin and hypophyllanthin in Phyllanthus amarus

Nitnaware

Naik

Nikam

2010

Plant Cell Tiss Organ Cult

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Callus cultures from nodal and leaf explants of Phyllanthus amarus were established on Murashige and Skoog (MS) medium with various combinations of auxins and cytokinins. The leaf-derived callus induced on 2.26 lM 2,4-dichlorophenoxyacetic acid (2, 4-D) ? 2.32 lM Kinetin (Kin) upon transfer to medium containing thidiazuron (TDZ) exhibited higher shoot regeneration (32.4 ± 1.3 shoots per culture). Four-week-old shoots rooted readily on 1.5 lM Indol acetic acid (IAA)-containing medium and were successfully acclimatized with 98% survival. The lignans, Phyllanthin (PH) and Hypohyllanthin (HPH), of leaf extracts from naturally grown plants were identified by using TLC, HPLC and H1-NMR. The PH and HPH production in the regenerated shoots was compared to their production in callus cultures, plants under field conditions and in naturally grown plants. The regenerated shoots on MS ? 2.27 lM TDZ produced about two times higher PH and HPH than the leaves of naturally grown plant. The present study provides a useful system for further studies on in vitro morphogenesis, elicitor-assisted production of PH and HPH and A. rhizogenes-mediated genetic transformation in Phyllanthus amarus.

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Section: Callus Induction and Growth Of Callussupporting

confidence: 52%

Section: Callus Induction and Growth Of Callusmentioning

confidence: 97%

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Thidiazuron-induced shoot organogenesis and production of hepatoprotective lignan phyllanthin and hypophyllanthin in Phyllanthus amarus

Nitnaware

Naik

Nikam

2010

Plant Cell Tiss Organ Cult

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“…BAP played a major distinctive role in the shoot induction in nodal explants. Multiple shoot induction by using BAP at different concentrations has been well reported in several plants; for example, Ocimum spp (Pattnaik and Chand, 1996), Withania somnifera (Manickam et al, 2000), Phyllanthus carolinierisis (Catapan et al, 2000) and Piper spp. (Bhar et al, 1995).…”

Section: Resultsmentioning

confidence: 98%

In vitro micropropagation of Orthosiphon aristatus (Blume) Miq.

Reshi¹,

Sudarshana²

2015

J. Med. Plants Res.

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The present study deals with the mass propagation of Orthosiphon aristatus -a multipurpose highly valuable ethnomedicinal shrub. Stem and inflorescence explants were inoculated into the Murashige and Skoog medium (MS medium) fortified with different concentrations and combinations of auxins and cytokinins. The maximum number of shoots achieved was 25 ± 0.78 and 17 ± 0.67 at 5 mg/l benzylaminopurine (BAP) + 2.5 mg/l kinetin (kn) and 3mg/l BAP + 1.5mg/l Kn from nodal and inflorescence explants, respectively. For in vitro rhizogenesis, the elongated micro shoots were aseptically excised and were transferred to the half strength MS liquid medium supplemented with different concentrations of Indole-3 acetic acid and Indole butyric acid (IAA and IBA). The best rooting was achieved at 2 mg/l IBA giving maximum of 12 ± 0.67 roots per shoot. The well established plantlets were hardened by transferring them to the poly cups containing sterile soil and vermicompost in the ratio of 1:1. After 3 weeks of hardening, established plantlets were transferred to the field with 95% survivability.

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“…In a study to evaluate their ability to support the establishment of callus culture in the medicinal plant Phyllanthus stipulatus, Catapan et al (2001) tested several basal salt formulations including MS, B5, AR (Anderson, 1978), W, SH, KM (Kao and Michayluk, 1975) and WPM media. They observed shoot initiation in the various salt formulations tested; however, rhizogenesis was completely inhibited except on MS and WPM; whereas, callus initiation occurred only on the MS formulation.…”

Section: Discussionmentioning

confidence: 99%

Basal Salt Requirements Differ According to Culture Stage and Cultivar in Date Palm Somatic Embryogenesis

Al-Khayri¹

2011

American Journal of Biochemistry and Biotechnology

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Problem statement: Various media formulations differing in basal salt composition are arbitrary selected to provide essential nutrients for plant in vitro cultures. Evidence suggests that in vitro response to various media formulations varies among genotypes and depends on the culture stage. This study examined the efficacy of five basal salt formulations on callus growth and somatic embryogenesis in date palm Phoenix dactylifera L. using three commercial cultivars, Khusab, Berny and Barhee. Approach: Callus from shoot tip explants maintained on MS medium was introduced to various media formulations including SH, W, MS, WPM and NN media containing 53.7 μM NAA and 7.4 μM 2iP. To assess the effect on callus growth, fresh callus weight was measured 4 and 8 weak later. To evaluate embryogenesis response, callus was transferred to hormone-free media corresponding to those during callus growth stage. Results: The optimum medium formulation varied according to cultivar and culture stage. Extending callus growth to 8 weak allowed for greater discernment of differences as compared to 4 weak because of the inherent slow growth nature of date palm callus. The best callus growth was achieved in cv. Khusab using W and WPM media, cv. Berny using SH and NN medium and cv. Barhee using SH, W and MS media. An optimal medium for callus growth was not necessarily the best for somatic embryogenesis. The highest regeneration percentage in cv. Berny occurred on WPM medium, cv. Khusab on W medium and cv. Barhee using W and WPM media. The highest number of resultant embryos was achieved in cv. Khusab using W and SH media, cv. Berny on WPM and MS media and cv. Barhee using W and SH media. Conclusion: This study provides important information to optimize medium formulation in micropropagation protocols of various date palm cultivars, particularly recalcitrant genotypes. It showed that the best basal salt formulation differed among date palm genotypes and suggested culture stage-specific requirement.

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In vitro culture of Phyllanthus stipulatus (Euphorbiaceae)

Cited by 16 publications

References 26 publications

Thidiazuron-induced shoot organogenesis and production of hepatoprotective lignan phyllanthin and hypophyllanthin in Phyllanthus amarus

Thidiazuron-induced shoot organogenesis and production of hepatoprotective lignan phyllanthin and hypophyllanthin in Phyllanthus amarus

In vitro micropropagation of Orthosiphon aristatus (Blume) Miq.

Basal Salt Requirements Differ According to Culture Stage and Cultivar in Date Palm Somatic Embryogenesis

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