An updated model for nitrate uptake modelling in plants. I. Functional component: cross-combination of flow–force interpretation of nitrate uptake isotherms, and environmental and in planta regulation of nitrate influx

Deunff, Erwan Le; Malagoli, Philippe

doi:10.1093/aob/mcu021

Cited by 21 publications

(16 citation statements)

References 99 publications

(195 reference statements)

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“…In addition to contributing to the understanding of the physiological basis of nutrient uptake in grapevine and the scion/rootstock relationships, the data reported here could be important for the development of N‐uptake models (Malagoli et al. , Le Deunff and Malagoli ) as tools for a more rationale approach to grapevine N‐fertilisation. Data also demonstrate that the scion/rootstock combination can greatly affect nitrate uptake rate; because of the impact that nitrogen fertilisation can have on yield, this implies that a generalisation on the effects of nitrogen supply on the performance of a specific cultivar should be taken with caution.…”

Section: Discussionmentioning

confidence: 96%

Induction of nitrate uptake in Sauvignon Blanc and Chardonnay grapevines depends on the scion and is affected by the rootstock

Tomasi

Monte

Varanini

et al. 2015

Australian Journal of Grape and Wine Research

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Background and AimsNitrate is a major form of inorganic nitrogen present in cultivated soils; however, information on the mechanisms responsible for uptake of the anion in grapevines is scarce.\ud \ud Methods and ResultsThe response to external nitrate was studied in two clones of the cultivars Chardonnay and Sauvignon Blanc. Grapevines, own-rooted or grafted on the two rootstocks SO4 and K5BB, were grown in nutrient solution and, after a period of nitrogen (N)-deprivation, exposed to 0.2 or 1mmol/L nitrate. The uptake was an inducible process, operated by high- and low-affinity transport systems. The magnitude and time dependence of the induction varied as a function of the clone. Uptake rate as a function of nitrate external concentration showed a multiphasic pattern. Although nitrate exposure caused a transient up-regulation of nitrate influx transporter genes, a clear correlation with the rise in nitrate uptake could not be found; in contrast, a negative relationship between changes in uptake rates and expression of a nitrate efflux transporter gene was observed.\ud \ud ConclusionsIn the grapevine, the response of low- and high-affinity transport systems to external nitrate was favoured by the presence of the rootstock and also dependent on the characteristics of the scion. Low-affinity uptake and efflux from root cells conceivably play an important role in the overall N-nitrate acquisition.\ud \ud Significance of the StudyThis study shows that activation of mechanisms involved in nitrate uptake in the grapevine is strongly affected by the scion-rootstock combination. Results may be significant for developing agronomic practices and selection programmes related to nitrogen use in the grapevine

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Section: Discussionmentioning

confidence: 96%

Induction of nitrate uptake in Sauvignon Blanc and Chardonnay grapevines depends on the scion and is affected by the rootstock

Tomasi

Monte

Varanini

et al. 2015

Australian Journal of Grape and Wine Research

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“…Compared with the kinetic parameters of NO 3 − uptake (Santos et al , ), rice varieties with higher V max and lower K m under conditions of low NO 3 − supply also showed higher expression levels of the high‐affinity NTRs OsNRT2.1 and OsNRT2.2 (Bucher et al , ). Even under high NO 3 − supply, high‐affinity transporters may also be active for NO 3 − uptake (Le Deunff & Malagoli, ), indicating that this group of transporters significantly contributes to the acquisition of NO 3 − by plants over a wide concentration range. Although the methods by which HAs cause alterations in the expression of NTRs remain unclear, other authors have also reported this effect in different crops (Quaggiotti et al , ; Jannin et al , ).…”

Section: Discussionmentioning

confidence: 99%

Humic acid differentially improves nitrate kinetics under low- and high-affinity systems and alters the expression of plasma membrane H⁺-ATPases and nitrate transporters in rice

et al. 2016

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Humic acids (HAs) have a major effect on nutrient uptake, metabolism, growth and development in plants. Here, we evaluated the effect of HA pretreatment applied with a nutrient solution on the uptake kinetics of nitrate nitrogen (N-NO 3 − ) and the metabolism of nitrogen (N) in rice under conditions of high and low NO 3 − supply. In addition, the kinetic parameters of NO 3 − uptake, N metabolites, and nitrate transporters (NRTs) and the plasma membrane (PM) H + -ATPase gene expression were examined. The plants were grown in a growth chamber with modified Hoagland and Arnon solution until 21 days after germination (DAG), and they were then transferred to a solution without N for 48 h and then to another solution without N and with and without the addition of HAs for another 48 h. After this period of N deprivation, the plants received new nutrient solutions containing 0.2 and 2.0 mM N-NO 3 − . Treatment of rice plants with HA promoted the induction of the genes OsNRT2.1-2.2/OsNAR2.1 and some isoforms PM H + -ATPase in roots. The application of HAs differentially modified the parameters of the uptake kinetics of NO 3 − under both concentrations. When grown with 0.2 mM NO 3 − , the plants pretreated with HA had lower K m and C min values as well as a higher V max /K m ratio. When grown with 2 mM NO 3 − , the plants pretreated with HA had a higher V max value, a greater root and shoot mass, and a lower root/shoot ratio. The N fractions were also altered by pretreatment with HA, and a greater accumulation of NO 3 − and N-amino was observed in the roots and shoots, respectively, of plants pretreated with HA. The results suggest that pretreatment with HA modifies root morphology and gene expression of PM H + -ATPases and NO 3 − transporters, resulting in a greater efficiency of NO 3 − acquisition by high-and low-affinity systems.

