Anaerobiosis in <i>Echinochloa crus-galli</i> (Barnyard Grass) Seedlings

Rumpho, Mary E.; Kennedy, Robert A.

doi:10.1104/pp.72.1.44

Cited by 48 publications

(30 citation statements)

References 15 publications

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“…By internal amino acid sequence analysis, we have preliminarily identified this polypeptide as enolase (EC 4.2.1.11) (unpublished data). The significance of this finding relative to our previous biochemical studies on the anaerobic metabolism of E. phyllopogon (Rumpho and Kennedy, 1981, 1983a, 1983b) is being pursued.…”

Section: Discussionmentioning

confidence: 99%

“…Vol. 101, 19931983b, glycolysis (Rumpho and Kennedy, 1983a;Kennedy et al, 1987b), the oxidative pentose phosphate pathway (Kennedy et al, 1983;Rumpho and Kennedy, 1983a), lipid metabolism (VanderZee and Kennedy, 1982;Knowles and Kennedy, 1984;Everard and Kennedy, 1985), and mitochondrial activity (Kennedy et al, 1987a;Fox and Kennedy, 1991) operate under anaerobic conditions. Recently, we (Fox and Kennedy, 1991) reported that a complete TCA cycle was present in E. phyllopogon under anoxia and that most TCA cycle enzymes in nitrogen-grown seedlings exhibited in vitro activities comparable to those grown in air.…”

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confidence: 99%

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Constitutive and Inducible Aerobic and Anaerobic Stress Proteins in the Echinochloa Complex and Rice

et al. 1993

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Anaerobic stress resulted in a change i n the protein accumulation patterns in shoots of several Echinochloa (barnyard grass) species and Oryza safiva (1.) (rice) as resolved by two-dimensional gel electrophoresis. O f the six Echinochloa species investigated, E. phyllopogon (Stev.) KOSS, E. muricafa (Beauv.) Fern, E. oryzoides (Ard.) Fritsch Clayton, and E. crus-galli (1.) Beauv. are tolerant of anaerobiosis and germinate i n the absence of oxygen, as does rice.In contrast, E. crus-pavonis (H.B.K.) Schult and F. colonum (1.) Link are intolerant and do not germinate without oxygen. Computer analysis of the protein patterns from the four tolerant species and rice indicated that the anaerobic response is of five classes: class 1 proteins, enhanced under anaerobiosis (9 to 13 polypeptides ranging from 16-68 kD); class 2 proteins, unique to anaerobiosis (1 to 5 polypeptides ranging from 17-69 kD); class 3 proteins, remained constant under aerobiosis and anaerobiosis; class 4 proteins, prominent only i n air and repressed under anoxia (3 to 7 polypeptides ranging from 19-45 kD); and class 5 proteins, unique to aerobiosis (1 to 4 polypeptides ranging from 18-63 kD). In the intolerant species, E. colonum and E. crus-pavonis, no polypeptides were enhanced or repressed under anoxia (class 1 and class 4, respectively), whereas in the tolerant Ecbinochloa species and rice, a total of at least 9 to 13 anaerobic stress proteins and 4 to 7 "aerobic" proteins were noted. lmmunoblotting identified two of the major anaerobic stress proteins as fructose-1,6-bisphosphate aldolase and pyruvate decarboxylase. Based on the differential response of the intolerant species to anaerobiosis, we suggest that another set of genes, whose products may not necessarily be among the major anaerobic stress polypeptides, might confer tolerance in Echinochloa under prolonged anaerobic stress.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Constitutive and Inducible Aerobic and Anaerobic Stress Proteins in the Echinochloa Complex and Rice

et al. 1993

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“…var oryzicola (hereafter, crusgalli and oryzicola, respectively), rice (Oryza sativa L., cv S201) pea (Pisum sativum L., cv Alaska), and wheat (Triticum aestivum L., cv Dawes) were surface-sterilized in 2.5% NaOCl for 10 min, washed three times with glass-distilled H20, and germinated with the appropriate N2 (99.995% N2) or air gas phase as before (13). No bacterial or fungal contamination was detected when an aliquot of the germination solution was incubated on agar plates containing (per liter): 15 g agar, 3 g yeast extract, and 10 g glucose (20). Data Figure 2.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, we reported (13) In previous reports, we have presented information on the structural aspects of anaerobic germination in Echinochloa seeds (22), seedling characteristics and cellular ultrastructure (13), chloroplast ultrastructure (23), and metabolism of anaerobic germination (18)(19)(20). This paper presents information on the respiration and response to respiratory inhibitors of anaerobically grown Echinochloa seedlings.…”

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confidence: 99%