An Outbreak of a Respiratory Disorder at a Russian Swine Farm Associated with the Co-Circulation of PRRSV1 and PRRSV2

Abstract

We conducted a cross-sectional study to identify the major respiratory pathogen responsible for an outbreak of respiratory disease at a swine farm in West Siberia in 2019. We discovered that the peak of morbidity and mortality coincided with a high level of porcine reproductive and respiratory syndrome virus (PRRSV) 1 and 2-related viremia. Based on longer PRRSV2 viremia, the dominant role of PRRSV2 over PRRSV1 in the outbreak was assumed. Phylogenetic analysis revealed that the PRRSV1 strain belonged to sub-genotype 2—one of the predominant groups of genotype 1 PRRSVs in Russia. A partial open reading frame 7 sequence of the PRRSV2 isolate demonstrated a high identity with modified live vaccine-related strains from Denmark (93%) and wild-type VR2332 (92%). We identified the first instance of PRRSV1/PRRSV2 mixed infection in Russia. This finding indicates that further field investigations are needed to access PRRSV2 epidemiology in eastern Europe.

Prevalence and genetic evolution of porcine reproductive and respiratory syndrome virus in commercial fattening pig farms in China

“…Long-term monitoring of PRRSV will be helpful for more comprehensively elucidating the infection situation at commercial fattening pig farms, providing insights of great significance for the prevention and control of PRRSV. The coexistence of multiple PRRSV subtypes has always been a problem faced by the global pig industry [ 29 – 31 ]. Previous studies have demonstrated that PRRSV-2 has long been considered the predominant species in China, cocirculating with PRRSV-1 [ 11 ].…”

Metagenomic Approach Reveals the Second Subtype of PRRSV-1 in a Pathogen Spectrum during a Clinical Outbreak with High Mortality in Western Siberia, Russia

“…Both ORF5 and ORF7 sequences were chosen for genotyping the detected PRRSV-1. Sanger sequencing and prior sample preparation were conducted as described previously [ 11 ], with several changes. For DNA extraction from the PCR mixture, the Cleanup Mini Kit was used in accordance with the manufacturer’s instructions.…”

Análise de doenças de notificação obrigatória em animais de produção no período de 2018 a 2022: uma revisão sistemática

“…Foram realizados testes diferenciais para outras doenças, entre elas, a PSA, a PSC e para o vírus da Pseudo-raiva, porém os dados não estavam disponíveis no artigo, uma vez que nenhum local testou todos os diagnósticos diferenciais e alguns locais não testaram nenhum. Raev, et al (2020) identificaram os patógenos respiratórios responsáveis por um surto de doença respiratória em uma fazenda de suínos na Sibéria Ocidental em 2019. O RNA viral PRRSV1 (5/9; 50%), PRRSV2 (4/9; 40%) e misto PRRSV1&2 (1/9; 10%) foram detectados em leitões de sete semanas e o PRRSV2 (6/10; 60%) isoladamente, em leitões de 12 semanas.…”