An overview of a diagnostic and epidemiologic reappraisal of cutaneous leishmaniasis in Iran

Abstract

Cutaneous leishmaniasis (CL) is a widespread tropical infection which has a high incidence rate in Iran. Leishmania tropica, the causative agent of anthroponotic cutaneous leishmaniasis (ACL), and Leishmania major, which causes zoonotic cutaneous leishmaniasis (ZCL), are endemic in various parts of Iran with a high incidence rate. The aim of this study was to evaluate the reappraisal of the diagnosis and epidemiology of CL in Iran, by different clinical, parasitological and molecular assays among patients suspected of CL referred to the Department of Parasitology, at the Pasteur Institute of Iran during 2006-2009. Two hundred samples from patients with ulcerative skin lesions were collected, clinical analyses were applied, data questionnaire was completed and samples were examined for CL by using both direct microscopic and culture methods. Moreover, PCR assay was applied for detection of Leishmania species in CL isolates resulting from parasitological assay. Clinical observation revealed that the majority (58%) of lesions was single; double lesions were observed in 22% of patients, and only 20% of CL had multiple lesions. Out of 200 patients, Leishman body was observed in 77 samples (38.5%) by direct smear and 40% by cultivation assay. Most patients (21.3%) had a travel history to the Isfahan province, one of the most important endemic areas of CL located in center of Iran. PCR assay by kDNA indicated 32 and 18 out of 50 isolates respectively had similar patterns with standard L. major and L. tropica. In conclusion, clinical manifestations and an appropriate diagnostic assay with a parallel molecular characterization of CL may lead to a screening evaluation of disease, prognosis, treatment and control strategies.

Epidemiological studies on cutaneous leishmaniasis in Ad- Dawadimi District, Saudi Arabia

“…These finding are similar to those of Al-Tawfiq and Abu Khamsin [7]. Most recent epidemiological studies in Yemen, Saudi Arabia and Israel revealed a similar trend, with single lesions on the face or limbs being the most common form of presentation [7,[9][10][11][12].…”

A Case- Study of Mucosal Leishmaniasis in the Lip

“…Leishmaniasis of the skin may occur anywhere on the body but the usual sites are the exposed parts. Most patients have one or two ulcers, but the lesions may be multiple with various presentations (8). The cases of isolated lip leishmaniasis are seldom reported from Iran (9).…”

Biosynthesis of silver nanoparticles by Leishmania tropica

“…The culture was incubated at 25°C and checked for growth of Leishmania promastigotes for 28 days. Penicillin-G and streptomycin were added to the phosphate buffered saline (PBS) solution utilized in the NNN media culture (Eisenberger and Jaffe, 1999;Farahmand et al, 2008).…”