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“…In winter oilseed rape, nitrogen uptake efficiency needs to be investigated primarily at the beginning of the crop cycle, since a quarter to a third of the total N uptake occurs before winter (Cramer 1993) and most of the root system is fully developed before flowering (Barraclough 1989;Rahman and McClean 2013;Le Deunff and Malagoli 2014). Generally, the absorption of mineral ions by the plant depends primarily on their availability, which is defined by the quantity of ions present in the soil, the capacity for ion exchange between soil and solute, and the ability of the roots to take up the minerals.…”

Section: Nitrogen Uptake Efficiency Must Be Improved To Maintain a Himentioning

confidence: 99%

“…This phase is characterized by a massive change in source-sink relationships. During flowering, the vegetative plant segments switch from being sink organs to source organs, with associated modifications in the N fluxes from older to younger tissues and to the reproductive organs (Le Deunff and Malagoli 2014). Labeling studies in Arabidopsis thaliana (Taylor et al 2010) and B. napus (Rossato et al 2001;Malagoli et al 2005a) showed that the N accumulated in the seeds at harvest originates mainly from the degradation of proteins in vegetative plant segments.…”

Section: The Post-anthesis Nitrogen Balance Between Source Organs Andmentioning

confidence: 99%

Nitrogen use efficiency in rapeseed. A review

Bouchet

Laperche

Bissuel-Bélaygue

et al. 2016

Agron. Sustain. Dev.

173

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Mineral nitrogen fertilization has improved crop yield over the last century but has also caused air and water pollution. Reduction of nitrogen inputs and maintaining high yields are therefore essential to ensure a more sustainable agriculture. Improving the nitrogen use efficiency (NUE) of crops is therefore needed. Rapeseed, Brassica napus, depends on nitrogen fertilization due to its low NUE, with the ratio of plant nitrogen content to nitrogen supplied often not exceeding 60 %. Here, we review the major phenotypic traits associated with NUE in B. napus, with special emphasis on winter oilseed rape. We discuss the genetic diversity available and potential breeding strategies. The major points are the following: (1) rapeseed seed yield elaboration is complex, with overlapping phases of nitrogen uptake and remobilization during the crop cycle; (2) traits related to nitrogen uptake, such as root length and the amount of nitrogen absorbed after flowering, and traits related to nitrogen remobilization, such as the "staygreen" phenotype, have been identified as possible levers to improve NUE in rapeseed; (3) a substantial body of studies investigating the genetic control of NUE traits have already published and potential candidate genes identified; and (4) rapeseed genetic diversity may be enriched by exploiting interpopulation genetic variation and the closely related gene pools of Brassica rapa and Brassica oleracea.

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An updated model for nitrate uptake modelling in plants. I. Functional component: cross-combination of flow–force interpretation of nitrate uptake isotherms, and environmental and in planta regulation of nitrate influx

Cited by 21 publications

References 99 publications

Induction of nitrate uptake in Sauvignon Blanc and Chardonnay grapevines depends on the scion and is affected by the rootstock

Induction of nitrate uptake in Sauvignon Blanc and Chardonnay grapevines depends on the scion and is affected by the rootstock

Humic acid differentially improves nitrate kinetics under low- and high-affinity systems and alters the expression of plasma membrane H⁺-ATPases and nitrate transporters in rice

Nitrogen use efficiency in rapeseed. A review

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An updated model for nitrate uptake modelling in plants. I. Functional component: cross-combination of flow–force interpretation of nitrate uptake isotherms, and environmental and in planta regulation of nitrate influx

Cited by 21 publications

References 99 publications

Induction of nitrate uptake in Sauvignon Blanc and Chardonnay grapevines depends on the scion and is affected by the rootstock

Induction of nitrate uptake in Sauvignon Blanc and Chardonnay grapevines depends on the scion and is affected by the rootstock

Humic acid differentially improves nitrate kinetics under low- and high-affinity systems and alters the expression of plasma membrane H+-ATPases and nitrate transporters in rice

Nitrogen use efficiency in rapeseed. A review

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Humic acid differentially improves nitrate kinetics under low- and high-affinity systems and alters the expression of plasma membrane H⁺-ATPases and nitrate transporters in rice