An ultrastructural study of the human micropolycystic ovary

Schuchner, Elsa B.; Ballester, Benoît; Lloret, A. Pérez

doi:10.1530/jrf.0.0530019

Cited by 2 publications

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“…Previous studies have defined ultrastructural changes in follicular cells in PCOS ( 20 , 21 , 22 ); however, prior to the present study, no study identifying which stages of folliculogenesis exhibit these ultrastructural changes in PCOS had existed. In this study, we showed irregularities and thickening and ondulation in the basement membrane of granulosa cells in most of the multilaminar and secondary follicles in the PCOS group.…”

Section: Discussionmentioning

confidence: 77%

GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome

et al. 2018

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Background:Polycystic ovary syndrome is the most frequently seen endocrine disorder in women of reproductive age with a prevalence of about 10%.Aims:To investigate the efficiency of growth differentiation factor 9 and bone morphogenetic protein 15 during folliculogenesis in a dehydroepiandrosterone-induced mouse Polycystic ovary syndrome model.Study Design:Animal experimentation.Methods:Mice were divided into 3 groups: control, vehicle and Polycystic ovary syndrome. Polycystic ovary syndrome model mice were developed by the injection of dehydroepiandrosterone dissolved in 0.1 mL of sesame oil. Ovarian tissues were examined for growth differentiation factor 9 and bone morphogenetic protein 15 using immunofluorescent labelling and electron microscopic examinations.Results:The immunoreactivity of growth differentiation factor 9 and bone morphogenetic protein 15 proteins decreased (p<0.05) in the Polycystic ovary syndrome group (27.73±8.43 and 24.85±7.03, respectively) compared with the control group (33.72±11.22 and 31.12±11.05, respectively) and vehicle group (33.95±10.75 and 29.99±10.72, respectively). Apoptotic changes were observed in granulosa cells, lipid vacuoles increased in Theca cells and thickening and irregularities were noted in the basal lamina of granulosa cells. An increased electron density in the zona pellucida in some of the multilaminar primary and secondary follicles in the Polycystic ovary syndrome model was also observed at the ultrastructural level.Conclusion:These results suggest that the decrease in the growth differentiation factor 9 and bone morphogenetic protein 15 expression initiated at the primary follicle stage effect the follicle development and zona pellucida structure and may cause subfertility or infertility in Polycystic ovary syndrome.

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Section: Discussionmentioning

confidence: 77%

GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome

et al. 2018

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Polycystic Ovarian Disease — Current Concepts

Biggs¹

1981

Aust NZ J Obst Gynaeco

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The Stein Leventhal syndrome, non commonly referred to a4 polycystic ovarian disease (PCOD) has provided a focus for application of n o \ methods of investigation of the ovary. The first description of the condition referred to 7 women; subsequent large-scale studies have confirmed menstrual irregularity, hirsutism and infertility as the principal symptoms.PCOD has been shown to be associated with certain histopathological changci in the ovaries, notably capsular thickening, subcapsular follicular c y j t \ and hyperplasia of the theca interna, with or without follicular atresia.The development of urine hormone assays allowed the endocrine bash of PCOD to be described. Urine determinations have now been largely replaced by assays in biood and profiles of hormone changes in PCOD have been proposed. The significant changes are high, fluctuating levels of luteiniring hormone, r a i d oestrone and androgen concentrations. Oestradiol and follicle stimulating hormone levels are often reduced. Other abnormalities underlying PCOD include defects in

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An ultrastructural study of the human micropolycystic ovary

Abstract: The basement membrane of follicles in the micropolycystic ovaries of infertile women was thickened compared to that in the ovaries of normal women or those with typical polycystic ovaries.

Cited by 2 publications

References 4 publications

GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome

GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome

Polycystic Ovarian Disease — Current Concepts

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