Analgesic and anti-inflammatory activity of root bark of Grewia asiatica Linn. in rodents

Paviaya, Udaybhan Singh; Kumar, Parveen; Wanjari, Manish; Thenmozhi, S.; Balakrishnan, B.

doi:10.4103/0257-7941.122998

Cited by 30 publications

(15 citation statements)

References 17 publications

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“…1). Anti-inflammatory and analgesic activity of G. asiatica root bark extracts were rightly attributed to their phytochemicals including phenolics and flavonoids by [39]. The study further argued methanolic extracts of G. asiatica root bark to induce 59% inhibition in paw edema at equal concentration as has been reported in this study.…”

Section: Anti-oedematous Activity Of G Asiatica Extractssupporting

confidence: 81%

Anticancer and anti-inflammatory perspectives of Pakistan’s indigenous berry Grewia asiatica Linn (Phalsa)

Qamar

Akhtar

Sestili

et al. 2020

JBR

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BACKGROUND: Berries indigenously grown in Asia are known for their diversified nutritional and health promoting properties. Establishing a link between berry consumption and their classical uses in health management however requires detailed research in exploring varied biochemical factors and their therapeutic role in averting risks of chronic disorders. OBJECTIVE: The present study was aimed at evaluating anti-inflammatory and anticancer responses of fruit extracts of Grewia asiatica locally known as Phalsa. METHODS: Dichloromethane, methanol and 50% hydro-methanolic fractions of fruit were evaluated for polyphenols characterization, quantification and antioxidant assays. Anti-inflammatory and anti-nociceptive responses of fruit extracts were evaluated in rats and mice models, respectively, and cytotoxic activities were measured against MCF-7, HeLa, HEp-2, NCI-H522, HEK-293 cancer cell cultures. RESULTS: Phenolics quantification and biological study data suggested 50% hydro-methanolic extracts as maximum carrier of flavonoids (7.92 mgQE/g), anthocyanins (8.1 mg/Kg) and tannins (187.2 mgGAE/g) that significantly (p < 0.05)resulted in higher oxidation inhibition (IC50 41.1 ug/ml), paw edema inhibition (68-74%) and pain mediation in neurogenic phase(31-62%) when administrated at the rate of 400 mg/kg b.w. Maximum cytotoxic activity of G. asiatica (50% hydromethanolic extracts) was observed against MCF-7 (IC50 34.9 ug/mL), HEp-2 (IC50 80.4 ug/mL) and NCI-H522 (IC50 73 ug/mL) cancer cell lines. LC-ESI-MS/MS characterization of hydro-alcoholic fractions bearing potent biological activities revealed Gallic acid, Ellagic acid, Quinic acid, Calycosin, Vidalenolone, Quercetin, Myricetin, Liquitrigenin and 6-aldehydo-isoophiopogonone. Human equivalent doses of the extracts calculated on the basis of total phenolic contents for anti-inflammatory and nociceptive assays were in range between 6.2-15.8 mg/kg b.w., and 3.1-7.9 mg/kg b.w., respectively.

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Section: Anti-oedematous Activity Of G Asiatica Extractssupporting

confidence: 81%

Anticancer and anti-inflammatory perspectives of Pakistan’s indigenous berry Grewia asiatica Linn (Phalsa)

Qamar

Akhtar

Sestili

et al. 2020

JBR

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“…HRBC stabilization and resulted a good membrane stabilization of n-hexane extract of Grewia asiatica (40.89%, 73.57%, 78.23%, 80.07%, 80.91% and 51.95%) at (50 µg/ml, 100µg/ml, 200μg/ml, 400μg/ml, 600μg/ml and 800μg/ml) than methanol extract of both species. The result of the both species possessed similar [10].Ethanolic extract of leaves of Grewia asiatica reduces in blood glucose level in on alloxan induced diabetic wister rats [11].…”

Section: Resultsmentioning

confidence: 98%

A Comparative Study on Phytochemical And Biological Activities of two Grewia Species

