An unusual occurrence of plasmid-mediated bla OXA-23 carbapenemase in clinical isolates of Escherichia coli from India

Paul, Deepjyoti; Ingti, Birson; Bhattacharjee, Dibyojyoti; Maurya, Anand Prakash; Dhar, Debadatta; Chakravarty, Atanu; Bhattacharjee, Amitabha

doi:10.1016/j.ijantimicag.2017.01.012

Cited by 19 publications

(15 citation statements)

References 11 publications

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“…We observed an average of 14 (11–18) horizontally transferred genes per genome from K. pneumoniae to A. baumannii . For example, the genome of A. baumannii strain ZW85-1 showed the presence of 18 horizontally transferred genes; in comparison, strain AB0057 contained 11 genes that were transferred (Table S3).…”

Section: Resultsmentioning

confidence: 96%

“…The E. coli strain ( E. coli 521) was recovered from a urine sample of an elderly female and possessed other ß-lactamases (16). Moreover, Paul et al described the presence of fourteen E. coli isolates harboring bla OXA-23 obtained from patients hospitalized in the intensive care unit (18). In P. mirabilis strain CFO239, bla OXA-23 was inserted into the chromosome (43), while in the extended-spectrum β-lactamase, ESBL, P. mirabilis strain 4969, the authors suggested a plasmid location of bla OXA-23 (21).…”

Section: Resultsmentioning

confidence: 99%

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Interspecies DNA acquisition by a naturally competentAcinetobacter baumanniistrain

Traglia

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Dotto

et al. 2018

Preprint

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37Acinetobacter baumannii is a human pathogen that frequently acquires antibiotic 38 resistance genes leading to the emergence of multi-drug-resistant (MDR) strains. 39To investigate the role of transformation in the acquisition of resistance 40 determinants by this species, the susceptible strain A118 was exposed to 41 genomic DNA of carbapenem-resistant Klebsiella pneumoniae (CRKp). Resistant 42 transformants were obtained and an increase in the resistance level to all β-43 lactam antibiotics was observed. Whole genome analysis of transformant clones 44 demonstrated the acquisition of CRKp DNA. The most frequently acquired genes 45 correspond to mobile elements, antibiotic resistance genes, and operons 46 involved in metabolism. Bioinformatic analyses and in silico gene flow prediction 47

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Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

Interspecies DNA acquisition by a naturally competentAcinetobacter baumanniistrain

Traglia

Place

Dotto

et al. 2018

Preprint

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“…Consequently, the molecular tests that are commercially available for the detection of CPE usually do not include bla OXA-23 and bla OXA-58 genes in their targeted genes (19,20). However, OXA-23-and OXA-58-producing P. mirabilis isolates have been recently described not only in Europe (28)(29)(30)(31) but also in other enterobacterial isolates from different countries and continents: Turkey (32), Africa (33), and India (34). It has been suggested (30) that their prevalence might be underestimated due to detection difficulties related to (i) the very weak hydrolysis activity of these carbapenemases and (ii) the intrinsic decreased susceptibility to imipenem (but not ertapenem) of P. mirabilis.…”

Section: Resultsmentioning

confidence: 99%

“…The Amplidiag CarbaRϩVRE assay is the first assay able to detect the five most common carbapenemases and the OXA carbapenemases preferentially found in A. baumannii. The use of this assay would allow identification of these "Acinetobacter-related" enzymes not only in P. mirabilis but also in other enterobacterial isolates (32)(33)(34), allowing evaluation of the current spread of these resistance determinants and assessment of the risk of further dissemination of these enzymes in Enterobacteriaceae.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the Amplidiag CarbaR+VRE Kit for Accurate Detection of Carbapenemase-Producing Bacteria

et al. 2018

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As carbapenemase-producing Gram-negative bacilli (CP-GNB) (, , and spp.) are becoming a major public health issue, there is an urgent need for accurate and fast diagnostic tests. The Amplidiag CarbaR+VRE assay is a multiplex nucleic acid-based diagnostic test intended for the detection of CP-GNB and vancomycin-resistant enterococci (VRE) from cultured colonies. We have evaluated its ability to detect carbapenemase genes in 100 well-characterized GNB and in 200 consecutive enterobacterial isolates with reduced susceptibility to carbapenems that were referred to the French National Reference Center for carbapenem resistance. The assay has been validated on purified DNA but also directly on colonies. The Amplidiag CarbaR+VRE assay could detect all KPC, NDM, VIM, IMP, and OXA-48-like variants tested and all acquired carbapenem-hydrolyzing oxacillinases from (OXA-23, OXA-24/-40, and OXA-58) as well as the overexpressed chromosomally encoded OXA-51-like β-lactamase associated with an upstream inserted IS However, as claimed by the manufacturer, other carbapenemases such as GES-like carbapenemases (GES-2, GES-5, and GES-14), GIM-1, AIM-1, SPM-1, DIM-1, OXA-198 in , or OXA-143-like in were not detected. Amplidiag CarbaR+VRE's performance values were high (100% sensitivity and 99% specificity) as it could detect the five major carbapenemases-NDM, VIM, IMP, KPC, and OXA-48-as well as OXA-type carbapenemases from spp. that are currently emerging also among and other enterobacterial isolates. It can provide a result directly from colonies growing on Mueller-Hinton (MH) agar or on selective screening medium in less than 2 h. Further evaluations are now necessary to determine the performance values directly on rectal swabs.

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“…The spread of an OXA-23-producing clone of P. mirabilis was first revealed during a survey conducted between 1996 and 1999 in France (3). Later, the bla OXA-23 gene was detected sporadically in Escherichia coli in Singapore and India and, more recently, in P. mirabilis from Finland (4)(5)(6). Besides, P. mirabilis strains with a chromosomally integrated or plasmid-borne bla OXA-58 gene were characterized in Belgium and in Germany, respectively (7,8).…”

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confidence: 99%

Carbapenem-Susceptible OXA-23-Producing Proteus mirabilis in the French Community

Potron

Hocquet

Triponney

et al. 2019

Antimicrob Agents Chemother

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Nineteen Proteus mirabilis isolates producing the carbapenemase OXA-23 were recovered over a 2-year period in 19 French hospitalized patients, of whom 12 had community onset infections. The isolates exhibited a slightly reduced susceptibility to carbapenems. Whole-genome analysis revealed that all 19 isolates formed a cluster compared to 149 other P. mirabilis isolates. Because of its susceptibility to carbapenems, this clone may be misidentified as a penicillinase producer while it constitutes a reservoir of the OXA-23-encoding gene in the community.

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An unusual occurrence of plasmid-mediated bla OXA-23 carbapenemase in clinical isolates of Escherichia coli from India

Cited by 19 publications

References 11 publications

Interspecies DNA acquisition by a naturally competentAcinetobacter baumanniistrain

Interspecies DNA acquisition by a naturally competentAcinetobacter baumanniistrain

Evaluation of the Amplidiag CarbaR+VRE Kit for Accurate Detection of Carbapenemase-Producing Bacteria

Carbapenem-Susceptible OXA-23-Producing Proteus mirabilis in the French Community

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