An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis

Lee, Jaemyeon; Lee, Young Ju; Eun, Young Gyu; Lee, Gi-Ja

doi:10.1016/j.aca.2019.04.030

Cited by 4 publications

(4 citation statements)

References 31 publications

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“…Then, PtNPs were deposited onto the activated SPCE using 1 mM H 2 PtCl 6 •6H 2 O in 0.02 M phosphate buffered saline (PBS, pH 7.4) at a constant potential of −0.2 V for 600 s, after N 2 gas purging for 600 s. The PtNP/SPCE was cleaned with DW and dried. The rGO-CNT nanocomposite was prepared using a previously described method [33]. Briefly, the MWCNT powder (0.15 mg/mL) was added in potassium phosphate buffer (PB, 0.067 M, pH 7.4) and dispersed in the solution for 10 min using an ultrasonic liquid processor (STH-500S; Sonictopia, Cheongju, Korea).…”

Section: Preparation Of Ptnp/rgo-cnt On Ptnp/spcementioning

confidence: 99%

“…The rGO has received significant attention in electrochemical sensors due to the high electrical conductivity, excellent physical and chemical properties, and good stability [30,33]. It can provide a lot of active defect sites with significant turbulence and enlarge layer spacing that can effectively trap active species [43].…”

Section: Preparation and Characterization Of Ptnp/rgo-cnt/ptnp/spcementioning

confidence: 99%

“…Recently, reduced graphene oxide (rGO) and its nanocomposites have been widely used in preparing electrochemical sensors to improve their performance [16,[30][31][32]. In particular, the interconnected networks of rGO have several advantages, including greater surface area, faster charge transfer, and lower mass transport resistance [33]. In addition, metal NP-dispersed graphene networks show enhanced catalytic activity and faster electron transfer rate because they can allow various electroactive ions or target analytes to easily access and diffuse across individual graphene sheets [34,35].…”

Section: Introductionmentioning

confidence: 99%

“…Rasas et al [42] proposed an enzyme-free carbon black-Prussian Blue-based electrochemical sensor for H 2 O 2 sensing in neuroblastoma cells. In our previous study, we prepared an ultrasensitive and stable sensing platform based on AuNP-decorated three-dimensional rGO-carbon nanotube (CNT) nanocomposites by a one-pot electrochemical synthesis for detection of tryptase as a potential biomarker of allergic rhinitis [33].…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical Detection of H2O2 Released from Prostate Cancer Cells Using Pt Nanoparticle-Decorated rGO–CNT Nanocomposite-Modified Screen-Printed Carbon Electrodes

Lee

Kim

et al. 2020

Chemosensors

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In this study, we fabricated platinum nanoparticles (PtNP)-decorated, porous reduced graphene oxide (rGO)–carbon nanotube (CNT) nanocomposites on a PtNP-deposited screen-printed carbon electrode (PtNP/rGO–CNT/PtNP/SPCE) for detection of hydrogen peroxide (H2O2), which is released from prostate cancer cells LNCaP. The PtNP/rGO–CNT/PtNP/SPCE was fabricated by a simple electrochemical deposition and co-reduction method. In addition, the amperometric response of the PtNP/rGO–CNT/PtNP/SPCE electrode was evaluated through consecutive additions of H2O2 at an applied potential of 0.2 V (vs. Ag pseudo-reference electrode). As a result, the prepared PtNP/rGO–CNT/PtNP/SPCE showed good electrocatalytic activity toward H2O2 compared to bare SPCE, rGO–CNT/SPCE, PtNP/SPCE, and rGO–CNT/PtNP/SPCE. In addition, the PtNP/rGO–CNT/PtNP/SPCE electrode exhibited a sensitivity of 206 μA mM−1·cm−2 to H2O2 in a linear range of 25 to 1000 μM (R2 = 0.99). Moreover, the PtNP/rGO–CNT/PtNP/SPCE electrode was less sensitive to common interfering substances, such as ascorbic acid, uric acid, and glucose than H2O2. Finally, real-time monitoring of H2O2 released from LNCaP cells was successfully performed by this electrode. Therefore, we expect that the PtNP/rGO–CNT/PtNP/SPCE can be utilized as a promising electrochemical sensor for practical nonenzymatic detection of H2O2 in live cells or clinical analysis.

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Section: Preparation Of Ptnp/rgo-cnt On Ptnp/spcementioning

confidence: 99%

Section: Preparation and Characterization Of Ptnp/rgo-cnt/ptnp/spcementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Electrochemical Detection of H2O2 Released from Prostate Cancer Cells Using Pt Nanoparticle-Decorated rGO–CNT Nanocomposite-Modified Screen-Printed Carbon Electrodes

Lee

Kim

et al. 2020

Chemosensors

Self Cite

View full text Add to dashboard Cite

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Dual-modal visual/photoelectrochemical all-in-one bioassay for rapid detection of AFP using 3D printed microreactor device

Pan

et al. 2020

Biosensors and Bioelectronics

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Electrochemical Biosensors Based on Carbon Nanomaterials for Diagnosis of Human Respiratory Diseases

Che

Deng

2022

Biosensors

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In recent years, respiratory diseases have increasingly become a global concern, largely due to the outbreak of Coronavirus Disease 2019 (COVID-19). This inevitably causes great attention to be given to the development of highly efficient and minimal or non-invasive methods for the diagnosis of respiratory diseases. And electrochemical biosensors based on carbon nanomaterials show great potential in fulfilling the requirement, not only because of the superior performance of electrochemical analysis, but also given the excellent properties of the carbon nanomaterials. In this paper, we review the most recent advances in research, development and applications of electrochemical biosensors based on the use of carbon nanomaterials for diagnosis of human respiratory diseases in the last 10 years. We first briefly introduce the characteristics of several common human respiratory diseases, including influenza, COVID-19, pulmonary fibrosis, tuberculosis and lung cancer. Then, we describe the working principles and fabrication of various electrochemical biosensors based on carbon nanomaterials used for diagnosis of these respiratory diseases. Finally, we summarize the advantages, challenges, and future perspectives for the currently available electrochemical biosensors based on carbon nanomaterials for detecting human respiratory diseases.

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An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis

Cited by 4 publications

References 31 publications

Electrochemical Detection of H2O2 Released from Prostate Cancer Cells Using Pt Nanoparticle-Decorated rGO–CNT Nanocomposite-Modified Screen-Printed Carbon Electrodes

Electrochemical Detection of H2O2 Released from Prostate Cancer Cells Using Pt Nanoparticle-Decorated rGO–CNT Nanocomposite-Modified Screen-Printed Carbon Electrodes

Dual-modal visual/photoelectrochemical all-in-one bioassay for rapid detection of AFP using 3D printed microreactor device

Electrochemical Biosensors Based on Carbon Nanomaterials for Diagnosis of Human Respiratory Diseases

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