An Ultra-sensitive LC Method for the Simultaneous Determination of Paracetamol, Carbamazepine, Losartan and Ciprofloxacin in Bulk Drug, Pharmaceutical Formulation and Human Serum by Programming the Detector

Abstract

An ultra-sensitive LC method for the simultaneous quantitation of paracetamol, carbamazepine, losartan and ciprofloxacin have been developed and validated following the ICH guidelines at isobestic point and by programming the detector at individual wavelength of each component. −1 respectively. Percent recoveries > 98.37% from pharmaceutical formulation and human serum samples and RSD < 2% for inter-day and intra-day assay were obtained. The method was found to be robust and can be successfully applied for the determination of studied drugs in, pharmaceutical formulations and human serum without interference of excipients or endogenous components of serum.

Validated spectrofluorimetric method for the determination of carbamazepine in pharmaceutical dosage forms after reaction with 4‐chloro‐7‐‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD‐Cl)

“…A literature review revealed that different methods have been reported for the determination of CBZ. These include spectrophotometry , photoinduced fluorimetry , voltammetry , high‐performance liquid chromatography , spectrofluorimetry , micellar electrokinetic capillary chromatography , solid‐phase extraction , chemiluminescence , liquid chromatography , ion chromatography and gas chromatographic methods .…”

Facile and Manifest Liquid Chromatographic Method for the Simultaneous Determination of Enalpril Maleate and NSAIDs in API and Pharmaceutical Formulations

“…Lisinopril, captopril [13,14] and metformin [15] have also been simultaneously determined with NSAIDs. Sultana and Arayne et al, have also developed methods for the simultaneous determination of various co-administered drugs as calcium channel blockers with NSAIDS [16,17] and many other drugs [18][19][20][21][22][23] in raw materials, pharmaceutical formulations…”

HPLC Method for Determination of Paracetamol in Pharmaceutical Formulations and Environmental Water Samples

“…Paracetamol (acetaminophen or N-acetyl-4-aminophenol), is a popular analgesic and antipyretic agent, with the following structural formula 1,2 (Figure 1). 3 , HPLC methods [4][5][6][7][8] , HPLC and UV methods [9][10] , HPTLC methods [11][12][13][14] , LC methods [15][16] , UV-spectrophotometric methods [17][18][19][20][21][22][23][24][25] , amperometric method 26 , fluorimetric method 27 , chemiluminescence method 28 and voltametric methods 29 . These methods are required expensive or sophisticated instruments and not simple for routine analysis.…”