Khanal

Raut

Kafle

2016

J. Manmohan Memorial Inst. Health. Sci.

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BackgroundMedicinal plants are currently in considerable significance view due to their special attributes as a large source of therapeutic phytochemicals that may lead to the development of novel drugs. Herbs are staging a comeback and herbal 'renaissance' is happening all over the globe. The herbal products today symbolize safety in contrast to the synthetics that are regarded as unsafe to human and environment. It has been estimated that in developed countries such as United States, plant drugs constitute as much as 25% of the total drugs, while in fast developing countries such as China and India, the contribution is as much as 80%. Thus, the economic importance of medicinal plants is much more to countries such as Nepal than to rest of the world. Inventorisation of herbal drugs used in traditional and modern medicines for a country like Nepal, appears to be a stupendous task, where a number of well established indigenous or traditional systems, including Ayurveda, Unani, Siddha, Homoeopathy, Tibetan, Amchi, Yoga and Naturopathy are practiced along with modern medicine for the management of total health care system. MethodClassic techniques for the solvent extraction of active constituents from medicinal plant matrices are based on the choice of solvent coupled with the use of heat. Soxhlet extraction is a general and wellestablished technique, which surpasses in performance other conventional extraction techniques. : In this method, finely ground sample of Grewia species were placed in a porous bag or "thimble" made from a strong filter paper or cellulose, which is place, is in thimble chamber of the Soxhlet apparatus. Extraction solvents was heated in the bottom flask, vaporizes into the sample thimble, condenses in the condenser and drip back. When the liquid content reaches the siphon arm, the liquid contents emptied into the bottom flask again and the process is continued. The resultant extracts were used for qualitative phytochemical analysis by color reactions, antibacterial effects by disc diffusion method and Human RBC membrane stabilization method was used to test the anti-inflammatory potential of the phytoconstituents. FindingsTwo species Grewia asiatica and Grewia optiva (family: Tiliaceae) from genus Grewia were studied for their phytochemical and biological properties. The result showed that the extractive value of Grewia optiva was found to be greater (2.56 % in hexane and 7.17 % in methanol) than Grewia asiatica (nhexane 2.24% and methanol 5.04%) respectively. The test for the phytoconstituents for both species showed the presence of glycosides, steroids, tannins, flavonoids, terpenoids and saponins. No any extract of both species at dose of (0.25mg, 0.5mg and 1mg per cup) possessed a zone of inhibition against tested bacterial strains. The potency of n-hexane extract and methanol extract of both species for Human RBC membrane stabilization test were compared with standard Diclofenac potassium. HRBC stabilization and resulted a good membrane stabilization of n-hexane extract of Grew...

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“…The pain sensation in acetic acid induced writhing method is elicited by triggering of localized inflammatory response resulting in release of free arachidonic acid from tissue phospholipid[ 22 ] via cyclooxygenase (COX) and prostaglandin biosynthesis. [ 23 ] The acetic acid induced writhing is therefore associated with increased level of PGE-2 and PGF-2 in peritoneal fluids as well as lipoxygenase products. [ 24 ] The analgesic activity of the extract and its fractions might be due the presence of some phyto-constituents which inhibit prostaglandin synthesis or block pain sensation.…”

Section: Discussionmentioning

confidence: 99%

Analgesic and antioxidant activities of stem bark extract and fractions of Petersianthus macrocarpus

2016

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Background:Petersianthus macrocarpus (Lecythidaceae) is widely used in the folk medicine in Nigeria to relieve pain and fever associated with malaria. This study evaluated the analgesic and antioxidant activities of the methanol extract and fractions of the stem bark of the plant.Materials and Methods:The analgesic activity was determined in mice using hotplate and acetic acid-induced writhing models. Morphine sulphate (5 mg/kg, i.p.) and aspirin (100 mg/ml, p.o.) were used as reference analgesic agents. The antioxidant potential was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical; reducing power, iron chelating properties and determination of total phenolic content.Results:The extract at 200 and 500 mg/kg, produced an insignificant (P > 0.05) increase in pain threshold in hotplate but a significant (P < 0.05) increase at 1000 mg/kg. The extract significantly (P < 0.05) reduced the writhing induced by acetic acid in mice in a dose dependent manner. Fractionation increased the analgesic activities significantly (P < 0.05) in ethyl acetate and aqueous fractions (200 mg/kg). The extract demonstrated strong DPPH radical scavenging activity with IC50 0.05 mg/ml, good reducing power and weak iron chelating activities. The total phenol content was 142.32 mg/gin term of gallic acid. The antioxidant effects were more pronounced in ethyl acetate and aqueous fractions.Conclusion:The findings of the study suggested that the extract has strong analgesic and antioxidant activities which reside mainly in the polar fractions thus confirming the traditional use of the plant to alleviate pains.SUMMARY Analgesic and antioxidant activities of extract and solvent fractions of Petersianthus macrocarpus investigated indicated that extract has analgesic and antioxidant properties that reside mainly in the polar fractions. Abbreviations Used: DMSO: Dimethyl sulphoxide, ANOVA: analysis of variance, EDTA: ethylene diamne tetraacetic acid, SDM: standard deviation of mean, PGE: prostaglandins E, PDF: prostaglandins F.

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Analgesic and anti-inflammatory activity of root bark of Grewia asiatica Linn. in rodents

Cited by 30 publications

References 17 publications

Anticancer and anti-inflammatory perspectives of Pakistan’s indigenous berry Grewia asiatica Linn (Phalsa)

Anticancer and anti-inflammatory perspectives of Pakistan’s indigenous berry Grewia asiatica Linn (Phalsa)

A Comparative Study on Phytochemical And Biological Activities of two Grewia Species

Analgesic and antioxidant activities of stem bark extract and fractions of Petersianthus macrocarpus

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