An ultrasensitive sandwich-type electrochemical immunosensor based on signal amplification strategy of gold nanoparticles functionalized magnetic multi-walled carbon nanotubes loaded with lead ions

Li, Faying; Han, Jian; Jiang, Liping; Wang, Yulan; Li, Yueyun; Dong, Yunhui; Wei, Qin

doi:10.1016/j.bios.2015.01.049

Cited by 85 publications

(28 citation statements)

References 52 publications

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“…Multi-walled carbon nanotubes (MWCNTs) are appealing components for various hybrid materials due to their remarkable electrical, thermal, and mechanical properties. These advantages make MWCNTs can be widely used for manufacturing nanodevices and developing multifunctional hybrid materials [23][24][25]. Hybridization of WS 2 with MWCNTs provides fantastic electrical and mechanical properties for electrochemical sensor applications.…”

Section: Introductionmentioning

confidence: 99%

An electrochemical biosensor for DNA detection based on tungsten disulfide/multi-walled carbon nanotube composites and hybridization chain reaction amplification

Shuai

Liu

et al. 2016

Sensors and Actuators B: Chemical

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Section: Introductionmentioning

confidence: 99%

An electrochemical biosensor for DNA detection based on tungsten disulfide/multi-walled carbon nanotube composites and hybridization chain reaction amplification

Shuai

Liu

et al. 2016

Sensors and Actuators B: Chemical

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“…Here, patterns are written with fluorescently labeled AFP (as antigen) on the surfaces modified with anti-AFP (as antibody). In different studies, many selective biosensors have been developed based on antigen-antibody interactions [22,30,31,[33][34][35][36][37]. The technique used in this work provides a new platform for the fabrication of clinical immunosensors, which is applicable for the fast and effective diagnosis of HCC.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of click chemistry microarrays for immunosensing of alpha-fetoprotein (AFP)

Dadfar¹,

Sekula-Neuner²,

Trouillet³

et al. 2019

Beilstein J. Nanotechnol.

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The level of cancer biomarkers in cells, tissues or body fluids can be used for the prediction of the presence of cancer or can even indicate the stage of the disease. Alpha-fetoprotein (AFP) is the most commonly used biomarker for early screening and diagnosis of hepatocellular carcinoma (HCC). Here, a combination of three techniques (click chemistry, the biotin–streptavidin–biotin sandwich strategy and the use of antigen–antibody interactions) were combined to implement a sensitive fluorescent immunosensor for AFP detection. Three types of functionalized glasses (dibenzocyclooctyne- (DBCO-), thiol- and epoxy-terminated surfaces) were biotinylated by employing the respective adequate click chemistry counterparts (biotin–thiol or biotin–azide for the first class, biotin–maleimide or biotin–DBCO for the second class and biotin–amine or biotin–thiol for the third class). The anti-AFP antibody was immobilized on the surfaces via a biotin–streptavidin–biotin sandwich technique. To evaluate the sensing performance of the differently prepared surfaces, fluorescently labeled AFP was spotted onto them via microchannel cantilever spotting (µCS). Based on the fluorescence measurements, the optimal microarray design was found and its sensitivity was determined.

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“…An interesting electrochemical immunosensor for the determination of AFP in serum is the configuration developed by Li et al [36] where secondary antibodies are immobilized onto amplification labels composed of AuNPsfunctionalized magnetic carbon nanotubes (MWCNTs-Fe3O4) with adsorbed lead ions. The presence of AuNPs facilitates the immobilization of the immunoreagent also increasing the electrochemical Nanostructures combining CNTs with electroactive metals are attractive because of the relatively low cost of functionalization and high electrocatalytic effects.…”

Section: Carbon Nanotubes Used As Carriers For Signal Elements In Elementioning

confidence: 99%

Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

Yáñez‐Sedeño

Campuzano

Pingarrón

2017

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Growing demand for developing ultrasensitive electrochemical bioassays has led to the design of numerous signal amplification strategies. In this context, carbon-based nanomaterials have been demonstrated to be excellent tags for greatly amplifying the transduction of recognition events and simplifying the protocols used in electrochemical biosensing. This relevant role is due to the carbon-nanomaterials' large surface area, excellent biological compatibility and ease functionalization and, in some cases, intrinsic electrochemistry. These carbon-based nanomaterials involve well-known carbon nanotubes (CNTs) and graphene as well as the more recent use of other carbon nanoforms. This paper briefly discusses the advantages of using carbon nanostructures and their hybrid nanocomposites for amplification through tagging in electrochemical biosensing platforms and provides an updated overview of some selected examples making use of labels involving carbon nanomaterials, acting both as carriers for signal elements and as electrochemical tracers, applied to the electrochemical biosensing of relevant (bio)markers.

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An ultrasensitive sandwich-type electrochemical immunosensor based on signal amplification strategy of gold nanoparticles functionalized magnetic multi-walled carbon nanotubes loaded with lead ions

Cited by 85 publications

References 52 publications

An electrochemical biosensor for DNA detection based on tungsten disulfide/multi-walled carbon nanotube composites and hybridization chain reaction amplification

An electrochemical biosensor for DNA detection based on tungsten disulfide/multi-walled carbon nanotube composites and hybridization chain reaction amplification

Evaluation of click chemistry microarrays for immunosensing of alpha-fetoprotein (AFP)

Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

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