Analgesic and Anti-inflammatory Activities of Triterpenoid Molecules Isolated from the Leaves of Combretum glutinosum Perr. Ex DC (Combretaceae)

Abstract

Combretum glutinosum Perr. Ex DC (COMBRETACEAE) is a traditional medicinal plant, widely distributed in Senegal and Africa. The aim of that study was to investigate the analgesic and anti-inflammatory activities of two triterpenes, betulonic acid (DN7) and cabraleone (DN12), isolated from the leaves of Combretum glutinosum. Experiments were performed in acetic acid-induced contortions in mice and carrageenan rat paw oedema. DN7 (3 mg/kg, per os) and DN12 (3 mg/kg, per os) significantly prevent contortions in mice. The number of contortions is respectively 30±10 and 32±7 versus 72.6±6.64 in control group (p<0.05, n=5). DN7 (3 mg/kg, per os) significantly prevented the increased rat paw oedema (31.84±6.76 vs 92.72±6.05%) (p<0.05, n=5). DN12 (10 mg/kg, per os) induced rat paw oedema prevention similar to that of acetylsalicylic acid (10 mg/kg, per os) administered in the same conditions (29.28±5.88 vs 30.96±7.25%; ns, n=5). The analgesic and anti-inflammatory activities of triterpenes isolated from C. glutinosum leaves are similar to those of non-steroidal anti-inflammatory drugs such acetylsalicylic acid, justifying the use of this plant in traditional medicine to manage pain and inflammation.

Revue des usages traditionnels, composition chimique et propriétés pharmacologiques de <i>Combretum glutinosum</i> (Combretaceae)

“…Au Sénégal, Sène et al ( 2018) ont étudié les activités analgésique et antiinflammatoire de deux triterpènes (acide bétulonique et cabraléone) isolés des feuilles de C. glutinosum sur des contorsions induites par l'acide acétique chez la souris et sur l'oedème de la patte de rat induit par la carragénine. Leurs activités analgésique et anti-inflammatoire étaient similaires à celles des médicaments anti-inflammatoires non stéroïdiens tels que l'acide acétylsalicylique, ce qui justifie son utilisation en médecine traditionnelle pour traiter la douleur et l'inflammation (Sene et al, 2018).…”

In vivo anti-inflammatory and healing activities of the methanolic fraction of Combretum glutinosum Perr. bark

“…Also, many pharmacological studies have reported the therapeutic potential of C. glutinosum extracts. These studies mostly focus on antioxidant [11], antiparasitics [12][13][14], antidiarrheal [15], antihyperglycemic and cytotoxic [16], antibacterial [17] and anti-inflammatory [18] activities. This study participates to the valorization of traditional medicinal plants and aims to highlight the anti-inflammatory and healing activities of the methanolic fraction of the bark of C. glutinosum.…”

Antimicrobial investigation of ethnobotanically selected guinean plant species

“…Four triterpenoids (betulinol, betulonal, betulonic acid and cabraleone) were isolated from the hexane and dichloromethane extracts of the leaves (Diaye et al, 2017). Two of these compounds (betulonic acid and cabraleone) demonstrated significant analgesic and antiinflammatory activity (Sene et al, 2019). Moreover, betulin and its semisynthetic derivatives such as 28-O-(N-acetylanthraniloyl) betulin have been found to possess considerable antibacterial activity against , S. aureus and E. faecalis (MIC90 of 6.25 µM) (Haque et al, 2014).…”